2020
DOI: 10.1371/journal.pone.0238812
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One-step automated bioprinting-based method for cumulus-oocyte complex microencapsulation for 3D in vitro maturation

Abstract: Three-dimensional in vitro maturation (3D IVM) is a promising approach to improve IVM efficiency as it could prevent cumulus-oocyte complex (COC) flattening and preserve its structural and functional integrity. Methods reported to date have low reproducibility and validation studies are limited. In this study, a bioprinting based production process for generating microbeads containing a COC (COC-microbeads) was optimized and its validity tested in a large animal model (sheep). Alginate microbeads were produced… Show more

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Cited by 24 publications
(22 citation statements)
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“…Although bioengineering strategies are becoming widespread in the area of cell culture, including ARTs, to the best of our knowledge such approaches have never been applied in prepubertal oocytes in studies conducted to date, but only in adult oocytes [36,51,[58][59][60][61][62].…”
Section: Discussionmentioning
confidence: 99%
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“…Although bioengineering strategies are becoming widespread in the area of cell culture, including ARTs, to the best of our knowledge such approaches have never been applied in prepubertal oocytes in studies conducted to date, but only in adult oocytes [36,51,[58][59][60][61][62].…”
Section: Discussionmentioning
confidence: 99%
“…The aim of the present study was to mimic as much as possible the physiological follicular conditions, such as COC microencapsulation, for 3D-IVM. The first step was to provide prepubertal COCs with a 3D environment using a one-step automated bioprinting method [36]. As the results show, the applied technique was able to improve prepubertal oocyte nuclear maturation rates and to reduce the incidence of chromosomal abnormalities.…”
Section: Discussionmentioning
confidence: 99%
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“…Recently, second meiotic metaphase oocytes were obtained through multistep in human [ 12 ]. Meanwhile, 3-dimensional (3D) culture techniques have utilized a wide range of approaches such as embedding cells in extracellular matrixes [ 13 , 14 , 15 ], printing 3D scaffolds [ 16 ], 3D bioprinting of biocompatible materials and cells [ 17 ], decellularization/ recellularization of tissues [ 18 ] and also the use of more sophisticated microfluidics technology [ 19 , 20 ].…”
Section: Introductionmentioning
confidence: 99%