One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum

Abstract

a b s t r a c t B-phycoerythrin (B-PE) was separated and purified from microalga Porphyridium cruentum using onestep chromatographic method. Phycobiliproteins in P. cruentum was extracted by osmotic shock and initially purified by ultrafiltration. Further purification was carried out with a SOURCE 15Q exchange column and analytical grade B-PE was obtained with a purity ratio (A 545 /A 280 ) of 5.1 and a yield of 68.5%. It showed a double absorption peaks at 545 nm and 565 nm and a shoulder peak at 498 nm, and displayed a fluorescence emission maximum at 580 nm. The analysis by sodium dodecyl sulfate epolyacrylamide gel electrophoresis (SDSePAGE) showed a bulky band between 18 and 20 kDa which could be assigned to subunits a and b and a low intensity band of 27 kDa assigned to g subunit. Our protocol provides attractive alternative to consider for the purification procedure to obtain analytical grade B-PE at commercial level.

Energy transfer dynamics in B-phycoerythrin from the red alga Porphyridium purpureum

“…4. In the figure, the emission peak of B-PE was located at 575 nm, which was consistent with the reported of Tang et al [39]. However, there was a very weak fluorescence emission peak at 620 nm.…”

Dye-sensitized solar cells based on natural and artificial phycobiliproteins to capture low light underwater

“…C-PC from Spirulina platensis was provided by Xindaze Spirulina Co. Ltd, China. B-PE from Porphyridium was isolated and purified by our laboratory [35].…”

Phycobiliproteins: Molecular structure, production, applications, and prospects

“…Two bilins are linked to conserved cysteine residues at position 84 to the α and β subunits, while other pigments (if present) are bound at additional cysteine sites (α75, α140, β50/61, β155, etc) (Glazer, 1994;Apt et al, 1995;MacColl, 1998;MacColl et al, 1999;Sonani et al, 2015). (Fisher et al, 1980;Ficner et al, 1992;Tang et al, 2016) (Nies and Wehrmeyer, 1980;Rumbeli et al, 1985;Duerring et al, 1990)…”