One step closer to eliminating the nomenclatural problems of minute coccoid green algae: <i>Pseudochloris wilhelmii</i>, <i>gen. et sp. nov</i>. (Trebouxiophyceae, Chlorophyta)

Somogyi, Boglárka; Felföldi, Tamás; Solymosi, Katalin; Flieger, Kerstin; Màrialigeti, Károly; Böddi, Béla; Vörös, Lajos

doi:10.1080/09670262.2013.854411

Cited by 35 publications

(17 citation statements)

References 35 publications

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“…As observed by Somogyi et al [ 74 ] in 18S rDNA trees (albeit with no support), we found that the Parachlorella clade is sister to the other two lineages in most amino acid-based trees; however, this position is occupied by the Marvania clade in the phylogenies inferred from nucleotide data. A recent 18S rDNA study [ 75 ] recovered Pseudochloris wilhelmii and the Parachlorella and Chlorella clades as part of a large assemblage that is sister to Marvania , a topology that contrasts with the finding that Marvania and Pseudochloris are sister taxa in all our analyses.…”

Section: Discussioncontrasting

confidence: 84%

Chloroplast phylogenomic analysis resolves deep-level relationships within the green algal class Trebouxiophyceae

2014

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BackgroundThe green algae represent one of the most successful groups of photosynthetic eukaryotes, but compared to their land plant relatives, surprisingly little is known about their evolutionary history. This is in great part due to the difficulty of recognizing species diversity behind morphologically similar organisms. The Trebouxiophyceae is a species-rich class of the Chlorophyta that includes symbionts (e.g. lichenized algae) as well as free-living green algae. Members of this group display remarkable ecological variation, occurring in aquatic, terrestrial and aeroterrestrial environments. Because a reliable backbone phylogeny is essential to understand the evolutionary history of the Trebouxiophyceae, we sought to identify the relationships among the major trebouxiophycean lineages that have been previously recognized in nuclear-encoded 18S rRNA phylogenies. To this end, we used a chloroplast phylogenomic approach.ResultsWe determined the sequences of 29 chlorophyte chloroplast genomes and assembled amino acid and nucleotide data sets derived from 79 chloroplast genes of 61 chlorophytes, including 35 trebouxiophyceans. The amino acid- and nucleotide-based phylogenies inferred using maximum likelihood and Bayesian methods and various models of sequence evolution revealed essentially the same relationships for the trebouxiophyceans. Two major groups were identified: a strongly supported clade of 29 taxa (core trebouxiophyceans) that is sister to the Chlorophyceae + Ulvophyceae and a clade comprising the Chlorellales and Pedinophyceae that represents a basal divergence relative to the former group. The core trebouxiophyceans form a grade of strongly supported clades that include a novel lineage represented by the desert crust alga Pleurastrosarcina brevispinosa. The assemblage composed of the Oocystis and Geminella clades is the deepest divergence of the core trebouxiophyceans. Like most of the chlorellaleans, early-diverging core trebouxiophyceans are predominantly planktonic species, whereas core trebouxiophyceans occupying more derived lineages are mostly terrestrial or aeroterrestrial algae.ConclusionsOur phylogenomic study provides a solid foundation for addressing fundamental questions related to the biology and ecology of the Trebouxiophyceae. The inferred trees reveal that this class is not monophyletic; they offer new insights not only into the internal structure of the class but also into the lifestyle of its founding members and subsequent adaptations to changing environments.

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Section: Discussioncontrasting

confidence: 84%

Chloroplast phylogenomic analysis resolves deep-level relationships within the green algal class Trebouxiophyceae

2014

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“…The third group of Zab-szék clones, was affiliated with the recently described Chloroparva and the revised Pseudochloris genera (Somogyi et al 2011(Somogyi et al , 2013 with relatively low pairwise similarity values, 97.3-97.9%, and therefore represented additional potential new species or genera. Type strain of Chloroparva pannonica was isolated from a soda pan in the same region close to Zab-szék (Somogyi et al 2011), an additional isolate from this genus was retrieved from Zab-szék itself (ACT 0602) and Pseudochloris isolates also originated from saline (marine) habitats (Henley et al 2004;Somogyi et al 2013). …”

Section: Community Compositionmentioning

confidence: 99%

Unique picoeukaryotic algal community under multiple environmental stress conditions in a shallow, alkaline pan

et al. 2013

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Winter phytoplankton communities in the shallow alkaline pans of Hungary are frequently dominated by picoeukaryotes, sometimes in particularly high abundance. In winter 2012 the ice-covered alkaline Zab-szék pan was found to be extraordinarily rich in picoeukaryotic green algae (42-82 × 10 6 cells ml -1 ) despite the simultaneous presence of multiple stressors (low temperature and light intensity with high pH and salinity). The maximum photosynthetic rate of the picoeukaryote community was 1.4 µg C µg chlorophyll a -1 h -1 at 125 µmol m -2 s -1 .The assimilation rates compared with the available light intensity measured on the field show that the community was considerably light-limited. Estimated areal primary production was 2 180 mg C m -2 d -1 . On the basis of the 18S rRNA gene analysis (cloning and DGGE), the community was phylogenetically heterogeneous with several previously undescribed chlorophyte lineages, which indicates the ability of picoeukaryotic communities to maintain high genetic diversity under extreme conditions.

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“…Genomic DNA was extracted from the isolates with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). PCR was performed with primer pairs Euk328f-Chlo02R [55,56] as described by Somogyi et al [57]. Reactions were carried out in a final volume of 50 μL using 2 μL genomic DNA, 0.2 mM of each deoxynucleotide, 1 U LC Taq (Thermus aquaticus) DNA polymerase (Fermentas, Vilnius, Lithuania), 1 × PCR buffer (Fermentas), 2 mM MgCl2, 0.325 mM of each primer and 20 μg of BSA (Bovine Serum Albumin) (Fermentas).…”

Section: Molecular Characterizationmentioning

confidence: 99%

Screening and Evaluation of Some Green Algal Strains (Chlorophyceae) Isolated from Freshwater and Soda Lakes for Biofuel Production

et al. 2015

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Microalgae are photosynthetic microorganisms that can produce lipids, proteins and carbohydrates in large amounts and within short periods of time and these can be processed into both biofuels and other useful commercial products. Due to this reason microalgae are considered as a potential source of renewable energy; and one of the most important decisions in obtaining oil from microalgae is the choice of species. In this study, the potential of Chlorophyceae species isolated from freshwater and soda lakes in Hungary and Romania (Central Europe) were characterized and evaluated by determining their biomass accumulation, lipid productivity, fatty acid profiles, and biodiesel properties besides protein and carbohydrate productivity. Out of nine strains tested, three accumulated more than 40% dry weight of protein, four accumulated more than 30% dry weight of carbohydrate and the strain Chlorella vulgaris LC8 accumulated high lipid content (42.1% ± 2.6%) with a favorable C16-C18 fatty acid profile (77.4%) as well as suitable biodiesel properties of high cetane number (57.3), low viscosity (4.7 mm 2 /s), lower iodine number (75.18 g I2/100 g), relative cloud point (8.8 °C) and negative cold filter plugging point (−6.5 °C). Hence the OPEN ACCESSEnergies 2015, 8 7503 new strain, Chlorella vulgaris LC8 has potential as a feedstock for the production of excellent quality biodiesel.

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One step closer to eliminating the nomenclatural problems of minute coccoid green algae: Pseudochloris wilhelmii, gen. et sp. nov. (Trebouxiophyceae, Chlorophyta)

Cited by 35 publications

References 35 publications

Chloroplast phylogenomic analysis resolves deep-level relationships within the green algal class Trebouxiophyceae

Chloroplast phylogenomic analysis resolves deep-level relationships within the green algal class Trebouxiophyceae

Unique picoeukaryotic algal community under multiple environmental stress conditions in a shallow, alkaline pan

Screening and Evaluation of Some Green Algal Strains (Chlorophyceae) Isolated from Freshwater and Soda Lakes for Biofuel Production

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