One-step nucleic acid amplification for detecting lymph node metastasis of head and neck squamous cell carcinoma

Peigné, Lucie; Godey, Florence; Gallo, Mathieu; Gall, François Le; Fautrel, Alain; Morçet, Jeff; Jégoux, Franck

doi:10.1016/j.oraloncology.2019.104553

Cited by 9 publications

(8 citation statements)

References 33 publications

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“…Moreover, tumor cells damage the surrounding organs and tissues, thereby causing some facial dysfunctions (such as loose teeth, facial paralysis, restricted mouth opening, pain, and infection), which significantly reduce the patient's quality of life [ 10 ]. The maxillofacial tissue contains a large number of blood vessels and nerves, and the incidence of neck lymph node metastasis and invasion is high [ 11 ]. Its degree of malignancy is mainly related to tumor invasion and metastasis.…”

Section: Introductionmentioning

confidence: 99%

MCU That Is Transcriptionally Regulated by Nrf2 Augments Malignant Biological Behaviors in Oral Squamous Cell Carcinoma Cells

Zuo

et al. 2021

BioMed Research International

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Objective. To clarify the role and molecular mechanism of mitochondrial calcium uniporter (MCU) in the malignant biological behaviors of oral squamous cell carcinoma (OSCC) cells through clinical and cellular experiments. Methods. Immunohistochemistry and qRT-PCR techniques were used to observe the expression of MCU, nuclear factor erythroid 2-related factor 2 (Nrf2), mitochondrial calcium uptake 1 (MICU1), and MICU2 in OSCC and normal tissues. After treatment with si-MCU, spermine, and/or sh-Nrf2, malignant biological behaviors of OSCC cells including proliferation, migration, and apoptosis were detected by clone formation, migration, and mitochondrial membrane potential (MMP) assays. Furthermore, MCU, MICU1, MICU2, Nrf2, and other proteins related to malignant biological behaviors were examined using western blot, immunohistochemistry, and immunofluorescence assays. Results. MCU, Nrf2, and MICU1 were strongly expressed in OSCC as compared to normal tissues, while MICU2 was relatively weakly expressed in OSCC tissues. Knockdown of MCU distinctly weakened proliferation and migration and lowered MMP level in CAL 27 cells. Conversely, its activation reinforced migrated capacity and increased MMP level in CAL 27 cells, which was reversed after cotransfection with sh-Nrf2. After treatment with si-MCU or spermine, Nrf2 expression was not affected in CAL 27 cells. However, MCU expression was distinctly suppressed in CAL 27 cells transfected with sh-Nrf2. Furthermore, knockdown of Nrf2 significantly reversed the increase in expression of MICU1 and MICU2 induced by MCU activation in CAL 27 cells. Conclusion. MCU, as a novel oncogene of OSCC, augments malignant biological behaviors of OSCC cells, which could be transcriptionally regulated by Nrf2.

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Section: Introductionmentioning

confidence: 99%

MCU That Is Transcriptionally Regulated by Nrf2 Augments Malignant Biological Behaviors in Oral Squamous Cell Carcinoma Cells

Zuo

et al. 2021

BioMed Research International

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“…Moreover, molecular methods, which are more time and tissue consuming but also more sensitive, should be tested more widely in this setting. [19][20][21] The postsurgery functional results in the subgroup of patients who underwent a second surgery were not different from those of patients who had node dissection during the same intervention. This can be explained by the low number of patients who did not allow a robust analysis, or by the real absence of differences between these conditions.…”

Section: Discussionmentioning

confidence: 85%

Equivalence Randomized Trial to Compare Treatment on the Basis of Sentinel Node Biopsy Versus Neck Node Dissection in Operable T1-T2N0 Oral and Oropharyngeal Cancer

Garrel

Poissonnet

Plana

et al. 2020

JCO

131

109

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PURPOSE Sentinel node (SN) biopsy is accurate in operable oral and oropharyngeal cT1-T2N0 cancer (OC), but, to our knowledge, the oncologic equivalence of SN biopsy and neck lymph node dissection (ND; standard treatment) has never been evaluated. METHODS In this phase III multicenter trial, 307 patients with OC were randomly assigned to (1) the ND arm or (2) the SN arm (experimental arm: biopsy alone if negative, or followed by ND if positive, during primary tumor surgery). The primary outcome was neck node recurrence-free survival (RFS) at 2 years. Secondary outcomes were 5-year neck node RFS, 2- and 5-year disease-specific survival (DSS), and overall survival (OS). Other outcomes were hospital stay length, neck and shoulder morbidity, and number of physiotherapy prescriptions during the 2 years after surgery. RESULTS Data on 279 patients (139 ND and 140 SN) could be analyzed. Neck node RFS was 89.6% (95% CI, 0.83% to 0.94%) at 2 years in the ND arm and 90.7% (95% CI, 0.84% to 0.95%) in the SN arm, confirming the equivalence with P < .01. The 5-year RFS and the 2- and 5-year DSS and OS were not significantly different between arms. The median hospital stay length was 8 days in the ND arm and 7 days in the SN arm ( P < .01). The functional outcomes were significantly worse in the ND arm until 6 months after surgery. CONCLUSION This study demonstrated the oncologic equivalence of the SN and ND approaches, with lower morbidity in the SN arm during the first 6 months after surgery, thus establishing SN as the standard of care in OC.

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“…The OSNA rapid turnover time may potentially be useful in circumventing the major issues associated with SLN biopsy. Moreover, OSNA is automated and the results are quantifiable; hence, easily reproducible, less operator-dependent with short learning curve[ 201 - 203 ]. Therefore, implementation of OSNA in routine clinical practice may ease the burden on pathologists.…”

Section: Advantages and Disadvantagesmentioning

confidence: 99%

“…An additional challenge is the fact that 36% and 49% of CK19-negative primary tumors have CK19-positive LNs[ 183 ]. Peigné et al [ 203 ] also reported that CK19 mRNA can also be detected by OSNA even in cases with CK19-negative primary lesion.…”

Section: Advantages and Disadvantagesmentioning

confidence: 99%

Role of one-step nucleic acid amplification in colorectal cancer lymph node metastases detection

Crafa¹,

Vanella²,

Catalano³

et al. 2022

WJG

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Current histopathological staging procedures in colorectal cancer (CRC) depend on midline division of the lymph nodes (LNs) with one section of hematoxylin and eosin staining. Cancer cells outside this transection line may be missed, which could lead to understaging of Union for International Cancer Control Stage II high-risk patients. The one-step nucleic acid amplification (OSNA) assay has emerged as a rapid molecular diagnostic tool for LN metastases detection. It is a molecular technique that can analyze the entire LN tissue using a reverse-transcriptase loop-mediated isothermal amplification reaction to detect tumor-specific cytokeratin 19 mRNA. Our findings suggest that the OSNA assay has a high diagnostic accuracy in detecting metastatic LNs in CRC and a high negative predictive value. OSNA is a standardized, observer-independent technique, which may lead to more accurate staging. It has been suggested that in stage II CRC, the upstaging can reach 25% and these patients can access postoperative adjuvant chemotherapy. Moreover, intraoperative OSNA sentinel node evaluation may allow early CRC to be treated with organ-preserving surgery, while in more advanced-stage disease, a tailored lymphadenectomy can be performed considering the presence of aberrant lymphatic drainage and skip metastases.

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One-step nucleic acid amplification for detecting lymph node metastasis of head and neck squamous cell carcinoma

Cited by 9 publications

References 33 publications

MCU That Is Transcriptionally Regulated by Nrf2 Augments Malignant Biological Behaviors in Oral Squamous Cell Carcinoma Cells

MCU That Is Transcriptionally Regulated by Nrf2 Augments Malignant Biological Behaviors in Oral Squamous Cell Carcinoma Cells

Equivalence Randomized Trial to Compare Treatment on the Basis of Sentinel Node Biopsy Versus Neck Node Dissection in Operable T1-T2N0 Oral and Oropharyngeal Cancer

Role of one-step nucleic acid amplification in colorectal cancer lymph node metastases detection

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