One‐Step Nucleic Acid Purification and Noise‐Resistant Polymerase Chain Reaction by Electrokinetic Concentration for Ultralow‐Abundance Nucleic Acid Detection

Ouyang, Wei; Han, Jongyoon

doi:10.1002/ange.201915788

Cited by 5 publications

(6 citation statements)

References 61 publications

(63 reference statements)

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“…7 B) ( Ouyang et al, 2018 ). The coupling of hierarchically selective EK concentrations with microfluidic PCRs allow detection of the lowest number of detectable DNA copy numbers, five copies, which was two orders of magnitude better than the standard method ( Ouyang and Han, 2019 , 2020 ). In another study, Hügle et al (2020) utilised gel electrophoresis to extract nucleic acids, i.e.…”

Section: Potential Biosensors For Point-of-care Sars-cov-2 Detectionmentioning

confidence: 99%

“…There is therefore an urgent need for the development of a rapid and simple method for the highly effective release and enrichment of SARS-CoV-2 RNA, which can be directly used for detection ( He et al, 2017 ; Nyan et al, 2014 ). The ideal strategy could be the integration of RNA release through pre-concentration as well as amplification and detection in portable microfluidic-based cartridges or chips, which could enable point-of-care SARS-CoV-2 testing outside of the clinical diagnostic laboratory; in places such as airports, local emergency departments, and clinics ( Dincer et al, 2019 ; Jin et al, 2018 ; Ouyang and Han, 2020 ).…”

Section: The Unmet Problem For Sars-cov-2 Detectionmentioning

confidence: 99%

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Detection of COVID-19: A review of the current literature and future perspectives

Liu

Wang

et al. 2020

Biosensors and Bioelectronics

353

309

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The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to the coronavirus disease 2019 (COVID-19) worldwide pandemic. This unprecedented situation has garnered worldwide attention. An effective strategy for controlling the COVID-19 pandemic is to develop highly accurate methods for the rapid identification and isolation of SARS-CoV-2 infected patients. Many companies and institutes are therefore striving to develop effective methods for the rapid detection of SARS-CoV-2 ribonucleic acid (RNA), antibodies, antigens, and the virus. In this review, we summarize the structure of the SARS-CoV-2 virus, its genome and gene expression characteristics, and the current progression of SARS-CoV-2 RNA, antibodies, antigens, and virus detection. Further, we discuss the reasons for the observed false-negative and false-positive RNA and antibody detection results in practical clinical applications. Finally, we provide a review of the biosensors which hold promising potential for point-of-care detection of COVID-19 patients. This review thereby provides general guidelines for both scientists in the biosensing research community and for those in the biosensor industry to develop a highly sensitive and accurate point-of-care COVID-19 detection system, which would be of enormous benefit for controlling the current COVID-19 pandemic.

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Section: Potential Biosensors For Point-of-care Sars-cov-2 Detectionmentioning

confidence: 99%

Section: The Unmet Problem For Sars-cov-2 Detectionmentioning

confidence: 99%

Detection of COVID-19: A review of the current literature and future perspectives

Liu

Wang

et al. 2020

Biosensors and Bioelectronics

353

309

View full text Add to dashboard Cite

show abstract

“…Likewise, a stir bar coated with AuNPs and combined with a microchip device has been shown to be effective in restoring DNA multi-arm link recovery for signal transduction and amplification purposes in the rapid detection of antibiotics [ 64 ]. In addition, some microfluidic pre-concentration/amplification techniques have been applied to biological samples such as isotachophoresis (ITP) [ 120 , 121 , 122 , 123 , 124 , 125 , 126 ], ion concentration polarization (ICP) [ 127 , 128 , 129 , 130 , 131 , 132 ], electrokinetic stacking (EKS) [ 133 , 134 , 135 , 136 , 137 ], field amplification stacking (FAS) [ 138 , 139 , 140 , 141 ], and multiplex electroanalytical techniques [ 142 , 143 ]. Although it has not been applied to milk analysis, it is also an important reference for future research.…”

Section: Discussionmentioning

confidence: 99%

Recent Advances in Microfluidic Devices for Contamination Detection and Quality Inspection of Milk

Lee

Kung

et al. 2021

Micromachines

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Milk is a necessity for human life. However, it is susceptible to contamination and adulteration. Microfluidic analysis devices have attracted significant attention for the high-throughput quality inspection and contaminant analysis of milk samples in recent years. This review describes the major proposals presented in the literature for the pretreatment, contaminant detection, and quality inspection of milk samples using microfluidic lab-on-a-chip and lab-on-paper platforms in the past five years. The review focuses on the sample separation, sample extraction, and sample preconcentration/amplification steps of the pretreatment process and the determination of aflatoxins, antibiotics, drugs, melamine, and foodborne pathogens in the detection process. Recent proposals for the general quality inspection of milk samples, including the viscosity and presence of adulteration, are also discussed. The review concludes with a brief perspective on the challenges facing the future development of microfluidic devices for the analysis of milk samples in the coming years.

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“…Combined with the assistance of an electric field with a membrane, the membrane-based EAE can further improve extraction efficiency. For example, Ouyang and Han [42] fabricated a cation-selective Nafion membrane strip crossing parallel straight channels in their NA extraction microchip. Once loading cell lysate and applying an electric field on these channels, the membrane-based EAE can concentrate DNA and remove proteins and metabolites.…”

Section: Microfluidic Field-assisted Extraction Techniquesmentioning

confidence: 99%

Recent progress of microfluidic sample preparation techniques

Xia

2023

J of Separation Science

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Sample preparation turns out to be one of the important procedures in complex sample analysis by affecting the accuracy, selectivity, and sensitivity of analytical results. However, the majority of the conventional sample preparation techniques still suffer from time‐consuming and labor‐intensive operations. These shortcomings can be addressed by reforming the sample preparation process in a microfluidic manner. Inheriting the advantages of rapid, high efficiency, low consumption, and easy integration, microfluidic sample preparation techniques receive increasing attention, including microfluidic phases separation, microfluidic field‐assisted extraction, microfluidic membrane separation, and microfluidic chemical conversion. This review overviews the progress of microfluidic sample preparation techniques in the last 3 years based on more than 100 references, we highlight the implementation of typical sample preparation methods in the formats of microfluidics. Furthermore, the challenges and outlooks of the application of microfluidic sample preparation techniques are discussed.

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One‐Step Nucleic Acid Purification and Noise‐Resistant Polymerase Chain Reaction by Electrokinetic Concentration for Ultralow‐Abundance Nucleic Acid Detection

Abstract: Supporting information for this article is given via a link at the end of the document.

Cited by 5 publications

References 61 publications

Detection of COVID-19: A review of the current literature and future perspectives

Detection of COVID-19: A review of the current literature and future perspectives

Recent Advances in Microfluidic Devices for Contamination Detection and Quality Inspection of Milk

Recent progress of microfluidic sample preparation techniques

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