2023
DOI: 10.1021/acs.jafc.3c06731
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One-Step Platform for Maduramicin and Salinomycin Detection Based on Bispecific Monoclonal Antibody and Interpretation of Molecular Recognition Mechanism

Jingjie Huang,
Yingxian Chen,
Zhixuan Sun
et al.

Abstract: Maduramicin (MAD) and salinomycin (SAL) are the widely used poly­(ether ionophore) antibiotics to control coccidiosis in animals. Due to their strong cytotoxicity, strict control over their dosage and residue in animal food is necessary. To improve the detection efficiency of the existing single-residue detection methods, a tetraploid tumor hybrid system was constructed using drug mutagenesis, and the bispecific monoclonal antibody (BsMAb) against MAD and SAL was obtained by hybridization-hybridoma technology.… Show more

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