One step pre-hardening micro propagation of Bambusa balcooa Roxb

Patel, Beena; Gami, Bharat; Patel, Nisha; Bariya., Vipul

doi:10.5455/jp.2015-06-02

Cited by 12 publications

(7 citation statements)

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“…8.2±2.4 shoots per explant and highest shoot length 3.8±0.4 were recorded. Similar observation were also made by Sharma et al, 2011 andPatel et al, 2015. Mature buds were then transferred in multiplication media for 2-3 weeks at controlled conditions. Best shoot multiplication was found in media containing BA 1.5 mg/l.…”

Section: Resultssupporting

confidence: 54%

See 1 more Smart Citation

In-vitro Regeneration of Bambusa balcooa (Bamboo) through Nodal Segments

Ansari¹,

Gupta²,

Soni³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Section: Resultssupporting

confidence: 54%

“…Leaf sheath tissues and dead parts of the upper internodes were removed through scalpel (Sharma et al, 2011 andPatel et al, 2015) Surface sterilization of ex-plant…”

Section: Collection Of Ex-plantmentioning

confidence: 99%

In-vitro Regeneration of Bambusa balcooa (Bamboo) through Nodal Segments

Ansari¹,

Gupta²,

Soni³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…cattaniae (Marijana et al, 2012), Bambusa balcooa Roxb. (Beena et al, 2015), Cicer arietinum (Sujatha et al, 2007), Malus domestica (Bommineni et al, 2001), and Aloe vera (Dwivedi et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

RAPID ONE STEP PROTOCOL FOR THE in vitro MICRO PROPAGATION OF Morus multicaulis VAR. GOSHOERAMI, AN ELITE MULBERRY VARIETY OF TEMPERATE REGION

Rohela¹,

Shabnam²,

Shukla³

et al. 2018

JEBAS

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Morus multicaulis cv. Goshoerami is the leading mulberry variety for silkworm rearing under temperate climatic conditions of Jammu and Kashmir, India. However, the propagation of this popular mulberry variety has always remained a point of contention due to its poor rooting response through stem cuttings. It normally takes 4 to 5 years for raising the saplings of this variety through conventional root grafting techniques. Therefore, for quick propagation of this poor rooting popular mulberry variety, a one step in vitro protocol was developed by culturing nodal explants from 2 year old plants on Murashige & Skoog (MS) media supplemented with individual as well as combination of phytohormones. The maximum shoot bud proliferation (6.3± 0.71 in cm) and rooting (14.7± 0.53 in cm) was observed when nodal explants were cultured on the combinational media of BAP (1 mg/L) and IBA (1 mg/L) after 14 days of culture. These in vitro raised plantlets were hardened by using the sterile soil and vermiculite in 2:1 ratio. Only 25 days were required for the micro propagation and hardening of raised plantlets of Goshoerami through this single step protocol. The hardened plantlets were successfully established in the field with 83% survival rate. The developed one step protocol can be used efficiently for the mass propagation of this elite mulberry variety throughout the year with in short span of 25 days.

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“…Explants collected during February-March and September-October showed maximum bud break [48]. It was reported that the rainy season was better for bud break in Dendrocalamus strictus [49] Bambusa tulda [40] Gigantochloa atroviolacea [50] with the high rate of contamination [40,51,52]. The explants collected from November to January (winter months) produced only 35-45% bud break response during the culture of Bambusa vulagaris by [53].…”

Section: Collection Of Explantsmentioning

confidence: 99%

“…These three hormones were more suitable for rooting in D. asper [84, 91,92,93,94, 97, and 98]. Clusters of 3-5 shoots were effective for transferring into rooting medium [29,51,58,96,99,123,124]. (2012b) have observed that 100% rooting in D. asper by using a combination of IBA and NAA.…”

Section: Rootingmentioning

confidence: 99%

Regeneration Technique of Bamboo Species through Nodal Segments: A Review

2020

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Micropropagation is an alternative technique to propagate at large scale plants to meet global plant demand. Various researchers have worked on the micropropagation technique to regenerate bamboo species by using nodal segments from years. Contamination, browning, necrosis, and acclimatization with physiological stress are the extreme problems of the micropropagation technique. But, many numbers of papers have been published on micropropagation of the bamboo species through nodal segments as explants. The proliferation of the bamboo shoots is dependent on the season of collection, size of explants, the position of explants, diversity of plants, concentration and combination of plant growth regulators, most adequate culture medium, environmental condition of the equipment, handling, and individual species. Bamboo is a monocarpic fast-growing, tall perennial grass and having the high potential to generate economic and social benefits. It helps to maintain land patterns and control soil erosion. The long life cycle of the bamboo produces a huge amount of seeds but unfortunately, mostly, they are non-viable. So, bamboos are propagated from vegetative by cutting and air layering. However, these methods are only for a small scale and they also tend to destroy large mother plant stocks and difficult to be transported. So, the in vitro propagation technique is useful to obtain large progenies from desired genotypes. Mostly, BAP and TDZ growth hormones are widely used for shoot multiplication and IBA, NAA and IAA are used for root initiation as per developed protocols in tissue culture for large scale production. This review intends to explore an overview of the recent literature reports to summarize the importance of micropropagation by using nodal segments of bamboo species and factors influencing it.

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One step pre-hardening micro propagation of Bambusa balcooa Roxb

Cited by 12 publications

References 0 publications

In-vitro Regeneration of Bambusa balcooa (Bamboo) through Nodal Segments

In-vitro Regeneration of Bambusa balcooa (Bamboo) through Nodal Segments

RAPID ONE STEP PROTOCOL FOR THE in vitro MICRO PROPAGATION OF Morus multicaulis VAR. GOSHOERAMI, AN ELITE MULBERRY VARIETY OF TEMPERATE REGION

Regeneration Technique of Bamboo Species through Nodal Segments: A Review

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