One-step self-assembly of quantum dot-based spherical nucleic acid nanostructure for accurate monitoring of long noncoding RNA MALAT1 in living cells and tissues

Zhang, Qian; Zhao, Ran; Zhang, Yan; Zou, Xiaoran; Zhang, Chunyang

doi:10.1016/j.cej.2023.144021

Cited by 7 publications

(3 citation statements)

References 59 publications

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“…QDs with excellent optical properties were selected as donors. 27–30 For our immunoassay, we selected QD 545 as the FRETdonor and AFT as the acceptor phenol. This choice was made because the emission spectrum of QD 545 overlaps well with the absorbance spectrum of AFT, and their emission spectra do not interfere with each other (Fig.…”

Section: Resultsmentioning

confidence: 99%

Homogeneous immunoassay utilizing fluorescence resonance energy transfer from quantum dots to tyramide dyes deposited on full immunocomplexes

Xu,

Liu,

Zong

et al. 2023

Analyst

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Section: Resultsmentioning

confidence: 99%

Homogeneous immunoassay utilizing fluorescence resonance energy transfer from quantum dots to tyramide dyes deposited on full immunocomplexes

Xu,

Liu,

Zong

et al. 2023

Analyst

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“…However, these methods often involve tedious separation/washing steps, the preparation of modified electrodes and nanomaterials synthesis, and low sensitivity, which limits the practical applications, especially in the detection of trace lncRNAs. To improve the sensitivity, different signal amplification strategies have been introduced in lncRNA assays, including recombinase polymerase amplification, exponential amplification reaction, rolling circle amplification, primer exchange reaction, and duplex-specific nuclease (DSN)-aided signal amplification . Despite the improved sensitivity, these strategies often utilize ssDNA reporters, ,, molecular beacon, SYBR dyes, and Thioflavin T (ThT) , for signal output.…”

Section: Introductionmentioning

confidence: 99%

“…To improve the sensitivity, different signal amplification strategies have been introduced in lncRNA assays, including recombinase polymerase amplification, exponential amplification reaction, rolling circle amplification, primer exchange reaction, and duplex-specific nuclease (DSN)-aided signal amplification . Despite the improved sensitivity, these strategies often utilize ssDNA reporters, ,, molecular beacon, SYBR dyes, and Thioflavin T (ThT) , for signal output. Fluorophore- and quencher-labeled probes may suffer from incomplete fluorescence quenching/self-quenching-induced false positives and high cost .…”

Section: Introductionmentioning

confidence: 99%

Development of a DNAzyme-Driven Fluorescent Light-Up Aptasensor for Label-Free Detection of Multiple lncRNAs

Yang,

Han,

Zhang

et al. 2024

Anal. Chem.

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Long noncoding RNAs (lncRNAs) act as the dynamic regulatory molecules that control the expression of genes and affect numerous biological processes, and their dysregulation is associated with tumor progression. Herein, we develop a fluorescent light-up aptasensor to simultaneously measure multiple lncRNAs in living cells and breast tissue samples based on the DNAzyme-mediated cleavage reaction and transcription-driven synthesis of light-up aptamers. When target lncRNAs are present, they can be recognized by template probes to form the active DNAzyme structures, initiating the T4 PNK-catalyzed dephosphorylationtriggered extension reaction to generate double-strand DNAs with the T7 promoter sequences. The corresponding T7 promoters can initiate the transcription amplification catalyzed by the T7 RNA polymerase to generate abundant Broccoli aptamers and malachite green aptamers, which can bind DFHBI-1T and MG to generate strong fluorescence signals. Taking advantage of the good selectivity of DNAzyme-mediated cleavage of lncRNAs, high amplification efficiency of T7 transcriptiondriven amplification reaction, and bright fluorescence of the RNA aptamer-fluorophore complex, this method exhibits high sensitivity with a detection limit of 21.4 aM for lncRNA HOTAIR and 18.47 aM for lncRNA MALAT1, and it can accurately measure multiple lncRNAs in both tumor cell lines and breast tissue samples, providing a powerful paradigm for biomedical research and early clinic diagnostics.

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Construction of single-molecule counting-based biosensors for DNA-modifying enzymes: A review

Zhang,

Hu,

et al. 2024

Analytica Chimica Acta

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One-step self-assembly of quantum dot-based spherical nucleic acid nanostructure for accurate monitoring of long noncoding RNA MALAT1 in living cells and tissues

Cited by 7 publications

References 59 publications

Homogeneous immunoassay utilizing fluorescence resonance energy transfer from quantum dots to tyramide dyes deposited on full immunocomplexes

Homogeneous immunoassay utilizing fluorescence resonance energy transfer from quantum dots to tyramide dyes deposited on full immunocomplexes

Development of a DNAzyme-Driven Fluorescent Light-Up Aptasensor for Label-Free Detection of Multiple lncRNAs

Construction of single-molecule counting-based biosensors for DNA-modifying enzymes: A review

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