Onosma ambigens: Phytochemical composition, antioxidant and enzyme inhibitory activity

Sarıkürkçü, Cengiz; Sahinler, Saliha Seyma; Ceylan, Olcay; Tepe, Bektaş

doi:10.1016/j.indcrop.2020.112651

Cited by 18 publications

(33 citation statements)

References 40 publications

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Section: Resultsmentioning

confidence: 55%

“…Rindera species have not been assayed for their phenolic and flavonoid content to the best of our knowledge. In this study, the total phenolic content (TPC) ( Table 3) of the R. graeca methanolic extract is significantly high, at 66.5 ± 1.6 GAE mg/g compared to other Boraginaceae pants, while the total flavonoid content (TFC) ( Table 3) is rather low at 9.7 ± 0.1 compared to other plants of the same family [32][33][34][35]. The DPPH and ABTS assays on the R. graeca methanolic extract (Table 3) showed significant inhibition of the free radicals (near 100% inhibition at higher doses for the ABTS assay) and, therefore, a significant antioxidative profile.…”

Section: Total Phenolic/flavonoid Content and Antioxidant Activity Rementioning

confidence: 53%

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Rindera graeca (Boraginaceae) Phytochemical Profile and Biological Activities

et al. 2020

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Rindera graeca is a Greek endemic plant of the Boraginaceae family which has never been studied before. Consequently, this study attempted to phytochemically examine the aerial parts of this species. Nine phenolic secondary metabolites were identified, consisting of seven caffeic acid derivatives and two flavonol glucosides, namely rutin and quercetin-3-rutinoside-7-rhamnoside. These flavonoids, together with rosmarinic acid, were isolated via column chromatography and structurally determined through spectral analysis. Quercetin-3-rutinoside-7-rhamnoside is an unusual triglycoside, which is identified for the first time in Rindera genus and among Boraginaceae plants. This metabolite was further examined with thermal analysis and its 3D structure was simulated, revealing some intriguing information on its interaction with biological membrane models, which might have potential applications in microcirculation-related conditions. R. graeca was also analyzed for its pyrrolizidine alkaloids content, and it was found to contain echinatine together with echinatine N-oxide and rinderine N-oxide. Additionally, the total phenolic and flavonoid contents of R. graeca methanol extract were determined, along with free radical inhibition assays. High total phenolic content and almost complete inhibition at experimental doses at the free radical assays indicate a potent antioxidant profile for this plant. Overall, through phytochemical analysis and biological activity assays, insight was gained on an endemic Greek species of the little-studied Rindera genus, while its potential for further applications has been assessed.

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Section: Resultsmentioning

confidence: 55%

Section: Total Phenolic/flavonoid Content and Antioxidant Activity Rementioning

confidence: 53%

Rindera graeca (Boraginaceae) Phytochemical Profile and Biological Activities

et al. 2020

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“…In previous studies, it was reported that some Onosma and Anthemis species were to be rich in apigenin 7-glucoside showing significant antioxidant activity [ 5 , 26 , 29 , 30 ]. There were also some studies in the literature published by other research groups that this compound may positively affect antioxidant activity.…”

Section: Discussionmentioning

confidence: 99%

“…As with apigenin 7-glucoside, previous studies also revealed that various Onosma species were also rich in luteolin 7-glucoside exhibiting significant antioxidant activities [ 5 , 26 , 29 , 30 ]. Additionally, it has been reported that the free radical scavenging and ferrous ion chelating activities of the hydro-alcoholic extract obtained from Fraxinus angustifolia were quite successful compared to vitamin E, even acting as an effective chain-breaking antioxidant against lipoperoxyl radicals.…”

Section: Discussionmentioning

confidence: 99%

“…However, the amount of this substance can decrease dramatically, especially in the elderly individuals, due to the hydrolysis by cholinesterases (ChEs), which are responsible for the regulation of the amount of ACh [ 22 ]. Therefore, inhibition of ChEs is a useful strategy in these individuals in order to prevent the decrease in the amount of neurotransmitters and to stabilize cognitive functions [ 5 , 23 , 24 , 25 , 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

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Chemical Composition, Antioxidant and Enzyme Inhibitory Activities of Onosma bourgaei and Onosma trachytricha and in Silico Molecular Docking Analysis of Dominant Compounds

İstifli

2021

Molecules

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The aim of this study was to investigate the chemical composition, antioxidant and enzyme inhibitory activities of methanol (MeOH) extracts from Onosma bourgaei (Boiss.) and O. trachytricha (Boiss.). In addition, the interactions between phytochemicals found in extracts in high amounts and the target enzymes in question were revealed at the molecular scale by performing in silico molecular docking simulations. While the total amount of flavonoid compounds was higher in O. bourgaei, O. trachytricha was richer in phenolics. Chromatographic analysis showed that the major compounds of the extracts were luteolin 7-glucoside, apigenin 7-glucoside and rosmarinic acid. With the exception of the ferrous ion chelating assay, O. trachytricha exhibited higher antioxidant activity than O. bourgaei. O. bourgaei exhibited also slightly higher activity on digestive enzymes. The inhibitory activities of the Onosma species on tyrosinase were almost equal. In addition, the inhibitory activities of the extracts on acetylcholinesterase (AChE) were stronger than the activity on butyrylcholinesterase (BChE). Molecular docking simulations revealed that luteolin 7-glucoside and apigenin 7-glucoside have particularly strong binding affinities against ChEs, tyrosinase, α-amylase and α-glucosidase when compared with co-crystallized inhibitors. Therefore, it was concluded that the compounds in question could act as effective inhibitors on cholinesterases, tyrosinase and digestive enzymes.

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Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents

Kostić

Mačukanović-Jocić

Milinčić

et al. 2022

Chemistry & Biodiversity

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The current study aimed to phytochemically characterize (including a detailed phenolic profile) two endemic Balkan's species (Hieracium waldsteinii and Onosma stellulata) and determine their possible application as a source of natural antioxidant and antimicrobial agents. The main phenolic compound in both species (in all examined parts) was chlorogenic acid. Eriodictyol, genistein and naringenin were quantified only in H. waldsteinii while isorhamnetin‐3‐O‐rutinoside and sinapic acid were characteristic for O. stellulata. The highest antioxidant activity (98 mg AAE/g dry weight for TAC assay) was ascribed to the flower extract of H. waldsteinii while the lowest results (∼4.3 mg AAE/g dry weight for FRP assay) were exhibited by the extracts obtained from the plant's stem. Antimicrobial assays showed moderate antibacterial, i. e., moderate/strong activity against several tested fungi (in particular Trichoderma viride). Correlation analysis revealed strong positive connection between phenolic compounds and reducing power of extracts as well as between total phenolic and flavonoid content and the obtained minimal inhibitory concentration recorded in antibacterial assays.

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Onosma ambigens: Phytochemical composition, antioxidant and enzyme inhibitory activity

Cited by 18 publications

References 40 publications

Rindera graeca (Boraginaceae) Phytochemical Profile and Biological Activities

Rindera graeca (Boraginaceae) Phytochemical Profile and Biological Activities

Chemical Composition, Antioxidant and Enzyme Inhibitory Activities of Onosma bourgaei and Onosma trachytricha and in Silico Molecular Docking Analysis of Dominant Compounds

Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents

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