Ontogenic development of the intestinal enzymes of cultured Nile tilapia, Oreochromis niloticus L.

Tengjaroenkul, Bundit; Smith, Bonnie J.; Smith, Stephen A.; Chatreewongsin, Urai

doi:10.1016/s0044-8486(01)00888-2

Cited by 49 publications

(41 citation statements)

References 25 publications

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“…On the other hand, exopeptidases such as carboxypeptidase A and leucine aminopeptidase were determined, which showed activity from the moment of larvae hatching, similarly to what was found in C. urophthalmus (López-Ramírez et al 2011), while leucine aminopeptidase early activity was described in species such as P. splendida (Uscanga-Martínez et al 2011) and Nile tilapia, Oreochromis niloticus (Tengjaroenkul et al 2002). Instead in species such as S. ocellatus (Lazo et al 2007); P. maculatofasciatus (Á lvarez-González et al 2008); C. undecimalis (Jiménez-Martínez et al 2012) at the beginning, a low leucine aminopeptidase activity is detected, increasing with time or with feed changes.…”

Section: Discussionsupporting

confidence: 63%

Changes on digestive enzymes during initial ontogeny in the three-spot cichlid Cichlasoma trimaculatum

Toledo-Solís

Uscanga‐Martínez

Guerrero‐Zárate

et al. 2014

Fish Physiol Biochem

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A study was performed in order to understand the development of digestive enzymes during initial ontogeny of Cichlasoma trimaculatum, for which the activity of acidic and alkaline proteases, lipases, amylases and phosphatases was determined by means of biochemical and electrophoretic analysis. Our results showed that the activity of alkaline proteases, trypsin and chymotrypsin is present from day 6 after hatching (dah) during exogenous feeding with Artemia nauplii. The activities of carboxypeptidase A and leucine aminopeptidase are present from the first days, increasing at 6 dah and reaching their maximum activity at 9 dah while acid protease activity started at 9 dah. Furthermore, the lipase activity is detected on 6 dah and keeps increasing and decreasing on 17 dah. Amylase activity is detected on 3 dah, presenting fluctuations until 45 dah, where it reaches its maximum activity. Acid and alkaline phosphatases are detected from 3 dah and reach a maximum activity between 13 and 19 dah. The SDS-PAGE electrophoresis revealed six types of bands in the alkaline proteases, with molecular weight between 113.4 and 20.4 kDa. First three bands appear on 6 dah, but it is until 11 dah when all isoforms appear. Based on these results, it is considered that this species completes its digestive enzymatic machinery from day 9 after hatching, therefore is recommended to perform the transition from live feed to inert feed at 15 dah.

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Section: Discussionsupporting

confidence: 63%

Changes on digestive enzymes during initial ontogeny in the three-spot cichlid Cichlasoma trimaculatum

Toledo-Solís

Uscanga‐Martínez

Guerrero‐Zárate

et al. 2014

Fish Physiol Biochem

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“…It seems that bullfrogs need time, in the present case two weeks, to optimize lipase activity. This delayed appearance of lipase activity was also found for Nile tilapia, for which the activity was first detected in the brush border of the enterocytes, three days after hatch (Tengjaroenkul et al, 2002).…”

Section: Methodssupporting

confidence: 69%

Enzymatic activity of lipase in post-metamorphic phase bullfrogs

Braga

Oliveira²,

Lima³

et al. 2006

Sci. agric. (Piracicaba, Braz.)

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The knowledge of the digestive system of bullfrogs is an important step for the determination of their nutritional requirements throughout growth phases. With the objective of evaluating the enzymatic activity of lipase in the intestinal content of bullfrogs (Rana catesbeiana Shaw, 1802), 100 animals with median weight of 3.6 g were distributed in stalls under controlled temperature and photoperiod. The frogs, selected at the post-metamorphic phase, received commercial extruded diet ad libitum throughout the 87-day experiment. The collections of the intestinal content were performed by the desensitization of the frogs in ice and water at 0ºC and subsequent isolation of the small intestine. Determination of lipase activity was performed with a commercial enzymatic kit (Lipase-Bioclin, MG, Brazil), first measured in samples taken at day three (3.46 UI). During the initial phase the bullfrog possesses low lipase hydrolysis capacity was found, having a specific activity of 217 UI mg -1 . In the subsequent period both lipase activity and specific lipase activity continuously increased. Lipase activity as a function of bullfrog weight fell after day twenty and reached 0.33 UI g -1 , for frogs of medium weight (179 g). Feed for bullfrogs at the post-metamorphic phase weighing more than 10 g can have larger amounts of ingredients containg lipids, due to the increased digestive capacity of these frogs. Key words: digestive enzyme, frog culture, specific activity ATIVIDADE ENZIMÁTICA DA LIPASE EM RÃ-TOURO NA FASE PÓS-METAMÓRFICA RESUMO: O conhecimento do sistema digestório da rã-touro é um passo importante para a determinação de sua exigência nutricional nas diferentes fases de crescimento. Com o objetivo de avaliar a atividade enzimática da lipase no conteúdo intestinal da rã-touro (Rana catesbeiana Shaw, 1802), 100 animais com peso médio de 3,6 g foram distribuídos em baias-teste com temperatura e fotoperíodo controlados. As rãs, selecionadas na fase pos-metamórfica receberam ração extruzada comercial ad libitum. Durante 87 dias de experimento, 29 coletas (87 rãs) foram feitas em intervalos que variaram de um a oito dias. As rãs foram insensibilizadas em água e gelo a 0ºC para subseqüente isolamento do intestino delgado e retirada do conteúdo intestinal. Para determinação da atividade da lipase foram usados conjuntos enzimáticos da BIOCLIN. A atividade da lipase foi registrada no terceiro dia (3,46 UI). Na fase inicial, a rã-touro possui baixa capacidade de hidrólise para a lipase que teve atividade específica de 217 UI mg -1 . No período subseqüente, observou-se aumento contínuo da atividade e da atividade específica da lipase. A atividade da lipase em função do peso da rã-touro depois do 20 o dia apresentou redução contínua, alcançando valor de 0,33 UI g -1 , registrado para rãs com peso médio de 179 g. As rações para rã-touro com peso superior a 10 g podem conter quantidades maiores de ingredientes que contenham lipídos, devido ao aumento da capacidade da digestão. Palavras-chave: atividade específica, enzi...

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“…Crustacean larval digestive physiology is a reflection of diet and feeding strategy , Le Vay et al 2001, and ontogenetic changes in digestive enzyme expression during larval development may be used to identify natural developmental feeding transitions (Hammer et al 2000). Adjusting larval artificial feed formulations to coincide with any natural feeding transitions would result in greater productivity by improving the health, maximising growth, and reducing waste of feed in an aquaculture setting (Tengjaroenkul et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Digestive enzyme profiles of spiny lobster Jasus edwardsii phyllosoma larvae

Johnston¹,

Ritar²,

Thomas³

et al. 2004

Mar. Ecol. Prog. Ser.

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Digestive enzyme activities of cultured (Stage I to VI) and wild (Stage V to XI) phyllosoma larvae of the spiny lobster Jasus edwardsii were investigated over progressive stages of development to provide an indication of their digestive capabilities and nutritional requirements and to help identify the characteristics of their natural prey. Protease, trypsin, amylase, α-glucosidase, chitinase and lipase were detected in all cultured and wild specimens, suggesting phyllosoma can readily digest dietary protein, lipid and carbohydrate, including chitin at all stages of development. Protease and lipase activities were considerably higher than amylase and α-glucosidase, indicating that dietary protein and lipid is more important than carbohydrate and thus suggests a carnivorous diet. Total digestive enzyme activities (Units larva -1 , units defined as the amount of enzyme that catalysed the release of 1 µmole of product per minute) increased significantly with larval development, reflecting the considerable increase in digestive capacity that is required to meet the metabolic requirements of increasing larval body mass. Relatively constant specific enzyme activity (Units mg -1 ) in cultured larvae fed the same diet suggests that specific activity variations evident in wild larvae may reflect changes in natural diet or feeding abilities. A large increase in protease, trypsin and amylase specific activity between wild phyllosoma Stages VI and VII may be driven by an increase in food availability or processing efficiency that precedes a large increase in phyllosoma size. Enzyme profiles for both cultured and wild J. edwardsii phyllosoma suggest that spiny lobster phyllosoma are capable of digesting a wide range of zooplankton prey, but they make best use of dietary items that are high in protein and lipid.

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Ontogenic development of the intestinal enzymes of cultured Nile tilapia, Oreochromis niloticus L.

Cited by 49 publications

References 25 publications

Changes on digestive enzymes during initial ontogeny in the three-spot cichlid Cichlasoma trimaculatum

Changes on digestive enzymes during initial ontogeny in the three-spot cichlid Cichlasoma trimaculatum

Enzymatic activity of lipase in post-metamorphic phase bullfrogs

Digestive enzyme profiles of spiny lobster Jasus edwardsii phyllosoma larvae

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