Ontogeny of the neurotensin‐containing neuron system of the rat: Immunohistochemical analysis. I. Forebrain and diencephalon

Hara, Yoshinobu; Shiosaka, Sadao; Senba, Emiko; Sakanaka, Masahiro; Inagaki, Shinobu; Takagi, Hiroshi; Kawai, Yoshinori; Takatsuki, Kenichi; Matsuzaki, Takashi; Tohyama, Masaya

doi:10.1002/cne.902080207

Cited by 126 publications

(30 citation statements)

References 40 publications

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“…There was intense labeling of the CA1 region of hippocampus ( Fig. 2 C and D) that was unexpected in view of the reported absence of NT-immunoreactive cell bodies in this region (8,9,(15)(16)(17). This labeling occurred at all septotemporal levels of the hippocampus.…”

Section: Resultsmentioning

confidence: 56%

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Distribution of neurotensin/neuromedin N mRNA in rat forebrain: unexpected abundance in hippocampus and subiculum.

Alexander

Miller

Dorsa

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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We have used in situ hybridization to determine the regional distribution of mRNA encoding the neurotensin/neuromedin N (NT/N) precursor in the forebrain of the adult male rat. Cells containing NT/N mRNA are widely distributed in the forebrain. These areas include the septum, bed nucleus of the stria terminalis, preoptic area, hypothalamus, amygdala, accumbens nucleus, caudate-putamen, and piriform and retrosplenial cortex. In general, the regional distribution of NT/N mRNA corresponds to the previously determined distribution of neurotensin-immunoreactive cell bodies; however, several notable exceptions were observed.The most striking difference occurs specifically in the CA1 region of the hippocampus, where intense labeling is associated with the pyramidal cell layer despite the reported absence of neurotensin-immunoreactive cells in this region. Analysis of microdissected tissue by S1 nuclease protection assay confirmed the abundance of authentic NT/N mRNA in CAL. A second major discrepancy between NT/N mRNA abundance and neurotensin-immunoreactivity occurs in the intensely labeled subiculum, a region that contains only scattered neurotensin-immunoreactive cells in the adult. These results suggest that, in specific regions of the forebrain, NT/N precursor is processed to yield products other than neurotensin. In addition, these results provide an anatomical basis for studying the physiological regulation of NT/N mRNA levels in the forebrain.Neurotensin (NT), a tridecapeptide with widespread distribution in the central nervous system and peripheral tissues of mammals, is one of a family of structurally related peptides with members manifesting diverse biological activities (1). The structure of the rat gene encoding NT has recently been determined (2), and the general features of the predicted NT precursor for the rat are the same as those of the predicted precursors for the dog (3) and cow (2). In each species, a precursor protein of 169-170 amino acid residues encodes both NT and neuromedin N, a peptide similar in structure to NT (4). A comparison of the sequences of the complete neurotensin/neuromedin N (NT/N) precursor from rat, dog, and cow reveals a high degree of conservation. This finding is consistent with the idea that the precursor may be processed to yield biologically active peptides that are as yet unknown.There is increasing evidence that NT functions as a neuroregulatory substance in the mammalian central nervous system (1, 5). Effects produced by NT administration in the central nervous system include hypothermia, hypotension, and antinociception. NT has also been implicated in the central regulation of anterior pituitary function (6, 7). NTimmunoreactive cell bodies are most numerous in the forebrain, with widespread distribution in limbic regions such as the septum, preoptic area, hypothalamus, and amygdala (8).Other forebrain regions, such as the dorsal subiculum, contain many NT-immunoreactive cell bodies during early stages of development, but the number of neurons with detectable...

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Section: Resultsmentioning

confidence: 56%

“…Other forebrain regions, such as the dorsal subiculum, contain many NT-immunoreactive cell bodies during early stages of development, but the number of neurons with detectable NT-like immunoreactivity decreases dramatically by adulthood (9).…”

mentioning

confidence: 99%

Distribution of neurotensin/neuromedin N mRNA in rat forebrain: unexpected abundance in hippocampus and subiculum.

Alexander

Miller

Dorsa

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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“…Both the peptide neurotensin and its receptor are highly expressed during early postnatal cortical development (Hara et al, 1982;Palacios et al, 1988;Lepee-Lorgeoux et al, 1999). Experiments were performed using either neurotensin 8-13 (an active fragment of the tridecapeptide neurotensin, made from amino acids 8 -13) or the neurotensin-like peptide Neuromedin-N.…”

Section: A Region In the Mediodorsal Cortex Exhibits Wave-like Largesmentioning

confidence: 99%

Gq/11-Induced and Spontaneous Waves of Coordinated Network Activation in Developing Frontal Cortex

Calderon¹,

Leverkova²,

Peinado³

2005

J. Neurosci.

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Repeated episodes of spontaneous large-scale neuronal bursting and calcium influx in the developing brain can potentially affect such fundamental processes as circuit formation and gene expression. Between postnatal day 3 (P3) and P7, the immature cortex can express one such form of activation whereby a wave of neuronal activity propagates through cortical networks, generating massive calcium influx. We previously showed that this activity could be triggered by brief stimulation of muscarinic receptors. Here, we show, by monitoring large cortical areas at low magnification, that although all areas respond to muscarinic agonists to some extent, only some areas are likely to generate the coordinated wave-like activation. The waves can be triggered repeatedly in frontal areas where, as we also show, waves occur spontaneously at a low frequency. In parietal and occipital areas, no such waves are seen. This selectivity may be related in part to differences in the cortical distribution of dopaminergic signaling, because we find that activation of dopamine receptors enables the response. Because M1 muscarinic receptors are typically coupled with G-␣ q/11, we investigated whether other receptors known to couple with this G-protein (group I glutamate metabotropic receptors, neurotensin type 1) could similarly elicit wave-like activation in responsive cortical areas. Our results suggest that multiple neurotransmitter systems can enable this form of activation in the frontal cortex. The findings suggest that a poorly recognized, developmentally regulated form of strong network activation found predominantly in the frontal cortex could potentially exert a profound influence on brain development.

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“…6E, F) and n. medialis thalami pars lateralis, ventral tegmental nucleus of Gudden and n. centralis superior (Fig. 7) (2, 11), SPI in the cerebellum (6), SOMI in the cerebellum (8), some brainstem nuclei (21)(22)(23) and spinal cord (20), and NTI in some other brain regions (4,12). At present the reason for this decrease is not clear.…”

mentioning

confidence: 93%

Ontogeny of neuropeptides in the rat brain: An immunohistochemical analysis.

Tohyama

1985

Acta Histochem. Cytochem

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Ontogeny of the neurotensin‐containing neuron system of the rat: Immunohistochemical analysis. I. Forebrain and diencephalon

Cited by 126 publications

References 40 publications

Distribution of neurotensin/neuromedin N mRNA in rat forebrain: unexpected abundance in hippocampus and subiculum.

Distribution of neurotensin/neuromedin N mRNA in rat forebrain: unexpected abundance in hippocampus and subiculum.

Gq/11-Induced and Spontaneous Waves of Coordinated Network Activation in Developing Frontal Cortex

Ontogeny of neuropeptides in the rat brain: An immunohistochemical analysis.

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