Oocyte activation during round spermatid injection: state of the art

Tao, Yong

doi:10.1016/j.rbmo.2022.03.024

Cited by 6 publications

(5 citation statements)

References 77 publications

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“…The problem of male infertility in patients with globozoospermia can be solved by the procedure of intracytoplasmic sperm injection (ICSI), with possible options of assisted oocyte activation, in most cases performed by the addition of calcium ionophore, which significantly improves the fertilization rate ( Karaca et al, 2015 ; Huang et al, 2022 ; Tao, 2022 ). Obviously, the future of genetic diagnosis, reproductive evaluation and the treatment of globozoospermia, as well as a number of other syndromic, systemic spermiopathologies, lies in the search and analysis of genes leading to these forms of male infertility.…”

Section: Discussionmentioning

confidence: 99%

Atypical structure of the nuclear membrane, distribution of nuclear pores and lamin B1 in spermatozoa of patients with complete and partial globozoospermia

Bragina,

Kurchashova,

Suhomlinova

et al. 2024

Front. Genet.

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Globozoospermia is a form of male infertility characterized by spermatozoa with spherical heads lacking acrosomes. The aim of this study was to evaluate ultrastructural and molecular defects in different types of globozoospermia. Semen samples from 12 infertile patients (9 with complete globozoospermia and 3 with partial globozoospermia) and 10 normozoospermic men (control) were examined by transmission electron microscopy and immunocytochemistry with antibodies against lamin B1. The presence of lamin A and progerin was assessed by reverse transcription-PCR. Whole exome sequencing was performed in three patients. In semen samples with complete and partial globozoospermia, lamin B1 was observed at the periphery of sperm nuclei, whereas lamin A and progerin were absent. Nuclear envelope pores were found in spermatozoa from both patient groups, regardless of morphology and chromatin condensation, in contrast to the control group. Non-condensed chromatin was present in 51%–81% of cases of complete globozoospermia and in 36%–79% of cases of partial globozoospermia. Homozygous DPY19L2 and SPATA16 variants were identified in two patients with partial globozoospermia and one patient with complete globozoospermia. An atypical nuclear membrane with abnormal nuclear pore distribution and lamin B1 localization was observed in spermatozoa from patients with both complete and partial globozoospermia. The genetic defects in the DPY19L2 and SPATA16 genes detected in patients from both globozoospermic groups suggest a generalized disruption of nuclear structure in globozoospermia, highlighting the genetic and phenotypic similarities between complete and partial globozoospermia.

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Section: Discussionmentioning

confidence: 99%

Atypical structure of the nuclear membrane, distribution of nuclear pores and lamin B1 in spermatozoa of patients with complete and partial globozoospermia

Bragina,

Kurchashova,

Suhomlinova

et al. 2024

Front. Genet.

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“…ICSI using round spermatids is not a suitable option, with a means to improve the outcome consisting in in vitro cultures of immature germ cells until more advanced stages. Consequently, the low pregnancy rates reflects the need for much more safer approaches [ 206 , 207 , 208 ] and we can witness a decline in the percentages of pregnancy rates via ART. The number of spermatids, testicular abnormalities, and apoptosis of germ cells post-vasectomy may worsen with extended periods of obstruction [ 209 ] and is viewed in the pregnancy rates.…”

Section: Discussionmentioning

confidence: 99%

A Narrative Review Discussing Vasectomy-Related Impact upon the Status of Oxidative Stress and Inflammation Biomarkers and Semen Microbiota

Doroftei

Ilie

Maftei

et al. 2023

JCM

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Background: Male contraceptive approaches besides tubal sterilization involve vasectomy and represent the method of choice among midlife men in developing countries thanks to many advantages. However, the subsidiary consequences of this intervention are insufficiently explored since the involved mechanisms may offer insight into a much more complex picture. Methods: Thus, in this manuscript, we aimed to reunite all available data by searching three separate academic database(s) (PubMed, Web of Knowledge, and Scopus) published in the past two decades by covering the interval 2000–2023 and using a predefined set of keywords and strings involving “oxidative stress” (OS), “inflammation”, and “semen microbiota” in combination with “humans”, “rats”, and “mice”. Results: By following all evidence that fits in the pre-, post-, and vasectomy reversal (VR) stages, we identified a total of n = 210 studies from which only n = 21 were finally included following two procedures of eligibility evaluation. Conclusions: The topic surrounding this intricate landscape has created debate since the current evidence is contradictory, limited, or does not exist. Starting from this consideration, we argue that further research is mandatory to decipher how a vasectomy might disturb homeostasis.

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“…This observation could suggest that Ca oscillations in pigs might induce oocyte activation largely through the mechanism triggered by the Zn sparks. Although the use of Zn chelator has not yet been approved in humans, other assisted artificial activation protocols have been approved [ 36 , 37 ]. Thus, outlining better activation procedures across different mammalian models is essential before application to humans.…”

Section: Discussionmentioning

confidence: 99%

Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation

Cabeza

Cámera

Briski

et al. 2022

Animals

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After sperm-oocyte fusion, intracytoplasmic rises of calcium (Ca) induce the release of zinc (Zn) out of the oocyte (Zn sparks). Both phenomena are known to play an essential role in the oocyte activation process. Our work aimed to explore different protocols for activating bovine and porcine oocytes using the novel zinc chelator 1,10-phenanthroline (PHEN) and to compare developmental rates and quality to bovine IVF and parthenogenetic ionomycin-induced embryos in both species. Different incubation conditions for the zinc chelator were tested, including its combination with ionomycin. Embryo quality was assessed by immunofluorescence of SOX2, SOX17, OCT4, and CDX2 and total cell number at the blastocyst stage. Even though blastocyst development was achieved using a zinc chelator in bovine, bypassing calcium oscillations, developmental rates, and blastocyst quality were compromised compared to embryos generated with sperm-induced or ionomycin calcium rise. On the contrary, zinc chelation is sufficient to trigger oocyte activation in porcine. Additionally, we determined the optimal exposure to PHEN for this species. Zinc chelation and artificial induction of calcium rise combined did not improve developmental competence. Our results contribute to understanding the role of zinc during oocyte activation and preimplantation embryo development across different mammalian species.

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Oocyte activation during round spermatid injection: state of the art

Cited by 6 publications

References 77 publications

Atypical structure of the nuclear membrane, distribution of nuclear pores and lamin B1 in spermatozoa of patients with complete and partial globozoospermia

Atypical structure of the nuclear membrane, distribution of nuclear pores and lamin B1 in spermatozoa of patients with complete and partial globozoospermia

A Narrative Review Discussing Vasectomy-Related Impact upon the Status of Oxidative Stress and Inflammation Biomarkers and Semen Microbiota

Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation

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