Oocyte donation: not all oocyte cryobanks are the same

Gianaroli, Luca; Ferraretti, Anna Pia; Perruzza, Davide; Terzuoli, Gaia; Azzena, Silvia; Crippa, Andor; Dworakowska, Aneta; Tabanelli, Carla; Magli, M. Cristina

doi:10.1016/j.rbmo.2021.10.015

Cited by 7 publications

(5 citation statements)

References 28 publications

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“…Our cumulative miscarriage rate of 24.7% is in line with the studies by Rienzi et al, 2020 [10] and Coccia et al, 2020 [25], where it was 21.5% and 23.8% respectively, and it is higher in comparison with the 11.4% of Gianaroli et al, 2022 [27]. The reason for this difference is not known, since the endometrial preparation was similar among the different Italian experiences.…”

Section: Discussionsupporting

confidence: 90%

“…On the other hand, by making a comparison with similar Italian experiences with imported cryopreserved oocytes [10,[25][26][27][28] or with imported frozen embryos [5], there is a consistency with our results.…”

Section: Discussionsupporting

confidence: 88%

“…The reason for this difference is not known, since the endometrial preparation was similar among the different Italian experiences. The only difference is that in the paper by Gianaroli et al [27], the progesterone for the luteal phase supplementation in the recipients was administered subcutaneously (50 mg/die). A recent systematic review on the endometrial preparation in egg donation or frozen embryo replacement cycles concluded that there is paucity of data on the impact of different routes (vaginal, im, sc, oral or rectal routes) of progesterone administration on reproductive outcomes in Hormone Replacement Treatment (HRT) cycles [29].…”

Section: Discussionmentioning

confidence: 99%

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The age of recipients and the number of received and inseminated oocytes affect the ART outcomes in a transnational oocyte donation program with vitrified oocytes: a single-center, seven-years’ experience

Marino

Volpes

Sammartano

et al. 2023

Preprint

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In Italy, egg donation is being allowed again following the ruling by the Constitutional Court n. 162/2014. Nevertheless, the number of voluntary donors is insignificant, probably because no reimbursement is provided. Therefore, the great majority of egg donation cycles are performed by using imported cryopreserved oocytes from foreign countries. However, recent evidence has questioned the overall efficacy of this strategy in comparison with the shipment of frozen sperm and vitrified embryos. A retrospective cohort study was conducted ANDROS Day Surgery Clinic Palermo, Italy in the period 2016–2022, using cryopreserved donated oocytes acquired from a single Spanish clinic (Next Fertility Valencia), with the objective of evaluating the efficiency of each batch of oocytes. The primary outcome was the cumulative ongoing pregnancy rate (COPR). The secondary outcomes were the cumulative clinical pregnancy rate (CCPR) and the miscarriage rate. The COPR totalled 36.8%, the CCPR 49.2% and the miscarriage rate 24.7%. The age of recipients and the number of received and inseminated oocytes were significantly associated with the cumulative ongoing and clinical pregnancies. Thus, the greater the number of oocytes, the higher the pregnancy rate, and the younger the recipients, the higher the success rates. In conclusion, our “transnational” donation program with vitrified oocytes has good success rates. The number of received and inseminated oocytes represents a crucial factor for increasing the cumulative pregnancy rates. Further interesting data regards the negative effect of advancing recipient age on success rates, outlining a possible, important role of age-related uterine factor on implantation mechanisms.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

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The age of recipients and the number of received and inseminated oocytes affect the ART outcomes in a transnational oocyte donation program with vitrified oocytes: a single-center, seven-years’ experience

Marino

Volpes

Sammartano

et al. 2023

Preprint

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“…Thus, variations in the efficiency of the vitrification/warming technique performed may lead to lower reproductive outcomes. The event of degeneration after warming is known to be centre- and operator-dependent [ 21 ]. Qualified as a highly skilled procedure requiring precision and speed, vitrification thus needs high-trained operators.…”

Section: Introductionmentioning

confidence: 99%

Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses

et al. 2022

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Background Indications of oocyte vitrification increased substantially over the last decades for clinical and ethical reasons. A semi-automated vitrification system was recently developed making each act of vitrification reproducible. In this study, we evaluated the efficiency of the semi-automated technique of oocyte vitrification by survival rate, morphometric assessment and resistance to empty micro-injection gesture as compared with a manual method. Additionally, we intended to evaluate transcriptomic consequences of both techniques using single-cell RNA-seq technology. Results Post-warming survival rate, oocyte surfaces and resistance to empty micro-injection were comparable between semi-automated and manual vitrification groups. Both oocyte vitrification techniques showed limited differences in the resulting transcriptomic profile of sibling oocytes since only 5 differentially expressed genes were identified. Additionally, there was no difference in median transcript integrity number or percentage of mitochondrial DNA between the two groups. However, a total of 108 genes were differentially expressed between fresh and vitrified oocytes (FDR < 0.05) and showed over-represented of genes related to important cellular process. Conclusions Our results provide reassurance about the influence of semi-automation as compared with the manual vitrification method. Concerning oocyte vitrification itself, no tight common transcriptomic signature associated has been observed across studies. Trial registration NCT03570073.

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“…Thus, variations in the e ciency of the vitri cation/warming technique performed may lead to lower reproductive outcomes. The event of degeneration after warming is known to be centre-and operator-dependent (Gianaroli et al, 2022). Quali ed as a highly skilled procedure requiring precision and speed, vitri cation thus needs high-trained operators.…”

Section: Introductionmentioning

confidence: 99%

Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses

Barberet,

Ducreux,

Bruno

et al. 2022

Preprint

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Background : Indications of oocyte vitrification increased substantially over the last decades for clinical and ethical reasons. A semi-automated vitrification system was recently developed making each act of vitrification reproducible. In this study, we evaluated the efficiency of the semi-automated technique of oocyte vitrification by survival rate, morphometric assessment and resistance to empty micro-injection gesture as compared with a manual method. Additionally, we intended to evaluate transcriptomic consequences of both techniques using single-cell RNA-seq technology.Results : Post-warming survival rate, oocyte surfaces and resistance to empty micro-injection were comparable between semi-automated and manual vitrification groups. Both oocyte vitrification techniques showed limited differences in the resulting transcriptomic profile of sibling oocytes since only 5 differentially expressed genes were identified. Additionally, there was no difference in median transcript integrity number or percentage of mitochondrial DNA between the two groups. However, a total of 108 genes were differentially expressed between fresh and vitrified oocytes (FDR<0.05) and showed over-represented of genes related to important cellular process.Conclusions : Our results provide reassurance about the influence of semi-automation as compared with the manual vitrification method. Concerning oocyte vitrification itself, no tight common transcriptomic signature associated has been observed across studies.

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Oocyte donation: not all oocyte cryobanks are the same

Cited by 7 publications

References 28 publications

The age of recipients and the number of received and inseminated oocytes affect the ART outcomes in a transnational oocyte donation program with vitrified oocytes: a single-center, seven-years’ experience

The age of recipients and the number of received and inseminated oocytes affect the ART outcomes in a transnational oocyte donation program with vitrified oocytes: a single-center, seven-years’ experience

Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses

Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses

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