Oogenesis in the mouse

Borum, Kirstine

doi:10.1016/0014-4827(61)90449-9

Cited by 308 publications

(69 citation statements)

References 12 publications

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“…Under these circumstances, inactivation of the X chromosome in meiotic prophase may be impaired in hybrid spermatocytes (34). By comparison, meiotic prophase in oogenesis has two active X chromosomes and meiosis is arrested at the dictyate stage just prior to MI (35). Degenerated spermatocytes were observed at late MI in the sterile hybrid males with dissociated X-Y pairing, which supports this hypothesis.…”

Section: Discussionsupporting

confidence: 73%

Genetic basis of X-Y chromosome dissociation and male sterility in interspecific hybrids.

Matsuda

Hirobe

Chapman

1991

Proc. Natl. Acad. Sci. U.S.A.

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A high frequency of X-Y chromosome dissociation (95%) was found at first meiotic metaphase (MI) in spermatocytes of interspecific hybrids between laboratory mice, C57BL/6J (BL/6) and Mus spretus, compared with an X-Y dissociation frequency ofonly 3-4% in parental mice. The X-Y dissociation in F, hybrids occurred before diakinesis rather than as a precocious dissociation at MI. The high X-Y dissociation was accompanied by spermatogenic breakdown after MI, resulting in male sterility. AU F, males were sterile and approximately half of the backcross males from fertile F1 females crossed with either BL/6 or M. spretus males were sterile. Male-sterility was highly correlated with X-Y dissociation in both backcrosses. AU of the mice with high X-Y dissociation were sterile and all of the males with low X-Y dissociation were fertile or subfertile. This correlation suggested that genetic divergence of the X-Y pairing region could contribute to the male sterile phenotype such that the BL/6 X chromosome would not pair with the M. spretus Y chromosome. The segregation of species-type alleles of amelogenin (Ameib andAmels), a distal X chromosome locus adjacent to the X-Y pairing region, was followed in backcross males that were analyzed for X-Y dissociation and sterility (we have used Amel as the designation for the mouse amelogenin locus; the current designation for this locus isAmg). A 95% concordance between Amelb with fertility and Amels with sterility was observed in backcrosses with BL/6, whereas the converse was observed in the backcross to M. spretus. These results imply that X-Y paring plays an important role in male fertility and suggest that genetic divergence in X-Y pairing region between Mus species can contribute to the reproductive barriers between species and the process of speciation.The X and Y chromosomes are normally associated end-toend during the first meiotic metaphase (MI) of spermatocytes in the male house mouse (1-4). However, a variable frequency of X and Y chromosome dissociation (0-50%) at MI of spermatocytes has been reported in various laboratory mouse strains and Mus musculus subspecies stocks (5-8). In addition to the variable within-strain dissociation, a high frequency of X-Y dissociation (50-90%) has been observed at MI of spermatocytes in interstrain hybrids or intersubspecies hybrids between laboratory mice and several Asian M. musculus subspecies that were genetically divergent from laboratory mice (7-9). In these cases, the observed X-Y dissociation involved a precocious dissociation of chromosomes that were once paired at pachytene, and there was no plausible correlation between the precocious X-Y dissociation with nondisjunction or fertility (8,9). A genetic analysis ofthe high X-Y dissociation in F1 hybrids between laboratory mice and Japanese wild mice, Mus molossinus, suggested that at least one heritable factor controlling the end-to-end association of the sex chromosome at MI was located on the common region of X and Y chromosomes (10). The genetic factor had no influenc...

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Section: Discussionsupporting

confidence: 73%

Genetic basis of X-Y chromosome dissociation and male sterility in interspecific hybrids.

Matsuda

Hirobe

Chapman

1991

Proc. Natl. Acad. Sci. U.S.A.

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“…Cell populations enriched for leptotene/zygotene oocytes, pachytene oocytes, and diplotene oocytes were obtained from fetal ovaries at 15.5 days p.c, 18.5 days p.c, and 21.5-days p.c, respectively (Borum 1961;McLaren 1988), whereas type-Ti prospermatogonia were obtained from fetal testes at 15.5 days p.c. and 18.5 days p.c.…”

Section: Piepaiation Of Dnamentioning

confidence: 99%

Developmental pattern of gene-specific DNA methylation in the mouse embryo and germ line.

Kafri¹,

Ariel²,

Brandeis³

et al. 1992

Genes Dev.

692

428

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Methylation patterns of specific genes have been studied by polymerase chain reaction and found to undergo dynamic changes in the germ line and early embryo. Some CpG sites are methylated in sperm DNA and unmodified in mature oocytes, indicating that the parental genomes have differential methylation profiles. These differences, however, are erased by a series of early embryonic demethylation and postblastula remodification events, which serve to reestablish the basic adult methylation pattern prior to organogenesis. During gametogenesis, all of these sites are unmethylated in primordial germ cells but eventually become remodified by 18.5 days postcoitum in both males and females. The final methylation profile of the mature germ cells is then formed by a multistep process of site-specific demethylation events. These results form a basis for the understanding of the biochemical mechanisms and role of DNA methylation in embryonic development.

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“…It has been considered that female mammals are born with a finite and non-renewing germ cell reserve (Borum, 1961;Peters, 1970;McLaren, 1984;Anderson and Hirshfield, 1992). However, several studies have challenged this doctrine in recent years (Johnson et al, 2004;Zou et al, 2009Zou et al, , 2011Pacchiarotti et al, 2010;Zhang et al, 2011;Hu et al, 2012;White et al, 2012;Zhou et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Production of offspring from a germline stem cell line derived from prepubertal ovaries of germline reporter mice

Zhang

2016

Mol. Hum. Reprod.

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study hypothesis: We investigated whether DEAD-box polypeptide 4 (DDX4) positive cells from post-natal ovaries of germline lineage reporter mice can be isolated based on endogenously expressed fluorescent proteins and used to establish a cell line for producing offspring. what is known already: In recent years, female germline stem cells (FGSCs) have been isolated from the ovaries of post-natal mice by magnetic-activated cell sorting or fluorescence-activated cell sorting (FACS) relying on an antibody against DDX4. However, whether DDX4-positive cells from post-natal ovaries of germline lineage reporter mice can be established without using an antibody, as well as a cell line established for producing offspring, remains unknown. study design, samples/materials, methods: To obtain the expected offspring (Ddx4-Cre;mT/mG mice), Ddx4-Cre mice were crossed with mT/mG mice. In the ovaries of Ddx4-Cre;mT/mG mice, germ cells were destined to express enhanced green fluorescent protein (EGFP) while somatic cells still express tandem dimer Tomato (tdTomato). Therefore, the germ cells could be clearly distinguished from somatic cells by fluorescent proteins. Then, we investigated the pattern of fluorescent cells in the ovaries of 21-day-old Ddx4-Cre;mT/mG mice under a fluorescent microscope. Germ cells were sorted by FACS without using antibody and used to establish a FGSC line. The FGSC line was analyzed by DDX4 immunostaining, Edu (5-ethynyl-2 ′ -deoxyuridine) labeling, and RT-PCR for germ cell markers. Finally, the physiological function of the FGSC line was examined by transplanting FGSCs into the ovaries of sterilized recipients and subsequent mating.main results and the role of chance: Firstly, we have successfully isolated FGSCs from the ovaries of 21-day-old Ddx4-Cre;mT/mG mice based on endogenously expressed fluorescent proteins. FACS was used to separate the cells and 2.3% of all viable cells was EGFP-positive germ cells. Subsequently, a FGSC line was established that was doubly positive for DDX4 immunostaining and Edu labeling. The mRNA expression of several germ cell markers in this cell line, such as Ddx4, Deleted in azoospermia-like (Dazl), B lymphocyte-induced maturation protein-1 (Blimp1), Stella and Fragilis, was detected. Lastly, the FGSC line was proven to be functional under physiological conditions, as offspring were produced after transplanting FGSCs into ovaries of sterilized recipients and a subsequent mating.limitations, reasons for caution: The molecular mechanisms of proliferation and differentiation of FGSCs in vivo and in vitro still need to be elucidated.wider implications of the findings: Our results confirm that DDX4-positive cells can be separated from post-natal mouse ovaries and used to establish cell lines that are functional in producing offspring, and provide further evidence for the existence of post-natal FGSCs in mammals. The Ddx4-Cre;mT/mG mouse strain is an ideal model for the isolation, characterization and propagation of FGSCs and is a useful tool for fully elucidating the molecu...

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Oogenesis in the mouse

Cited by 308 publications

References 12 publications

Genetic basis of X-Y chromosome dissociation and male sterility in interspecific hybrids.

Genetic basis of X-Y chromosome dissociation and male sterility in interspecific hybrids.

Developmental pattern of gene-specific DNA methylation in the mouse embryo and germ line.

Production of offspring from a germline stem cell line derived from prepubertal ovaries of germline reporter mice

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