Open a new window in the world of circulating microRNAs by merging ChemiRNA Tech with a Luminex platform

Abstract

miRNA analysis simplified using multi-ChemiRNA Tech.

SARS-CoV-2 viral RNA detection using the novel CoVradar device associated with the CoVreader smartphone app

“…1 D–E). DGL-Probes hybridize with complementary RNA fragments, the duplex are formed and DCL reaction takes place between the secondary amine of the DGL-probe and the aldehyde group of SMART-Base which is covalently linked to the DGL-Probe ( Marin-Romero et al, 2020 ; Marín-Romero et al, 2021 : Marín-Romero et al, 2022 ). Remarkably, CoVradar demonstrates high specificity.…”

MAGPIX and FLEXMAP 3D Luminex platforms for direct detection of miR-122-5p through dynamic chemical labelling

“…Functionalisation was carried out as described elsewhere. 25 Aldehyde-modified biotinylated cytosine nucleobase, called SMART-C Biotin (Table S1 and Fig. S1 †) as well as wash and Stabiltech buffer were provided by DESTINA Genomica S.L.…”

“…23 During the last years, our group demonstrated the advantages of detecting miR-122-5p by merging the Luminex xMAP technology with the Dynamic Chemical Labelling (DCL) technology. [24][25][26][27] Luminex xMAP technology is known to be a powerful and versatile platform for the multiplexed detection of various biomarkers within biological fluids. [28][29][30] The core of the Luminex technology lies in utilising Luminex microspheres, which are micro-beads made of a paramagnetic material.…”

“…To address these needs, our team has devoted substantial effort to integrate our unique DCL technology with the Luminex MAGPIX system over the years. [24][25][26][27] DCL technology enables the direct detection of miRs, eliminating the requirement for pre-extraction and pre-amplification while maintaining specificity and sensitivity. 37,38 DCL leverages a merge of biotinylated aldehyde-modified nucleobases, referred to as SMART-C Biotin and modified peptide nucleic acid (PNA) capture probes synthesised with an abasic position, known as DGL-Probes.…”

Nanomaterials for miRNA detection: the hybridization chain reaction strategy

“…[1][2][3] Also, it is difficult to monitor health checkups in many countries due to their high population and lack of resources. However, it can be achieved by developing simple techniques to detect microribonucleic acids (miRNAs) in various cell samples [4][5][6][7][8][9] These are noncoding short-sequence RNAs that are helpful in the expression of a gene. Upon hybridization with messenger RNA, miRNAs cause a negative regulation of gene transcription.…”