Open Probe fast GC–MS—Real time analysis with separation

Amirav, Aviv; Keshet, Uri; Alon, Tal; Fialkov, Alexander B.

doi:10.1016/j.ijms.2014.08.002

Cited by 12 publications

(23 citation statements)

References 18 publications

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“…The Agilent Chemstation measured signal‐to‐noise ratio is 13 000 in peak to peak and several thousand near the TNT peak. When this analysis is used, the human fingerprint wax compounds are reduced compared with the target TNT as can be observed in comparison with another Open Probe analysis of TNT on a human hand . The TNT separation time by the Open Probe fast GC was 40 s, and the full analysis cycle time was 55 s.…”

Section: Resultsmentioning

confidence: 93%

“…An auxiliary helium gas line with its miniaturized pressure regulator and frit flow control element (not shown) serves to provide the needed helium flow rate. Unlike the system that is described in our previous publication, the combination of Open Probe fast GC with MS with standard EI requires the reduction of the injection time in view of the relatively low column flow rate (typically 1–2 ml/min) that is used. Consequently, a small micropump was added, followed by a frit flow restrictor element and a solenoid valve that served to pump the split gas line.…”

Section: Experimental—open Probe Fast Gc–ms System Descriptionmentioning

confidence: 99%

“…Later on, we developed an Open Probe device that enables real‐time analysis via ambient pressure sample desorption followed by in‐vacuum EI and MS analysis with the low‐cost MS of GC–MS . We further developed a low thermal mass (LTM) ultra‐fast GC that enables <1‐min full analysis cycle time with typical separation times of 30 s and combined the Open Probe and fast GC into an Open Probe fast GC–MS that provided real‐time analysis with separation and library‐based sample identification including structures and names down to the isomer level …”

Section: Introductionmentioning

confidence: 99%

“…The Open Probe fast GC–MS as described in Ref. was focused on its combination with GC–MS with Cold EI . Consequently, the goal of this article is to describe the combination of the Open Probe fast GC with GC–MS with standard EI and further demonstrate its usefulness and capabilities with new applications and performance demonstrations.…”

Section: Introductionmentioning

confidence: 99%

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Open Probe fast GC–MS — combining ambient sampling ultra‐fast separation and in‐vacuum ionization for real‐time analysis

et al. 2017

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An Open Probe inlet was combined with a low thermal mass ultra-fast gas chromatograph (GC), in-vacuum electron ionization ion source and a mass spectrometer (MS) of GC-MS for obtaining real-time analysis with separation. The Open Probe enables ambient sampling via sample vaporization in an oven that is open to room air, and the ultra-fast GC provides ~30-s separation, while if no separation is required, it can act as a transfer line with 2 to 3-s sample transfer time. Sample analysis is as simple as touching the sample, pushing the sample holder into the Open Probe oven and obtaining the results in 30 s. The Open Probe fast GC was mounted on a standard Agilent 7890 GC that was coupled with an Agilent 5977A MS. Open Probe fast GC-MS provides real-time analysis combined with GC separation and library identification, and it uses the low-cost MS of GC-MS. The operation of Open Probe fast GC-MS is demonstrated in the 30-s separation and 50-s full analysis cycle time of tetrahydrocannabinol and cannabinol in Cannabis flower, sub 1-min analysis of trace trinitrotoluene transferred from a finger onto a glass surface, vitamin E in canola oil, sterols in olive oil, polybrominated flame retardants in plastics, alprazolam in Xanax drug pill and free fatty acids and cholesterol in human blood. The extrapolated limit of detection for pyrene is <1 fg, but the concentration is too high and the software noise calculation is untrustworthy. The broad range of compounds amenable for analysis is demonstrated in the analysis of reserpine. The possible use with alternate standard GC-MS and Open Probe fast GC-MS is demonstrated in the analysis of heroin in its street drug powder. The use of Open Probe with the fast GC acting as a transfer line is demonstrated in <10-s analysis without separation of ibuprofen and estradiol. Copyright © 2017 John Wiley & Sons, Ltd.

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Section: Resultsmentioning

confidence: 93%

Section: Experimental—open Probe Fast Gc–ms System Descriptionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Open Probe fast GC–MS — combining ambient sampling ultra‐fast separation and in‐vacuum ionization for real‐time analysis

et al. 2017

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“…Examples are flow-injection (FI), direct analysis in real time (DART), desorption electrospray ionization (DESI), laser-ablation electrospray ionization (LAESI), and atmospheric solids analysis probe (ASAP) and open probe (OP) fast gas chromatography. Examples of applications are recreational drug screening using FI (Alechaga et al, 2015), small molecule quantitation in plasma without sample preparation and chromatographic separation using DART (Zhao et al, 2008), lipid characterization in biological samples using DESI (Eberlin et al, 2011), metabolite screening in bodily fluids using LAESI (Nemes and Vertes, 2007), polycyclic aromatic hydrocarbons in human saliva and urine using ASAP (Carrizo et al, 2015), and illegal drugs using OP fast gas chromatography (Amirav et al, 2014). …”

Section: Advances In Analytical Tools For Profiling the Environmenmentioning

confidence: 99%

Trends in the application of high-resolution mass spectrometry for human biomonitoring: An analytical primer to studying the environmental chemical space of the human exposome

Andra

Austin

Patel

et al. 2017

Environment International

130

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Global profiling of xenobiotics in human matrices in an untargeted mode is gaining attention for studying the environmental chemical space of the human exposome. Defined as the study of a comprehensive inclusion of environmental influences and associated biological responses, human exposome science is currently evolving out of the metabolomics science. In analogy to the latter, the development and applications of high resolution mass spectrometry (HRMS) has shown potential and promise to greatly expand our ability to capture the broad spectrum of environmental chemicals in exposome studies. HRMS can perform both untargeted and targeted analysis because of its capability of full- and/or tandem-mass spectrum acquisition at high mass accuracy with good sensitivity. The collected data from target, suspect and non-target screening can be used not only for the identification of environmental chemical contaminants in human matrices prospectively but also retrospectively. This review covers recent trends and advances in this field. We focus on advances and applications of HRMS in human biomonitoring studies, and data acquisition and mining. The acquired insights provide stepping stones to improve understanding of the human exposome by applying HRMS, and the challenges and prospects for future research.

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Whole blood analysis for medical diagnostics by GC‐MS with Cold EI

et al. 2022

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This study covers a new method and related instrumentation for whole blood analysis for medical diagnostics. Two‐μL whole blood samples were collected using “minimal invasive” diabetes lancet and placed on a thin glass rod mounted on a newly designed BloodProbe. The BloodProbe with the whole blood sample was inserted directly into a ChromatoProbe mounted on the GC inlet, and thus, no sample preparation was involved. The analysis was performed within 10 min using a GC‐MS with Cold EI that is based on interfacing GC and MS with supersonic molecular beams (SMB) along with electron ionization of vibrationally cold sample compounds in the SMB (hence the name Cold EI). Our blood analysis revealed several observations: (1) Detailed mass chromatograms were generated with full range of all the nonpolar lipids in blood including fatty acids, cholesterol, cholesteryl esters, vitamin E, monoglycerides, diglycerides, and triglycerides. (2) The analysis of whole blood was found to be as informative as the conventional clinical analysis of blood serum. (3) Cholesteryl esters were more sensitive than free cholesterol alone to the effect of diet of obese people. (4) Major enhancement of several fatty acid methyl esters was found in the blood of a cancer patient with liver dysfunction. (5) Vitamin E as both α‐ and β‐tocopherol was found with person‐dependent ratio of these two compounds. (6) Elemental sulfur S8 was identified in blood. (7) Several drugs and other compounds were found and need further study of their correlation to medical issues.

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Open Probe fast GC–MS—Real time analysis with separation

Cited by 12 publications

References 18 publications

Open Probe fast GC–MS — combining ambient sampling ultra‐fast separation and in‐vacuum ionization for real‐time analysis

Open Probe fast GC–MS — combining ambient sampling ultra‐fast separation and in‐vacuum ionization for real‐time analysis

Trends in the application of high-resolution mass spectrometry for human biomonitoring: An analytical primer to studying the environmental chemical space of the human exposome

Whole blood analysis for medical diagnostics by GC‐MS with Cold EI

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