Opera Production I: A Handbook

Goldovsky, Boris; Eaton, Quaintance

doi:10.2307/896317

Cited by 1 publication

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“…The gene coexpression network of the 2911 differentially expressed genes (Supplemental Data Set 1) of the Mt-abi5 mutants was constructed using the normalized intensities of the wild-type and Mt-abi5 mutants together with those obtained at 15 time points during seed maturation, derived from Righetti et al (2015). The network was constructed using Biolayout software (Goldovsky et al, 2005) with a correction value of 0.92, giving rise to a network with 1147 nodes and 2409 edges. Gene interactions were visualized using the open source software Cytoscape (version 3.3.0) with an edge-weighted spring-embedded layout.…”

Section: Rna Extraction Microarray Analysis and Gene Coexpression Nmentioning

confidence: 99%

ABI5 Is a Regulator of Seed Maturation and Longevity in Legumes

et al. 2016

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The preservation of our genetic resources and production of high-quality seeds depends on their ability to remain viable and vigorous during storage. In a quantitative trait locus analysis on seed longevity in Medicago truncatula, we identified the bZIP transcription factor ABSCISIC ACID INSENSITIVE5 (ABI5). Characterization of Mt-abi5 insertion mutant seeds revealed that both the acquisition of longevity and dormancy were severely impaired. Using transcriptomes of developing Mt-abi5 seeds, we created a gene coexpression network and revealed ABI5 as a regulator of gene modules with functions related to raffinose family oligosaccharide (RFO) metabolism, late embryogenesis abundant (LEA) proteins, and photosynthesis-associated nuclear genes (PhANGs). Lower RFO contents in Mt-abi5 seeds were linked to the regulation of SEED IMBIBITION PROTEIN1 Proteomic analysis confirmed that a set of LEA polypeptides was reduced in mature Mt-abi5 seeds, whereas the absence of repression of PhANG in mature Mt-abi5 seeds was accompanied by chlorophyll and carotenoid retention. This resulted in a stress response in Mt-abi5 seeds, evident from an increase in α-tocopherol and upregulation of genes related to programmed cell death and protein folding. Characterization of abi5 mutants in a second legume species, pea (Pisum sativum), confirmed a role for ABI5 in the regulation of longevity, seed degreening, and RFO accumulation, identifying ABI5 as a prominent regulator of late seed maturation in legumes.

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Section: Rna Extraction Microarray Analysis and Gene Coexpression Nmentioning

confidence: 99%