Operant conditioning paradigm for juxtacellular recordings in functionally identified cortical neurons during motor execution in head-fixed rats

Santana-Chávez, Gabriela; Rodriguez-Moreno, Paola; López‐Hidalgo, Mónica; Olivares-Moreno, Rafael; Moreno-López, Yunuen; Rojas-Piloni, Gerardo

doi:10.1016/j.jneumeth.2019.108454

Cited by 2 publications

(2 citation statements)

References 24 publications

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“…This was to acclimate them to electrode manipulation and human-rat interactions. At P90 surgical procedure was performed; the technique was adapted from (Santana-Chávez et al 2020; Rigalli and Di Loreto 2016; Matsumiya et al 2012) (Figure 2).…”

Section: Methodsmentioning

confidence: 99%

Focal electrical stimulation on an alcohol disorder model using MRI-compatible chronic neural monopolar carbon fiber electrodes

López-Castro

Ángeles-Valdez

Rojas-Piloni

et al. 2022

Preprint

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Neuromodulation interventions, such as Deep Brain Stimulation (DBS) and repeated transcranial magnetic stimulation (rTMS), are proposed as possible new complementary therapies to treat substance use disorders (SUD) such as alcohol use disorder (AUD). It is hypothesized that neuromodulation may induce neural plasticity in the reward and frontostriatal systems via electrical field induction, possibly reducing symptoms. Preclinical self-administration rodent models of AUD may help us gain insight into the effects of neuromodulation therapies on different pathology, as well as the neural mechanisms behind the positive effects. DBS, or any type of brain stimulation using intracranial electrodes in rodents, would benefit from the use of MRI to study the longitudinal effects and mechanisms of stimulation as well as novel targets, as it is a non-invasive technique that allows the analysis of structural and functional changes in the brain. To do this, there is a need for MRI-compatible electrodes that allow for MRI acquisition with minimal distortion of the magnetic field. In this protocol, we present a method for the construction and surgery of chronically implantable monopolar carbon electrodes for use in rats. Unlike conventional electrodes, carbon electrodes are resistant to high temperatures, flexible, and generate fewer artifacts in MRI compared to conventional ones. We validated its use by using a focal electrical stimulation high-frequency (20 Hz) protocol that lasted ~10 sessions. We propose that this technique can also be used for the research of the neurophysiological bases of the neuromodulatory treatment in other preclinical substance use disorders (SUD) models.

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Section: Methodsmentioning

confidence: 99%

Focal electrical stimulation on an alcohol disorder model using MRI-compatible chronic neural monopolar carbon fiber electrodes

López-Castro

Ángeles-Valdez

Rojas-Piloni

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…This was to acclimate them to electrode manipulation and human-rat interactions. At P90 surgical procedure was performed; the technique was adapted from Matsumiya et al (2012) , Rigalli and Di Loreto (2016) and Santana-Chávez et al (2020) ( Figure 2 ).…”

Section: Materials and Equipmentmentioning

confidence: 99%

Focal electrical stimulation on an alcohol disorder model using magnetic resonance imaging-compatible chronic neural monopolar carbon fiber electrodes

López-Castro

Ángeles-Valdez²,

Rojas-Piloni³

et al. 2022

Front. Neurosci.

Self Cite

View full text Add to dashboard Cite

Neuromodulation interventions, such as Deep Brain Stimulation (DBS) and repeated transcranial magnetic stimulation (rTMS), are proposed as possible new complementary therapies to treat substance use disorders (SUD) such as alcohol use disorder (AUD). It is hypothesized that neuromodulation may induce neural plasticity in the reward and frontostriatal systems via electrical field induction, possibly reducing symptoms. Preclinical self-administration rodent models of AUD may help us gain insight into the effects of neuromodulation therapies on different pathology, as well as the neural mechanisms behind the positive effects. DBS, or any type of brain stimulation using intracranial electrodes in rodents, would benefit from the use of magnetic resonance imaging (MRI) to study the longitudinal effects and mechanisms of stimulation as well as novel targets, as it is a non-invasive technique that allows the analysis of structural and functional changes in the brain. To do this, there is a need for MRI-compatible electrodes that allow for MRI acquisition with minimal distortion of the magnetic field. In this protocol, we present a method for the construction and surgery of chronically implantable monopolar carbon electrodes for use in rats. Unlike conventional electrodes, carbon electrodes are resistant to high temperatures, flexible, and generate fewer artifacts in MRI compared to conventional ones. We validated its use by using a focal electrical stimulation high-frequency (20 Hz) protocol that lasted ∼10 sessions. We propose that this technique can also be used for the research of the neurophysiological bases of the neuromodulatory treatment in other preclinical substance use disorders (SUD) models.

show abstract

Operant conditioning paradigm for juxtacellular recordings in functionally identified cortical neurons during motor execution in head-fixed rats

Cited by 2 publications

References 24 publications

Focal electrical stimulation on an alcohol disorder model using MRI-compatible chronic neural monopolar carbon fiber electrodes

Focal electrical stimulation on an alcohol disorder model using MRI-compatible chronic neural monopolar carbon fiber electrodes

Focal electrical stimulation on an alcohol disorder model using magnetic resonance imaging-compatible chronic neural monopolar carbon fiber electrodes

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