OPG and sRANKL Serum Concentrations in Osteopenic, Postmenopausal Women After 2-Year Genistein Administration

Marini, Herbert; Minutoli, Letteria; Polito, Francesca; Bitto, Alessandra; Altavilla, Domenica; Atteritano, Marco; Gaudio, Agostino; Mazzaferro, S.; Frisina, Alessia; Frisina, N; Lubrano, Carla; Bonaiuto, Michele; D’Anna, Rosario; Cannata, Maria Letizia; Corrado, Francesco; Cancellieri, Francesco; Faraci, Marianna; Marini, Rolando; Adamo, Elena Bianca; Wilson, Steven; Squadrito, Francesco

doi:10.1359/jbmr.080201

Cited by 72 publications

(63 citation statements)

References 34 publications

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“…In the present study low dose genistein treatment increased the expression of ALP, OC, and OPG at the serum level and mRNA level both in vivo and in vitro. Low-dose genistein treatment also decreased the expression of RANKL and the RANKL/OPG ratio in agreement with previous studies [20,[41][42][43][44] . These data suggest that normal doses of genistein improve bone formation and inhibit bone resorption.…”

Section: Effect Of Er Silencing On the Effect Of Genistein Treatmentsupporting

confidence: 92%

Dose-dependent effects of genistein on bone homeostasis in rats' mandibular subchondral bone

Xing

Wang

et al. 2011

Acta Pharmacol Sin

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Aim: To investigate the effect of genistein on bone homeostasis in mandibular subchondral bone of rats. Methods: Female SD rats were administered with genistein (10 and 50 mg/kg) or placebo by oral gavage for 6 weeks. Then the animals were sacrificed, and histomorphology and micro-structure of mandibular condyle were examined using HE staining and micro-CT analysis, respectively. The expression levels of alkaline phosphatase (ALP), osteocalcin (OC), osteoprotegerin (OPG), the receptor activator of nuclear factor κB ligand (RANKL) and estrogen receptors (ERs) in mandibular condyle were detected using real-time PCR. Cultured osteoblasts were prepared from rat mandibular condyle for in in vitro study. The cells were treated with genistein (10 -7 or 10 -4 mol/L) for 48 h. The expression of the bone homeostasis-associated factors and estrogen receptors (ERs) was detected using realtime PCR, and ER silencing was performed. Results: At both the low-and high-doses, genistein significantly increased the bone mineral density (BMD) and bone volume, and resulted in thicker subchondral trabecular bone in vivo. In both in vivo and in vitro study, the low-dose genistein significantly increased the expression of ALP, OC and OPG, but decreased the expression of RANKL and the RANKL/OPG ratio. The high-dose genistein decreased the expression of all these bone homeostasis-associated factors. Both the low and high doses of genistein significantly increased the expression of ERβ, while ERα expression was increased by the low dose genistein and decreased by the high dose genistein. ERβ silencing abrogated most of the effects of genistein treatment. Conclusion: In rat mandibular condylar subchondral bone, low-dose genistein increases bone formation and inhibit bone resorption, while excess genistein inhibits both bone formation and resorption. The effects of genistein were predominantly mediated through ERβ.

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Section: Effect Of Er Silencing On the Effect Of Genistein Treatmentsupporting

confidence: 92%

Dose-dependent effects of genistein on bone homeostasis in rats' mandibular subchondral bone

Xing

Wang

et al. 2011

Acta Pharmacol Sin

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“…This effect was substantially equivalent to that observed for HRT in postmenopausal women [19]. Furthermore, we suggested that these positive effects could be related to the action of genistein on the soluble receptor activator of nuclear factor κB ligand/osteoprotegerin balance in terms of an improvement of bone turnover [21,22]. In both trials, isolated genistein administration exhibited a highly standardized bioavailability and pharmacokinetic behavior.…”

Section: Introductionsupporting

confidence: 60%

Genistein effects on quantitative ultrasound parameters and bone mineral density in osteopenic postmenopausal women

et al. 2009

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“…The effective dose (1,000 mg/kg) of SWH found in this study can be achieved (220-g crude herb) in women with a body weight of 50 kg based on the calculation of human-animal transformation. The dose (50 mg/kg) of genistein applied in this study could be derived as 200 mg/day for postmenopausal women with oral administration, and this derived clinical dose is higher than the regular dose (50-100 mg/day) of genistein utilized in previous clinical tests [23][24][25].…”

Section: Discussionmentioning

confidence: 99%

Study of the mechanisms by which Sambucus williamsii HANCE extract exert protective effects against ovariectomy-induced osteoporosis in vivo

Zhang

Wan

et al. 2010

Osteoporos Int

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OPG and sRANKL Serum Concentrations in Osteopenic, Postmenopausal Women After 2-Year Genistein Administration

Cited by 72 publications

References 34 publications

Dose-dependent effects of genistein on bone homeostasis in rats' mandibular subchondral bone

Dose-dependent effects of genistein on bone homeostasis in rats' mandibular subchondral bone

Genistein effects on quantitative ultrasound parameters and bone mineral density in osteopenic postmenopausal women

Study of the mechanisms by which Sambucus williamsii HANCE extract exert protective effects against ovariectomy-induced osteoporosis in vivo

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