Opioids as an alternative to amide-type local anaesthetics for intra-articular application

Ickert, Irina; Herten, Monika; Vogl, Melanie; Ziskoven, Christoph; Zilkens, Christoph; Krauspe, Rüdiger; Kircher, Jörn

doi:10.1007/s00167-014-2989-2

Cited by 26 publications

(45 citation statements)

References 40 publications

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“…Ickert et al exposed human chondrocytes to 0.75% ropivacaine and 0.5% bupivacaine, morphine, and controls for 15, 60, and 240 minutes . They found time‐dependent cytotoxic effects with a significant decline in cell viability at all three time points for bupivicaine .…”

Section: Resultsmentioning

confidence: 99%

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Chondrotoxic Effects of Local Anesthetics on Human Knee Articular Cartilage: A Systematic Review

Jayaram¹,

Kennedy

Yeh

et al. 2019

PM&R

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Objective To review the current literature on the effects of intraarticular local anesthetics on human knee articular cartilage. Literature Survey PubMed; MEDLINE; SCOPUS; PEDro; CINAHL databases (1/1990‐06/1/2018) were searched for local anesthetic effects on human knee articular cartilage. Methodology Sixteen studies met the inclusion criteria, with outcome measures assessing chondrocyte viability, morphology, and histology. A systematic review was performed using PRISMA guidelines. Synthesis Seven studies were identified evaluating lidocaine, with five of them demonstrating statistically significant chondrotoxic effects. Fourteen studies examined bupivacaine, with all but one study demonstrating a chondrotoxic effect. Eight studies examined ropivacaine and found a dose‐dependent chondrotoxicity starting at 0.75%. Two studies evaluated levobupivacaine showing chondrotoxicity, with one study showing it to be more chondrotoxic than bupivacaine. One study looked at mepivacaine and showed it to have more chondrotoxicity than ropivacaine. When studied the chondrotoxicity was found to be both dose and time dependent. Also, the addition of corticosteroids appeared to worsen the chondrotoxic effects. Conclusions Lidocaine, bupivacaine, ropivacaine, levobupivacaine, and mepivacaine were reported to have dose‐ and time‐dependent deleterious effects on chondrocytes that appeared to be made worse by the coadministration of corticosteroids. Ropivacaine at concentrations of 0.5% or less was found to be the least chondrotoxic anesthetic. Level of Evidence I

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Section: Resultsmentioning

confidence: 99%

“…A cumulative score of 7 was the highest achievable score. Three of the 16 studies achieved a score of 3 or less, whereas all other studies had either 4 or 5 points (Table ).…”

Section: Methodsmentioning

confidence: 99%

Chondrotoxic Effects of Local Anesthetics on Human Knee Articular Cartilage: A Systematic Review

Jayaram¹,

Kennedy

Yeh

et al. 2019

PM&R

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“…The mitochondrial adenosine triphosphate (ATP) concentration was measured using the BacTiter-Glo™ Microbial Cell Viability assay (Promega, Mannheim, Germany) as described before (Ickert et al, 2015; Herten et al, 2017). The number of viable bacteria is quantified by their amount of ATP and is directly proportional to the ATP concentration.…”

Section: Methodsmentioning

confidence: 99%

Rapid in Vitro Quantification of S. aureus Biofilms on Vascular Graft Surfaces

et al. 2017

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Objectives: Increasing resistance of microorganisms and particularly tolerance of bacterial biofilms against antibiotics require the need for alternative antimicrobial substances. S. aureus is the most frequent pathogen causing vascular graft infections. In order to evaluate the antimicrobial efficacy, quantification of the bacterial biofilms is necessary. Aim of the present study was the validation of an in vitro model for quantification of bacterial biofilm on vascular graft surfaces using three different assays.Methods: Standardized discs of vascular graft material (Dacron or PTFE) or polystyrene (PS) as control surface with 0.25 cm2 surface area were inoculated with 10−3 diluted overnight culture of three biofilm-producing S. aureus isolates (BEB-029, BEB-295, SH1000) in 96-well PS culture plates. After incubation for 4 and 18 h, the biofilm was determined by three different methods: (a) mitochondrial ATP concentration as measure of bacterial viability (ATP), (b) crystal violet staining (Cry), and (c) vital cell count by calculation of colony-forming units (CFU). The experiments were performed three times. Quadruplicates were used for each isolate, time point, and method. In parallel, bacterial biofilms were documented via scanning electron microscopy.Results: All three methods could quantify biofilms on the PS control. Time needed was 0:40, 13:10, and 14:30 h for ATP, Cry, and CFU, respectively. The Cry assay could not be used for vascular graft surfaces due to high unspecific background staining. However, ATP assay and CFU count showed comparable results on vascular graft material and control. The correlations between ATP and CFU assay differed according to the surface and incubation time and were significant only after 4 h on Dacron (BEB-029, p = 0.013) and on PS (BEB-029, p < 0.001). Between ATP and Cry assay on PS, a significant correlation could be detected after 4 h (BEB-295, p = 0.027) and after 18 h (all three strains, p < 0.026). The reproducibility of the ATP-assay presented as inter-assay-variance of 2.1 and as intra-assay variance of 8.1 on polystyrene.Conclusion: The in-vitro model reproducibly quantifies biofilm on standardized vascular graft surfaces with ATP assay as detection system. The ATP assay allows accelerated microbial quantification, however the correlation with the CFU assay may be strain- and surface-dependent.

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“…Cell viability was analyzed by measuring the mitochondrial adenosine triphosphate (ATP) concentration using the CellTiter-Glo ® Luminescence assay (Promega, Mannheim, Germany), as described before [26]. The number of viable cells is quantified by their amount of ATP and is directly proportional to the ATP concentration.…”

Section: Methodsmentioning

confidence: 99%

Vascular Graft Impregnation with Antibiotics: The Influence of High Concentrations of Rifampin, Vancomycin, Daptomycin, and Bacteriophage Endolysin HY-133 on Viability of Vascular Cells

Herten

Idelevich²,

Sielker

et al. 2017

Med Sci Monit Basic Res

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BackgroundRifampin-soaked synthetic prosthetic grafts have been widely used for prevention or treatment of vascular graft infections (VGIs).This in vitro study investigated the effect of the antibiotics daptomycin and vancomycin and the new recombinant bacteriophage endolysin HY-133 on vascular cells, as potential alternatives compared to rifampin.Material/MethodsPrimary human ECs, vascular smooth muscle cells (vSMC), and fibroblasts were cultivated in 96-well plates and incubated with rifampin, daptomycin, vancomycin, and endolysin HY-133 for 24 h. Subsequently, after washing, cell viability was determined by measuring mitochondrial ATP concentration. Antibiotics were used in their corresponding minimum and maximum serum concentrations, in decimal multiples and in maximum soaking concentration. The experiments were performed in triplicate.ResultsThe 10-fold max serum concentrations of rifampin, daptomycin, and vancomycin did not influence viability of EC and vSMC (100 μg/ml, p>0.170). Higher concentrations of rifampin (>1 mg/ml) significantly (p<0.001) reduced cell viability of all cell types. For the other antibiotics, high concentrations (close to maximum soaking concentration) were most cytotoxic for EC and vSMC and fibroblasts (p<0.001). Endolysin did not display any cytotoxicity towards vascular cells.ConclusionsResults of this in vitro study show the high cytotoxicity of rifampin against vascular cells, and may re-initiate the discussion about the benefit of prophylactic pre-soaking in high concentrations of rifampin. Further studies are necessary to determine the influence of rifampin on the restoration of vessel functionality versus its prophylactic effect against VGIs. Future use of recombinant phage endolysins for alternative prophylactic strategies needs further investigations.

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Opioids as an alternative to amide-type local anaesthetics for intra-articular application

Cited by 26 publications

References 40 publications

Chondrotoxic Effects of Local Anesthetics on Human Knee Articular Cartilage: A Systematic Review

Chondrotoxic Effects of Local Anesthetics on Human Knee Articular Cartilage: A Systematic Review

Rapid in Vitro Quantification of S. aureus Biofilms on Vascular Graft Surfaces

Vascular Graft Impregnation with Antibiotics: The Influence of High Concentrations of Rifampin, Vancomycin, Daptomycin, and Bacteriophage Endolysin HY-133 on Viability of Vascular Cells

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