Opportunistic Microorganisms Identification Using a Modern Laboratory Technique

Abstract

Introduction: Recent studies show that 85% of cases of non-traumatic amputations in patients with diabetes mellitus are preceded by a wound infection of the foot, this confirms the need for rational antimicrobial therapy. Therefore, competent rapid identification of microorganisms is a priority task of modern microbiology. Matrix-Assisted Laser Desorption Ionization TOFstands for Time of Flight Mass Spectrometry (MALDI-TOF MS) is considered as the revolution in bacteriology, to identify pathogens isolated in a clinical microbiological laboratory. In this regard, MALDI-TOF MS is an ideal solution for the rapid identification of DFS Objectives: To Explore of Modern Laboratory Technique (MALDI-TOF MS) for the identification of opportunistic microorganisms in patients with Diabetic Foot Syndrome (DFS). Materials and Methods: Was performed from September 2012 to May 2015 on patients admitted to the Emergency Room of the Hospital General de México. 72 patients with (DFS) diabetic foot syndrome were investigated for the period of 2013-2015 years. The comparison group consisted of 30 patients with chronic purulent inflammatory diseases of the lower extremities not suffering from diabetes mellitus. Identification of microorganisms was carried out using MALDI-TOF spectrometry (BRUKER 2012). Results: This study revealed the prevalence gram-positive bacteria in patients with DFS. The dominant microorganism in the etiology of DFS was S. aureus, which occurred mostly as monoculture. Coagulase-Negative Staphylococci (CoNS) were represented mainly by S. haemolyticus and S. epidermidis generally recognized as a pathogen of medical devices in our case does not have an important role. Enterobacteria and non-fermenting bacteria represented Gram-negative bacteria.