Opportunities and challenges for the baculovirus expression system

Oers, Monique M. van

doi:10.1016/j.jip.2011.05.001

Cited by 132 publications

(95 citation statements)

References 164 publications

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“…Members of this family of occluded, double-stranded DNA viruses have proved remarkably valuable as pest control agents (12), as protein expression systems (13)(14)(15)(16), for vaccine production (17,18), and as potential therapeutic gene delivery vectors (19). Members of the genus Alphabaculovirus infect lepidopteran insects through host consumption of plant material contaminated by virus-containing occlusion bodies (OBs).…”

mentioning

confidence: 99%

Superinfection Exclusion in Alphabaculovirus Infections Is Concomitant with Actin Reorganization

Beperet

Irons

Simón

et al. 2014

J Virol

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Superinfection exclusion is the ability of an established virus to interfere with a second virus infection. This effect was studied in vitro during lepidopteran-specific nucleopolyhedrovirus (genus observed following inoculation with mixtures of these two phylogenetically distant nucleopolyhedroviruses-AcMNPV and SfMNPV-but at a frequency lower than predicted, suggesting interspecific virus interference during infection or replication. The temporal window of infection is likely necessary to maintain genotypic diversity that favors virus survival but also permits dual infection by heterospecific alphabaculoviruses.IMPORTANCE Infection of a cell by more than one virus particle implies sharing of cell resources. We show that multiple infection, by closely related or distantly related baculoviruses, is possible only during a brief window of time that allows additional virus particles to enter an infected cell over a period of ca. 16 h but then blocks multiple infections as newly generated virus particles begin to leave the infected cell. This temporal window has two important consequences. First, it allows multiple genotypes to almost simultaneously infect cells within the host, thus generating genetically diverse virus particles for transmission. Second, it provides a mechanism by which different viruses replicating in the same cell nucleus can exchange genetic material, so that the progeny viruses may be a mosaic of genes from each of the parental viruses. This opens a completely new avenue of research into the evolution of these insect pathogens.

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mentioning

confidence: 99%

Superinfection Exclusion in Alphabaculovirus Infections Is Concomitant with Actin Reorganization

Beperet

Irons

Simón

et al. 2014

J Virol

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“…This has been accomplished in part by use of baculovirus expression vector systems (BEVs) for heterologous recombinant protein production and gene transfer. The principle behind the use of BEVs is based on use of polyhedrin and p10 promoters to drive the expression of foreign genes in cell culture or in vivo (van Oers, 2011;Summers, 2006). BEVs continue to evolve to new and robust systems.…”

Section: Baculoviruses and Biomedical Applicationsmentioning

confidence: 99%

“…BEVs continue to evolve to new and robust systems. For instance, the latest vectors (flashBACultra/BacMagic3) can be semi-automated for high quality, yield, and stable recombinant protein (van Oers, 2011). Many advantages of using BEVs over other expressions systems have been reviewed in previous reports (Airenne et al, 2009(Airenne et al, , 2010Hu, 2005).…”

Section: Baculoviruses and Biomedical Applicationsmentioning

confidence: 99%

“…For instance, in vivo production of antiviral agents including human interferon-γ against influenza virus H1N1 in T. ni larvae have been demonstrated Gomez-Casado et al, 2011). Nevertheless, insect cell cultures and BEVs platforms continue to expand the applications of baculoviruses as novel tools for vaccine development, drug screening, and gene therapy (Airenne et al, 2010(Airenne et al, , 2011Cox & Hollister, 2009, Kost et al, 2005, van Oers, 2011. The recent initiative of having a standard baculovirus reference material repository (BRM) will further boost their application and perhaps hasten the regulatory process for registering new baculovirus products (Kamen et al, 2011).…”

Section: Baculoviruses and Biomedical Applicationsmentioning

confidence: 99%

“…To date, there are different baculovirus-based vaccines for human and veterinary use (van Oers, 2011). Also, vaccines targeting highly pathogenic viruses that are transmitted by arthropod vectors (arboviruses) are being developed (Metz & Pijlman, 2011).…”

Section: Baculoviruses and Vaccine Developmentmentioning

confidence: 99%

See 2 more Smart Citations

Recent Advances in Our Knowledge of Baculovirus Molecular Biology and Its Relevance for the Registration of Baculovirus-Based Products for Insect Pest Population Control

Lapointe¹,

Thumbi²,

Lucarotti³

2012

Integrated Pest Management and Pest Control - Current and Future Tactics

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Antigen generation and display in therapeutic antibody drug discovery – a neglected but critical player

Ebersbach

Geisse

2012

Biotechnology Journal

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Disease intervention by targeting a critical pathway molecule through a blocking antibody or interference by therapeutic proteins is currently en vogue. Generation of blocking antibodies or therapeutic proteins inevitably requires the production of recombinant proteins or cell-based immunogens. Thus, one could call the antigen molecule the neglected player in antibody drug discovery. The variety of methods available for making recombinant proteins or recombinant cell lines that present the target on the cell surface is extensive. These need to be addressed in conjunction with biochemical and biophysical quality criteria and the experimental application intended. Fundamentally, successful production and isolation of monoclonal antibodies requires optimized antigen preparation and presentation to the immune host. This review summarizes the most important aspects of antigen generation and display, enabling logical decision making to give rise to potent high-affinity antibodies.

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Opportunities and challenges for the baculovirus expression system

Cited by 132 publications

References 164 publications

Superinfection Exclusion in Alphabaculovirus Infections Is Concomitant with Actin Reorganization

Superinfection Exclusion in Alphabaculovirus Infections Is Concomitant with Actin Reorganization

Recent Advances in Our Knowledge of Baculovirus Molecular Biology and Its Relevance for the Registration of Baculovirus-Based Products for Insect Pest Population Control

Antigen generation and display in therapeutic antibody drug discovery – a neglected but critical player

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