Opportunity and challenges for nanotechnology application for genome editing in plants

Vats, Sanskriti; Kumawat, Surbhi; Brar, Jashandeep; Kaur, Sukhmandeep; Yadav, Karmveer; Magar, Sayali; Jadhav, P. V.; Salvi, Prafull; Sonah, Humira; Sharma, Sandhya; Deshmukh, Rupesh

doi:10.1016/j.plana.2022.100001

Cited by 20 publications

(5 citation statements)

References 112 publications

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“…However, ensuring biosafety remains a paramount concern in its application. Recent advances in nanotechnology have presented a promising avenue in plants toward enhancing the biosafety of CRISPR/Cas9 technology, creating a potential combination for sustainable genome engineering [57,58]. Nanotechnology can significantly improve the delivery methods of CRISPR/Cas9 components by manipulating matter at the atomic and molecular scale [57].…”

Section: The Promising Role Of Nanotechnology In Enhancing the Biosaf...mentioning

confidence: 99%

CRISPR Variants for Gene Editing in Plants: Biosafety Risks and Future Directions

Movahedi,

Aghaei-Dargiri,

et al. 2023

IJMS

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The CRISPR genome editing technology is a crucial tool for enabling revolutionary advancements in plant genetic improvement. This review shows the latest developments in CRISPR/Cas9 genome editing system variants, discussing their benefits and limitations for plant improvement. While this technology presents immense opportunities for plant breeding, it also raises serious biosafety concerns that require careful consideration, including potential off-target effects and the unintended transfer of modified genes to other organisms. This paper highlights strategies to mitigate biosafety risks and explores innovative plant gene editing detection methods. Our review investigates the international biosafety guidelines for gene-edited crops, analyzing their broad implications for agricultural and biotechnology research and advancement. We hope to provide illuminating and refined perspectives for industry practitioners and policymakers by evaluating CRISPR genome enhancement in plants.

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Section: The Promising Role Of Nanotechnology In Enhancing the Biosaf...mentioning

confidence: 99%

CRISPR Variants for Gene Editing in Plants: Biosafety Risks and Future Directions

Movahedi,

Aghaei-Dargiri,

et al. 2023

IJMS

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“…Gold nanoparticles (AuNP), when compared to other nanoparticles, possess excellent physicochemical stability and excellent biocompatibility. CRISPER–Cas9 modes can be delivered by primarily gold-based nanomaterials and lipid NPs, AuNP and AuNC (Chen et al 2019 ; Vats et al 2022 ).…”

Section: The Dna-free Genome-editing Systemmentioning

confidence: 99%

Strategic transgene-free approaches of CRISPR-based genome editing in plants

et al. 2023

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Genome editing through the alteration of nucleotide sequence has already revolutionized the field of site-directed mutagenesis for a decade. However, research in terms of precision and efficacy in targeting the loci and reduction in off-target mutation has always been a priority when DNA is involved. Therefore, recent research interest lies in utilizing the same precision technology but results in non-transgenic. In this review article, different technological advancements have been explained, which may provide a holistic concept of and need for transgene-free genome editing. The advantage and lacunas of each technology have been critically discussed to deliver a transparent view to the readers. A systematic analysis and evaluation of published research articles implied that researchers across the globe are putting continuous efforts in this direction to eliminate the hindrance of transgenic regulation. Nevertheless, this approach has severe implications legitimate for mitigating the conflict of acceptance, reliability, and generosity of gene-editing technology and sustainably retorting to the expanding global population feeding challenges.

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“…Genome editing is an effective tool for hastening the production of cultivars with enhanced alleles, which have also been approved for distribution as a new variety in the public sector [ 145 ] The CRISPR/Cas9 system allows for precision genome editing in specific genes enabling the generation of genetically engineered crops, and it may be a helpful way for directly changing the genome to improve seed vigor and longevity [ 98 ]. This technique is appropriate for the targeted functional dissection of multiple genes, such as those involved in raffinose production and PLD activity, which are mainly responsible for causing seed destruction or deterioration during long-term storage.…”

Section: Challenges and Future Prospects To Improve Seed Vigor And Lo...mentioning

confidence: 99%

Raffinose family oligosaccharides (RFOs): role in seed vigor and longevity

Salvi

Varshney²,

Majee

2022

Bioscience Reports

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Seed vigor and longevity are important agronomic attributes, as they are essentially associated with crop yield and thus the global economy. Seed longevity is a measure of seed viability and the most essential property in gene bank management since it affects regeneration of seed recycling. Reduced seed life or storability is a serious issue in seed storage since germplasm conservation and agricultural enhancement initiatives rely on it. The irreversible and ongoing process of seed deterioration comprises a complex gene regulatory network and altered metabolism that results in membrane damage, DNA integrity loss, mitochondrial dysregulation, protein damage, and disrupted antioxidative machinery. Carbohydrates and/or sugars, primarily RFOs, have emerged as feasible components for boosting or increasing seed vigor and longevity in recent years. RFOs are known to perform diverse functions in plants, including abiotic and biotic stress tolerance, besides being involved in regulating seed germination, desiccation tolerance, vigor, and longevity. We emphasised and analysed the potential impact of RFOs on seed vigor and longevity in this review. Here, we comprehensively reviewed the molecular mechanisms involved in seed longevity, RFO metabolism, and how RFO content is critical and linked with seed vigor and longevity. Further molecular basis, biotechnological approaches, and CRISPR/Cas applications have been discussed briefly for the improvement of seed attributes and ultimately crop production. Likewise, we suggest advancements, challenges, and future possibilities in this area.

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Opportunity and challenges for nanotechnology application for genome editing in plants

Cited by 20 publications

References 112 publications

CRISPR Variants for Gene Editing in Plants: Biosafety Risks and Future Directions

CRISPR Variants for Gene Editing in Plants: Biosafety Risks and Future Directions

Strategic transgene-free approaches of CRISPR-based genome editing in plants

Raffinose family oligosaccharides (RFOs): role in seed vigor and longevity

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