Optical Absorption and Magnetic Field Effect Based Imaging of Transient Radicals

Beardmore, JP; Antill, Lewis M.; Woodward, Jonathan

doi:10.1002/anie.201502591

Cited by 24 publications

(26 citation statements)

References 13 publications

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“…In our previous paper, 8 we demonstrated the imaging capability and spatial resolution of the microscope. Here we focus on the kinetic and MARY data that can be readily obtained from very small samples, even at low concentrations.…”

Section: Resultsmentioning

confidence: 99%

“…We have already introduced new terms to describe the two different operational modes of the microscope: transient optical absorption detection (TOAD) and magnetic intensity modulation (MIM) imaging. 8 We provide details on the instrumental aspects of these techniques below.…”

Section: Imaging Methodsmentioning

confidence: 99%

“…The preliminary results obtained using our instrument were recently published elsewhere. 8 In this paper, we provide details on our instrumental and experimental approach, demonstrating our technique's capabilities for use with biological samples and describe a methodology which allows the instrument to be used to make measurements on small volumes of samples in which the photochemical processes are non-cyclic, i.e., in which photoexcitation is destructive to the sample.…”

Section: Introductionmentioning

confidence: 99%

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Time-resolved optical absorption microspectroscopy of magnetic field sensitive flavin photochemistry

Antill

Beardmore

Woodward

2018

Review of Scientific Instruments

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The photochemical reactions of blue-light receptor proteins have received much attention due to their very important biological functions. In addition, there is also growing evidence that the one particular class of such proteins, the cryptochromes, may be associated with not only a biological photo-response but also a magneto-response, which may be responsible for the mechanism by which many animals can respond to the weak geomagnetic field. Therefore, there is an important scientific question over whether it is possible to directly observe such photochemical processes, and indeed the effects of weak magnetic fields thereon, taking place both in purified protein samples in vitro and in actual biochemical cells and tissues. For the former samples, the key lies in being able to make sensitive spectroscopic measurements on very small volumes of samples at potentially low protein concentrations, while the latter requires, in addition, spatially resolved measurements on length scales smaller than typical cellular components, i.e., sub-micron resolution. In this work, we discuss a two- and three-color confocal pump-probe microscopic approach to this question which satisfies these requirements and is thus useful for experimental measurements in both cases.

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Section: Resultsmentioning

confidence: 99%

Section: Imaging Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Time-resolved optical absorption microspectroscopy of magnetic field sensitive flavin photochemistry

Antill

Beardmore

Woodward

2018

Review of Scientific Instruments

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“…stimulated Raman scattering (SRS) , two‐photon excited fluorescence ) or pump‐probe microspectroscopy (e.g. transient absorption (TA) ). Conceptually, each of the abovementioned nonlinear microscopic tools provides some specific information on the material properties of the samples investigated: For instance, CARS, SRS or FWM deduce information from vibrational modes of the molecules in the sample and even in cells and tissues of living organisms .…”

Section: Introductionmentioning

confidence: 99%

Transient absorption microscopy: advances in chemical imaging of photoinduced dynamics

Davydova

Cadena

Akimov

et al. 2015

Laser & Photonics Reviews

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This article reviews the state of the art of ultrafast transient absorption microscopy, discusses current experimental concepts and highlights future challenges. The advantages of transient absorption microscopy over other micro-spectroscopic techniques are its high optical resolution combined with high temporal resolution as well as its ability to study non-fluorescent and weakly fluorescent molecular species and to probe excited-state processes. In conventional transient absorption spectroscopy the spectroscopic information usually presents a spatial average over the focal spot of the typically weakly focused probe beam. Transient absorption microscopy, however, enables investigations of the excited state dynamics in individual microscopic areas of a sample. Hence, the technique does not only yield detailed morphological information based on a label-free molecular contrast, but also gives insight into the ultrafast morphology-dependent photoinduced processes in heterogeneous samples. Different variations of transient absorption microscopy have found a number of applications ranging from material sciences to biology, which are discussed in this review together with different setup modifications and approaches towards transient absorption spectroscopy with spatial resolution below the diffraction limit.

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“…[1][2][3] They play an important role in light, oxygen, and voltage-sensitive (LOV) domains sensitive for blue light excitation. [1][2][3] They play an important role in light, oxygen, and voltage-sensitive (LOV) domains sensitive for blue light excitation.…”

mentioning

confidence: 99%

Synthesis and electronic properties of π-extended flavins

et al. 2015

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Flavin derivatives with an extended π-conjugation were synthesized in moderate to good yields from aryl bromides via a Buchwald-Hartwig palladium catalyzed amination protocol, followed by condensation of the corresponding aromatic amines with violuric acid. The electronic properties of the new compounds were investigated by absorption and emission spectroscopy, cyclic voltammetry, density functional theory (DFT) and time dependent density functional theory (TDDFT). The compounds absorb up to 550 nm and show strong luminescence. The photoluminescence quantum yields ϕPL measured in dichloromethane reach 80% and in PMMA (poly(methyl methacrylate)) 77%, respectively, at ambient temperature. The electrochemical redox behaviour of π-extended flavins follows the mechanism previously described for the parent flavin.

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Optical Absorption and Magnetic Field Effect Based Imaging of Transient Radicals

Cited by 24 publications

References 13 publications

Time-resolved optical absorption microspectroscopy of magnetic field sensitive flavin photochemistry

Time-resolved optical absorption microspectroscopy of magnetic field sensitive flavin photochemistry

Transient absorption microscopy: advances in chemical imaging of photoinduced dynamics

Synthesis and electronic properties of π-extended flavins

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