Optical clearing of tissues: Issues of antimicrobial phototherapy and drug delivery

Tuchin, Valery V.; Genina, Elina A.; Tuchina, Elena S.; Svetlakova, Anna V.; Svenskaya, Yulia I.

doi:10.1016/j.addr.2021.114037

Cited by 30 publications

(13 citation statements)

References 433 publications

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“…The phototherapy of wounds is closely related to the problem of infectious disease treatment, especially in the presence of antibiotic-resistant bacteria in the bacterial biofilms covering the wound surface [35,59]. Since the development of antibacterial drugs is not keeping pace with microbial resistance, it is necessary to use alternative antibacterial approaches, including antimicrobial PDT.…”

Section: Discussionmentioning

confidence: 99%

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In Vivo Quantification of the Effectiveness of Topical Low-Dose Photodynamic Therapy in Wound Healing Using Two-Photon Microscopy

et al. 2022

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The effect of low-dose photodynamic therapy on in vivo wound healing with topical application of 5-aminolevulinic acid and methylene blue was investigated using an animal model for two laser radiation doses (1 and 4 J/cm2). A second-harmonic-generation-to-auto-fluorescence aging index of the dermis (SAAID) was analyzed by two-photon microscopy. SAAID measured at 60–80 μm depths was shown to be a suitable quantitative parameter to monitor wound healing. A comparison of SAAID in healthy and wound tissues during phototherapy showed that both light doses were effective for wound healing; however, healing was better at a dose of 4 J/cm2.

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Section: Discussionmentioning

confidence: 99%

“…According to the Arndt-Schultz law, no tissue response will occur when low-level laser light is applied with a low dose. If used with a high dose, it can inhibit tissue response and even induce the proliferation of cancer cells or microorganisms [34,35]. Therefore, there is an optimal dose where a maximal response is obtained [36].…”

Section: Introductionmentioning

confidence: 99%

In Vivo Quantification of the Effectiveness of Topical Low-Dose Photodynamic Therapy in Wound Healing Using Two-Photon Microscopy

et al. 2022

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“…Therefore, we encounter two opposite demands: (1) to make the light irradiation as safe as possible for healthy organs and (2) to deliver the desired light power to targeted PPT sites. The possible solutions are the use of appropriate light waveguides and optical clearing techniques [268][269][270][271].…”

Section: Perspective and Limitationsmentioning

confidence: 99%

Photothermal and Photodynamic Therapy of Tumors with Plasmonic Nanoparticles: Challenges and Prospects

Bucharskaya

Khlebtsov

et al. 2022

Materials

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Cancer remains one of the leading causes of death in the world. For a number of neoplasms, the efficiency of conventional chemo- and radiation therapies is insufficient because of drug resistance and marked toxicity. Plasmonic photothermal therapy (PPT) using local hyperthermia induced by gold nanoparticles (AuNPs) has recently been extensively explored in tumor treatment. However, despite attractive promises, the current PPT status is limited by laboratory experiments, academic papers, and only a few preclinical studies. Unfortunately, most nanoformulations still share a similar fate: great laboratory promises and fair preclinical trials. This review discusses the current challenges and prospects of plasmonic nanomedicine based on PPT and photodynamic therapy (PDT). We start with consideration of the fundamental principles underlying plasmonic properties of AuNPs to tune their plasmon resonance for the desired NIR-I, NIR-2, and SWIR optical windows. The basic principles for simulation of optical cross-sections and plasmonic heating under CW and pulsed irradiation are discussed. Then, we consider the state-of-the-art methods for wet chemical synthesis of the most popular PPPT AuNPs such as silica/gold nanoshells, Au nanostars, nanorods, and nanocages. The photothermal efficiencies of these nanoparticles are compared, and their applications to current nanomedicine are shortly discussed. In a separate section, we discuss the fabrication of gold and other nanoparticles by the pulsed laser ablation in liquid method. The second part of the review is devoted to our recent experimental results on laser-activated interaction of AuNPs with tumor and healthy tissues and current achievements of other research groups in this application area. The unresolved issues of PPT are the significant accumulation of AuNPs in the organs of the mononuclear phagocyte system, causing potential toxic effects of nanoparticles, and the possibility of tumor recurrence due to the presence of survived tumor cells. The prospective ways of solving these problems are discussed, including developing combined antitumor therapy based on combined PPT and PDT. In the conclusion section, we summarize the most urgent needs of current PPT-based nanomedicine.

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“…PDT is a photochemistry-based treatment that combines photosensitizers, light, and oxygen. When the photosensitizer is activated by the light of a specific wavelength, local reactive oxygen species (ROS) are elevated to destroy the proteins, nucleic acids, and membranes of the cells, and eventually leads to cell death [8][9][10]. Clinically, PDT is used to treat a variety of diseases, including cancer, acne, psoriasis, and bacterial infections [8,11,12].…”

Section: Introductionmentioning

confidence: 99%

“…When the photosensitizer is activated by the light of a specific wavelength, local reactive oxygen species (ROS) are elevated to destroy the proteins, nucleic acids, and membranes of the cells, and eventually leads to cell death [8][9][10]. Clinically, PDT is used to treat a variety of diseases, including cancer, acne, psoriasis, and bacterial infections [8,11,12]. In animal and pre-clinic studies, there are numerus studies that applied PDT to treat infected wounds, which are a common occurrence in clinics [9,11].…”

Section: Introductionmentioning

confidence: 99%

A Combined Cyanine/Carbomer Gel Enhanced Photodynamic Antimicrobial Activity and Wound Healing

et al. 2022

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As a non-invasive and non-specific therapeutic approach, photodynamic therapy (PDT) has been used to treat antibiotic-resistant bacteria with encouraging efficacy. Inspired by light, the photosensitizers can produce excessive reactive oxygen species (ROS) and, thus, effectively destroy or kill bacteria. Cyanine (Cy), a traditional photosensitizer for PDT, has the advantages of low cytotoxicity and high ROS yield. Yet, the water solubility and photostability for Cy are poor, which substantially limit its antibacterial efficiency and clinical translation. Herein, we combined Cy with carbomer gel (CBMG) to form a photodynamic Cy-CBMG hydrogel. In this system, Cy was evenly dispersed in CBMG, and CBMG significantly improved the water solubility and photostability of Cy via electrostatic interactions. The developed Cy-CBMG gel had less photodegradation under laser irradiation and thus can effectively elevate ROS accumulation in bacteria. The Cy-CBMG compound presented remarkable ROS-induced killing efficacy against methicillin-resistant Staphylococcus aureus (93.0%) and extended-spectrum β-lactamase-producing Escherichia coli (88.7%) in vitro. Moreover, as a potential wound dressing material, the Cy-CBMG hydrogel exhibited excellent biocompatibility and effective antimicrobial ability to promote wound healing in vivo. Overall, this work proposed a practical strategy to synthesize a photosensitizer–excipient compound to enhance the photophysical property and antibacterial efficacy for PDT.

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Optical clearing of tissues: Issues of antimicrobial phototherapy and drug delivery

Cited by 30 publications

References 433 publications

In Vivo Quantification of the Effectiveness of Topical Low-Dose Photodynamic Therapy in Wound Healing Using Two-Photon Microscopy

In Vivo Quantification of the Effectiveness of Topical Low-Dose Photodynamic Therapy in Wound Healing Using Two-Photon Microscopy

Photothermal and Photodynamic Therapy of Tumors with Plasmonic Nanoparticles: Challenges and Prospects

A Combined Cyanine/Carbomer Gel Enhanced Photodynamic Antimicrobial Activity and Wound Healing

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