Optical coding of mammalian cells using semiconductor quantum dots

Mattheakis, Larry; Dias, Jennifer; Choi, Yun-Shik; Gong, Junli; Bruchez, Marcel P.; Liu, Jianquan; Wang, Eugene

doi:10.1016/j.ab.2004.01.031

Cited by 200 publications

(148 citation statements)

References 35 publications

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“…Os NC são marcadores fluorescentes, constituídos de materiais semi-condutores, resistentes e emissores de luz, pois mesmo medindo cerca de 2 a 6nm podem ser facilmente detectados por microscopia de fluorescência ou citometria de fluxo (MATTHEAKIS et al, 2004).…”

Section: Introductionunclassified

Transplante autólogo de células mononucleares da medula óssea em úlcera de córnea experimental em cães

et al. 2008

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Section: Introductionunclassified

Transplante autólogo de células mononucleares da medula óssea em úlcera de córnea experimental em cães

et al. 2008

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“…The key to live-cell observation of QD-labeled structures inside cells is obviously an efficient mechanism of QD uptake. Because QDs are not able to permeate cell membranes in the same way as standard organic dyes, various methods have been implemented to introduce them into cells, including injection or microinjection (Dubertret et al 2002;Larson et al 2003), the use of transfection agents (Chang et al 2008), liposome-or electroporation-mediated incorporation (Derfus et al 2004) or peptide-based and/or aptamer-mediated delivery (Chang et al 2008;Mattheakis et al 2004;Zhang et al 2006). In the final approach, exciting developments in the delivery of large molecules by aptamers and carrier peptide conjugates have been reported (Ho and Leong 2010;Walther et al 2008).…”

Section: Quantum Dotsmentioning

confidence: 99%

Multi-dimensional correlative imaging of subcellular events: combining the strengths of light and electron microscopy

Nykanen

Jahn

et al. 2010

Biophys Rev

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To genuinely understand how complex biological structures function, we must integrate knowledge of their dynamic behavior and of their molecular machinery. The combined use of light or laser microscopy and electron microscopy has become increasingly important to our understanding of the structure and function of cells and tissues at the molecular level. Such a combination of two or more different microscopy techniques, preferably with different spatial-and temporal-resolution limits, is often referred to as 'correlative microscopy'. Correlative imaging allows researchers to gain additional novel structurefunction information, and such information provides a greater degree of confidence about the structures of interest because observations from one method can be compared to those from the other method(s). This is the strength of correlative (or 'combined') microscopy, especially when it is combined with combinatorial or non-combinatorial labeling approaches. In this topical review, we provide a brief historical perspective of correlative microscopy and an in-depth overview of correlative sample-preparation and imaging methods presently available, including future perspectives on the trend towards integrative microscopy and microanalysis.

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“…43 Cationic lipid-capped QD delivery into tumor cells was successfully demonstrated by Voura et al 40 as was peptide-mediated delivery of QDs into Chinese hamster ovarian cells. 42 Cationic POSS conjugated to BODIPY was seen to be taken up by cells with a uniform cytoplasmic distribution. 44 Although the M-POSS used to coat our QDs is not the cationic form, it is likely that the amphiphillicity that it imparts to the QD structure has a role in its increased intracellular uptake across the lipophilic cell membranes.…”

mentioning

confidence: 99%

“…It is possible that the mechanism of QD uptake for POSS-QDs differs from MSA QDs such that the former are rapidly taken up by pathways other than nonspecific endocytosis, leading to increased QD concentration within the cells and higher intracellular fluorescence. It has been previously demonstrated that QD uptake by cells can be enhanced by conjugating the QDs to cationic lipids or peptides [39][40][41][42] or amphiphilic proteins. 43 Cationic lipid-capped QD delivery into tumor cells was successfully demonstrated by Voura et al 40 as was peptide-mediated delivery of QDs into Chinese hamster ovarian cells.…”

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confidence: 99%

A novel POSS-coated quantum dot for biological application

Rizvi¹,

Yildirimer²,

Ghaderi³

et al. 2012

IJN

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Quantum dots (QDs) are fluorescent semiconductor nanocrystals that have the potential for major advancements in the field of nanomedicine through their unique photophysical properties. They can potentially be used as fluorescent probes for various biomedical imaging applications, including cancer localization, detection of micrometastasis, image guided surgery, and targeted drug delivery. Their main limitation is toxicity, which requires a biologically compatible surface coating to shield the toxic core from the surrounding environment. However, this leads to an increase in QD size that may lead to problems of excretion and systemic sequestration. We describe a one pot synthesis, characterization, and in vitro cytotoxicity of a novel polyhedral oligomeric silsesquioxane (POSS)-coated CdTe-cored QD using mercaptosuccinic acid (MSA) and D-cysteine as stabilizing agents. Characterization was performed using transmission electron microscopy Fourier transform infrared spectroscopy, and photoluminescence studies. POSS-coated QDs demonstrated high colloidal stability and enhanced photostability on high degrees of ultraviolet (UV) excitation compared to QDs coated with MSA and D-cysteine alone (P value , 0.05). In vitro toxicity studies showed that both POSS and MSA-QDs were significantly less toxic than ionized salts of Cd +2 and Te −2 . Confocal microscopy confirmed high brightness of POSS-QDs in cells at both 1 and 24 hours, indicating that these QDs are rapidly taken up by cells and remain photostable in a biological environment. We therefore conclude that a POSS coating confers biological compatibility, photostability, and colloidal stability while retaining the small size and unique photophysical properties of the QDs. The amphiphilic nature of the coating allows solubility in aqueous solutions and rapid transfer across cell membranes, enabling the use of lower concentrations of the QDs for an overall reduced toxicity particularly for prolonged live cell and in vivo imaging applications.

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Optical coding of mammalian cells using semiconductor quantum dots

Cited by 200 publications

References 35 publications

Transplante autólogo de células mononucleares da medula óssea em úlcera de córnea experimental em cães

Transplante autólogo de células mononucleares da medula óssea em úlcera de córnea experimental em cães

Multi-dimensional correlative imaging of subcellular events: combining the strengths of light and electron microscopy

A novel POSS-coated quantum dot for biological application

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