Optical detection of nanoparticle agglomeration in a living system under the influence of a magnetic field

Müller, Robert N.; Stránik, Ondrej; Schlenk, Florian; Werner, Sebastian; Malsch, Daniéll; Fischer, Dagmar; Fritzsche, Wolfgang

doi:10.1016/j.jmmm.2014.10.043

Cited by 11 publications

(8 citation statements)

References 13 publications

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“…11b and c show the transport of particles in the direction of low and high magnetic eld intensities (0.012 and 0.019 T). 51 When no MF was applied to the particles, the particles were observed to be polydispersed in the uoride solution as shown in Fig. 11d.…”

Section: Effect Of Initial Concentrationmentioning

confidence: 98%

Removal of fluoride ions using a polypyrrole magnetic nanocomposite influenced by a rotating magnetic field

et al. 2020

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Section: Effect Of Initial Concentrationmentioning

confidence: 98%

Removal of fluoride ions using a polypyrrole magnetic nanocomposite influenced by a rotating magnetic field

et al. 2020

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“…Optimization is still required to reduce the amount of nanoparticles. Müller et al [ 75 ] investigated the transport of magnetic particles in vessels of hen’s egg models. The flow was subjected to the influence of a magnetic field in dark field reflected light and fluorescence mode.…”

Section: An Overview Of Image Analysis Methods For Microfluidic Blmentioning

confidence: 99%

Manual and Automatic Image Analysis Segmentation Methods for Blood Flow Studies in Microchannels

et al. 2021

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In blood flow studies, image analysis plays an extremely important role to examine raw data obtained by high-speed video microscopy systems. This work shows different ways to process the images which contain various blood phenomena happening in microfluidic devices and in microcirculation. For this purpose, the current methods used for tracking red blood cells (RBCs) flowing through a glass capillary and techniques to measure the cell-free layer thickness in different kinds of microchannels will be presented. Most of the past blood flow experimental data have been collected and analyzed by means of manual methods, that can be extremely reliable, but they are highly time-consuming, user-intensive, repetitive, and the results can be subjective to user-induced errors. For this reason, it is crucial to develop image analysis methods able to obtain the data automatically. Concerning automatic image analysis methods for individual RBCs tracking and to measure the well known microfluidic phenomena cell-free layer, two developed methods are presented and discussed in order to demonstrate their feasibility to obtain accurate data acquisition in such studies. Additionally, a comparison analysis between manual and automatic methods was performed.

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“…Superparamagnetic iron oxide nanoparticles (SPIONs) have been proposed as magnetic imaging agents but can be affected by external magnetic fields. Intravital microscopy of fluorescently labeled starch-coated SPIONs demonstrated steady flow within the CAM vasculature in the absence of a magnetic field . When a magnetic field was added, agglomeration and reduced velocity of flow were readily apparent.…”

Section: The Chick Embryo and Cam For Assessing Therapeutic Efficacy ...mentioning

confidence: 99%

Bridging the In Vitro to In Vivo gap: Using the Chick Embryo Model to Accelerate Nanoparticle Validation and Qualification for In Vivo studies

2022

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We postulate that nanoparticles (NPs) for use in therapeutic applications have largely not realized their clinical potential due to an overall inability to use in vitro results to predict NP performance in vivo. The avian embryo and associated chorioallantoic membrane (CAM) has emerged as an in vivo preclinical model that bridges the gap between in vitro and in vivo, enabling rapid screening of NP behavior under physiologically relevant conditions and providing a rapid, accessible, economical, and more ethical means of qualifying nanoparticles for in vivo use. The CAM is highly vascularized and mimics the diverging/converging vasculature of the liver, spleen, and lungs that serve as nanoparticle traps. Intravital imaging of fluorescently labeled NPs injected into the CAM vasculature enables immediate assessment and quantification of nano-bio interactions at the individual NP scale in any tissue of interest that is perfused with a microvasculature. In this review, we highlight how utilization of the avian embryo and its CAM as a preclinical model can be used to understand NP stability in blood and tissues, extravasation, biocompatibility, and NP distribution over time, thereby serving to identify a subset of NPs with the requisite stability and performance to introduce into rodent models and enabling the development of structure–property relationships and NP optimization without the sacrifice of large populations of mice or other rodents. We then review how the chicken embryo and CAM model systems have been used to accelerate the development of NP delivery and imaging agents by allowing direct visualization of targeted (active) and nontargeted (passive) NP binding, internalization, and cargo delivery to individual cells (of relevance for the treatment of leukemia and metastatic cancer) and cellular ensembles (e.g., cancer xenografts of interest for treatment or imaging of cancer tumors). We conclude by showcasing emerging techniques for the utilization of the CAM in future nano-bio studies.

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Optical detection of nanoparticle agglomeration in a living system under the influence of a magnetic field

Cited by 11 publications

References 13 publications

Removal of fluoride ions using a polypyrrole magnetic nanocomposite influenced by a rotating magnetic field

Removal of fluoride ions using a polypyrrole magnetic nanocomposite influenced by a rotating magnetic field

Manual and Automatic Image Analysis Segmentation Methods for Blood Flow Studies in Microchannels

Bridging the In Vitro to In Vivo gap: Using the Chick Embryo Model to Accelerate Nanoparticle Validation and Qualification for In Vivo studies

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