Optical immunoprobe development for multiresidue monitoring in water

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

“…Thus the signal decreases as the analyte concentration increases. Binding inhibition assay is an alternative test format of competitive assay, with the antigens being immobilized and the antibodies being labeled [39,40]. In the sandwich assay [41][42][43], antibodies to analytes are first immobilized to a sensor.…”

TIRF Array Biosensor for Environmental Monitoring

“…The most commonly used lasers are the argon-ion ( 488 nm) laser for fluorescein and a helium-neon (633 nm) or diode laser (635 nm) for the cyanine dye (Cy5). A number of devices have been used in the detection of the resulting fluorescence emission, in particular CCD cameras [70][71][72], photomultiplier tubes (PMT) [73], photodiodes [74], a single photomultiplier tube [75,76] and more recently a complementary metal oxide-semiconductor (CMOS) camera [77].…”

Peer Reviewed: Can Affinity Sensors Be Used To Detect Food Contaminants?

“…Fluorescence sensors of the inhibition type (Figure 2b) have been used for determining atrazine, simazine, and 2,4-D (22,23,37,38). The respective analyte was immobilized on an optical fiber or planar waveguide, either directly or via an aminodextran matrix (23).…”

“…Because antibodies usually recognize only a small number of compounds (e.g., the primary analyte and cross-reactants), multiresidue systems, based on a combination of several antibody systems, have also been used. Configurations have included the serial arrangement of flow-through cells and the spatially resolved immobilization of binding partners on planar waveguides (23,38,39). The latter approach leads to more compact systems.…”