Optical immunosensing systems — are they meeting market needs?

Robinson, G.A.

doi:10.1016/0956-5663(93)80072-w

Cited by 3 publications

(1 citation statement)

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“…The photons, emitted at angles smaller than the critical angle, are trapped in the capillary walls and the light leaving the capillary is detected and the level of photons correlated to the concentration of the analyte. Sensitivities of 3 ng mL -1 and 0.5 ng mL -1 have been obtained for hCG in serum and estrone-3-glucuronide in urine, respectively [101,102,103]. More recently, Petrou et al [104] developed a similar system to simultaneously determine the level of three hormones, follitropin, hCG and prolactin, in human serum, which levels are important for the diagnosis of fertility and development disorders.…”

Section: Indirect Optical Detectionmentioning

confidence: 99%

Trends and Perspectives in Immunosensors

2012

Antibodies Applications and New Developments

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Immunosensors are devices that comprise both a biomolecular recognition system, such as an antibody and its corresponding antigen, and a transducer to translate the high affinity and specific binding event into a physical signal.Antibodies are produced by an immunological response to the presence of a foreign substance called an antigen. Antibodies may be immobilised onto a variety of platforms including bulk planar surfaces and nanoparticles by either covalent or adsorption strategies. Different interfaces between the bio-components and the detector are available to monitor in 'real-time' the signal generated by biological interactions. The transducers detect, for example, the change in electron transfer, absorbance, fluorescence, refractive index, mass change or heat transfer as the antibody binds to the antigen of interest. Such analytical devices have allowed a wide range of analytes to be identified and quantified such as pathogens, toxins, environmental food contaminants and disease biomarkers.The demand for sensitive, rapid, 'on-site' techniques has taken advantage of the latest advances in microfluidics and nanotechnology. This chapter will highlight current trends in immobilisation, micro/nano-fluidics/ and transducers utilised. A number of examples outlining the exploitation of these elements in immunosensors and their successful applications will be described.

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