Optical neuronal guidance in three-dimensional matrices

Graves, Catherine E.; McAllister, Ryan; Rosoff, William J.; Urbach, Jeffrey S.

doi:10.1016/j.jneumeth.2009.02.004

Cited by 21 publications

(22 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The period of TLE exposure to LLLS and the beam laser characteristics are comparable to the previous reports on in vitro neuronal stimulation [17, 18]. …”

Section: Resultssupporting

confidence: 84%

Near-infrared low-level laser stimulation of telocytes from human myometrium

et al. 2014

View full text Add to dashboard Cite

Telocytes (TCs) are a brand-new cell type frequently observed in the interstitial space of many organs (see www.telocytes.com). TCs are defined by very long (tens of micrometers) and slender prolongations named telopodes. At their level, dilations—called podoms (~300 nm), alternate with podomers (80–100 nm). TCs were identified in a myometrial interstitial cell culture based on morphological criteria and by CD34 and PDGF receptor alpha (PDGFRα) immunopositivity. However, the mechanism(s) of telopodes formation and/or elongation and ramification is not known. We report here the low-level laser stimulation (LLLS) using a 1,064-nm neodymium-doped yttrium aluminum garnet (Nd:YAG) laser (with an output power of 60 mW) of the telopodal lateral extension (TLE) growth in cell culture. LLLS of TCs determines a higher growth rate of TLE in pregnant myometrium primary cultures (10.3 ± 1.0 μm/min) compared to nonpregnant ones (6.6 ± 0.9 μm/min). Acute exposure (30 min) of TCs from pregnant myometrium to 1 μM mibefradil, a selective inhibitor of T-type calcium channels, determines a significant reduction in the LLLS TLE growth rate (5.7 ± 0.8 μm/min) compared to LLLS per se in same type of samples. Meanwhile, chronic exposure (24 h) completely abolishes the LLLS TLE growth in both nonpregnant and pregnant myometria. The initial direction of TLE growth was modified by LLLS, the angle of deviation being more accentuated in TCs from human pregnant myometrium than in TCs from nonpregnant myometrium. In conclusion, TCs from pregnant myometrium are more susceptible of reacting to LLLS than those from nonpregnant myometrium. Therefore, some implications are emerging for low-level laser therapy (LLLT) in uterine regenerative medicine.

show abstract

“…The period of TLE exposure to LLLS and the beam laser characteristics are comparable to the previous reports on in vitro neuronal stimulation [17, 18]. …”

Section: Resultssupporting

confidence: 84%

Near-infrared low-level laser stimulation of telocytes from human myometrium

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Previous estimates of this effect in the optical neuronal guidance experiments suggested a negligible temperature increase (Albrecht-Buehler, 1991;Carnegie et al, 2008Carnegie et al, , 2009Ehrlicher et al, 2002;Graves et al, 2009;Higuchi et al, 2005Higuchi et al, , 2007Mohanty et al, 2005;Stevenson et al, 2006;Mathew et al, 2010;Koch et al, 2004), but our more detailed simulations show a temperature increase of the order 1 • C/100 mW of laser power, which is in agreement with experimental results and modelling from the field of optical trapping (Schönle and Hell, 1998;Braun and Libchaber, 2002;Peterman et al, 2003;Ebert et al, 2007). Furthermore, we find a temperature gradient of the order 1 • C/typical neuronal growth cone-radius.…”

Section: Introductionsupporting

confidence: 90%

“…A curious new addition to the field of neuronal guidance is optical neuronal guidance (Carnegie et al, 2008(Carnegie et al, , 2009Ehrlicher et al, 2002;Graves et al, 2009;Higuchi et al, 2005Higuchi et al, , 2007Mohanty et al, 2005;Stevenson et al, 2006;Mathew et al, 2010;Koch et al, 2004) reviewed in Franze and Guck (2010) and Stevenson et al (2010). In 1991 it was first observed that Swiss 3T3 cells (mammalian fibroblast-like cells) would extend pseudopodia towards near-infrared light sources (Albrecht-Buehler, 1991), and in 2002 it was shown that a near-infrared laser spot placed on and in front of the leading edge of growing PC12 and NG108 neurons enhanced the neuronal growth into the laser spot and resulted in laser-guided turns of the growth cone and increased growth speed (Ehrlicher et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Analysis of laser-induced heating in optical neuronal guidance

Ebbesen

Bruus

2012

Journal of Neuroscience Methods

View full text Add to dashboard Cite

a b s t r a c tRecently, it has been shown that it is possible to control the growth direction of neuronal growth cones by stimulation with weak laser light; an effect dubbed optical neuronal guidance. The effect exists for a broad range of laser wavelengths, spot sizes, spot intensities, optical intensity profiles and beam modulations, but it is unknown which biophysical mechanisms govern it. Based on thermodynamic modeling and simulation using published experimental parameters as input, we argue that the guidance is linked to heating. Until now, temperature effects due to laser-induced heating of the guided neuron have been neglected in the optical neuronal guidance literature. The results of our finite-element-method simulations show the relevance of the temperature field in optical guidance experiments and are consistent with published experimental results and modeling in the field of optical traps. Furthermore, we propose two experiments designed to test this hypotheses experimentally. For one of these experiments, we have designed a microfluidic platform, to be made using standard microfabrication techniques, for incubation of neurons in temperature gradients on micrometer lengthscales.

show abstract

“…The Gaussian profile has been the most commonly used in the literature for successful optical guided neuronal growth [1,3,4,36]. Figure 5 is an image sequence showing a NG-108 growth cone being guided by a focused Gaussian spot of approximate power 40 mW and spot size 1 mm.…”

Section: Gaussian Profilementioning

confidence: 99%

Automated laser guidance of neuronal growth cones using a spatial light modulator

Carnegie

Čižmár

Baumgartl

et al. 2009

Journal of Biophotonics

View full text Add to dashboard Cite

The growth cone of a developing neuron can be guided using a focused infra-red (IR) laser beam [1]. In previous setups this process has required a significant amount of user intervention to adjust continuously the laser beam to guide the growing neuron. Previously, a system using an acousto-optical deflector (AOD) has been developed to steer the beam [2]. However, to enhance the controllability of this system, here we demonstrate the use of a computer controlled spatial light modulator (SLM) to steer and manipulate the shape of a laser beam for use in guided neuronal growth. This new experimental setup paves the way to enable a comprehensive investigation into beam shaping effects on neuronal growth and we show neuronal growth initiated by a Bessel light mode. This is a robust platform to explore the biochemistry of this novel phenomenon.

show abstract

Optical neuronal guidance in three-dimensional matrices

Cited by 21 publications

References 26 publications

Near-infrared low-level laser stimulation of telocytes from human myometrium

Near-infrared low-level laser stimulation of telocytes from human myometrium

Analysis of laser-induced heating in optical neuronal guidance

Automated laser guidance of neuronal growth cones using a spatial light modulator

Contact Info

Product

Resources

About