Optical Properties and Nondestructive Estimation of Anthocyanin Content in Plant Leaves¶

Gitelson, Anatoly A.; Merzlyak, Mark N.; Чивкунова, О. Б.

doi:10.1562/0031-8655(2001)074<0038:opaneo>2.0.co;2

Cited by 753 publications

(194 citation statements)

References 20 publications

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“…For the extraction of chlorophylls and carotenoids, the above powder was extracted in 80% acetone in black bottles and vacuum filtered; absorbance of the extract was determined immediately using a spectrophotometer and quantities determined as described by Gitelson et al (2001). Specific absorption coefficients of chlorophylls and carotenoids were used as reported by Lichtenthaler (1987).…”

Section: Determination Of Metabolite Levelsmentioning

confidence: 99%

Physiological implications of metabolite biosynthesis for net assimilation and heat-stress tolerance of sugarcane ( Saccharum officinarum) sprouts

2006

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Global increase in ambient temperature is a critical factor for plant growth. In order to study the changes in growth over short intervals, various primary and secondary metabolites, and their relationships with thermotolerance, 1-month-old sugarcane (Saccharum officinarum) sprouts were grown under control conditions (28 degrees C) or under heat-stress conditions (40 degrees C), and measurements were made at six 12-h intervals. Heat stress greatly reduced dry matter and leaf area of sprouts initially but only nominally later on. Changes in the rates of relative growth and net assimilation were greater than relative leaf expansion, indicating an adverse effect of heat on assimilation of nutrients and CO(2) in producing dry matter. Although reduction in leaf water potential was an immediate response to heat, this effect was offset by early synthesis of free proline, glycinebetaine and soluble sugars (primary metabolites). Among secondary metabolites, anthocyanin synthesis was similar to primary metabolites; carotenoids and soluble phenolics accumulated later while chlorophyll remained unaffected. Relationships of growth attributes and metabolite levels, not seen in the controls, were evident under heat stress. In summary, observed changes in metabolite levels were spread over time and space and were crucial in improving net assimilation and heat tolerance of sugarcane.

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Section: Determination Of Metabolite Levelsmentioning

confidence: 99%

Physiological implications of metabolite biosynthesis for net assimilation and heat-stress tolerance of sugarcane ( Saccharum officinarum) sprouts

2006

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“…A molecular weight of 570 for carotenoids was used for calculations. Anthocyanin content was determined following extract acidiWcation with concentrated HCl, and an absorption coeYcient of 30 mM -1 cm ¡1 at 530 nm was used according to Gitelson et al (2001).…”

Section: Pigment Determinationmentioning

confidence: 99%

α-Tocopherol may influence cellular signaling by modulating jasmonic acid levels in plants

Munné‐Bosch

Weiler

Alegre

et al. 2006

Planta

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Most studies on the function of tocopherols in plants have focused on their photo-protective and antioxidant properties, and it has been recently suggested, though not yet demonstrated, that they may also play a role in cellular signaling. By using vte1 mutants of Arabidopsis thaliana, with an insertion in the promoter region of the gene encoding tocopherol cyclase, we demonstrate here for the first time that tocopherol deficiency may alter endogenous phytohormone levels in plants, thereby reducing plant growth and triggering anthocyanin accumulation in leaves. In plants grown under a combination of high light and low temperature conditions to induce anthocyanin accumulation, we evaluated age-dependent changes in tocopherols, indicators of photo-oxidative stress, phytohormone levels, plant growth and anthocyanin levels in wild type and vte1 mutants. These mutants showed lower tocopherol levels, reduced growth and enhanced anthocyanin accumulation compared with the wild type, while both the maximum and relative efficiencies of PSII, chlorophylls, and carotenoids were not significantly altered. Analyses of phytohormone levels revealed that reduced growth and enhanced anthocyanin accumulation in tocopherol-deficient plants were preceded by increased jasmonic acid levels. This is the first study suggesting a direct effect of tocopherols on phytohormones levels in plants and will undoubtedly help us to better understand the multiple functions tocopherols play in plants, as well as the cellular signaling mechanisms responsible for the phenotypes thus far described in tocopherol-deficient plants.

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“…In contrast with Chls, more yellow-green radiation is absorbed by anthocyanins (Gamon and Surfus 1999, Neill and Gould 1999, Merzlyak and Chivkunova 2000, Gitelson et al 2001. Therefore, under measuring WR compared with RR, the existence of anthocyanins would interfere with comparison of photosynthesis and Chl fluorescence between red and green leaves.…”

Section: Introductionmentioning

confidence: 99%

Co<sub>2</sub> assimilation and chlorophyll fluorescence in green versus red Berberis thunbergii leaves measured with different quality irradiation

Li¹,

Cheng²,

Peng³

et al. 2009

Photosynt.

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Photosynthesis, photorespiration, and chlorophyll (Chl) fluorescence in green and red Berberis thunbergii leaves were studied with two different measuring radiations, red (RR) and "white" (WR). The photosynthetic and photorespiration rates responded differently to the different radiation qualities, which indicate that the carboxylase and oxygenase activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) were affected. Differences in photosynthetic rate between the two color leaves were less under RR than under WR. However, this reduced difference in photosynthetic rate was not correlated with the stomatal response to the measuring radiation qualities. Compared with the WR, the RR reduced the differences in dark-adapted minimum and maximum fluorescence, steady-state fluorescence, light-adapted maximum fluorescence, and actual photochemical efficiency (Φ PS2 ) of photosystem 2 (PS2), but enlarged the difference in non-photochemical quenching between the two color leaves. Differences in both maximum quantum yield of PS2 and ratio of Φ PS2 to quantum yield of CO 2 fixation between the two color leaves were similar under the two measuring radiations. To exclude disturbance of radiation attenuation caused by anthocyanins, it is better to use RR to compare the photosynthesis and Chl fluorescence in green versus red leaves.

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Optical Properties and Nondestructive Estimation of Anthocyanin Content in Plant Leaves¶

Cited by 753 publications

References 20 publications

Physiological implications of metabolite biosynthesis for net assimilation and heat-stress tolerance of sugarcane ( Saccharum officinarum) sprouts

Physiological implications of metabolite biosynthesis for net assimilation and heat-stress tolerance of sugarcane ( Saccharum officinarum) sprouts

α-Tocopherol may influence cellular signaling by modulating jasmonic acid levels in plants

Co<sub>2</sub> assimilation and chlorophyll fluorescence in green versus red Berberis thunbergii leaves measured with different quality irradiation

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