Optical Spectroscopy Study of the Interaction Between Quercetin and Human Serum Albumin

Wybranowski, Tomasz; Kruszewski, Stefan

doi:10.12693/aphyspola.125.a-57

Cited by 8 publications

(4 citation statements)

References 13 publications

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“…However, TGA analysis was failed to calculate the accurate drug-loading rate, since Quercetin was decomposed slowly over a wide temperature range. What’s worse, in vitro drug-release study was also not successful due to the fact that the Quercetin was hard to dissolve in PBS and easy to oxidize [ 325 ]. Cell experiments indicated that the drug-loaded COF successfully killed most of human breast carcinoma MDA-MB-231 cells while the bare COFs showed no cytotoxicity.…”

Section: Cofs For Biomedical Applicationsmentioning

confidence: 99%

Covalent Organic Frameworks: From Materials Design to Biomedical Application

et al. 2017

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Covalent organic frameworks (COFs) are newly emerged crystalline porous polymers with well-defined skeletons and nanopores mainly consisted of light-weight elements (H, B, C, N and O) linked by dynamic covalent bonds. Compared with conventional materials, COFs possess some unique and attractive features, such as large surface area, pre-designable pore geometry, excellent crystallinity, inherent adaptability and high flexibility in structural and functional design, thus exhibiting great potential for various applications. Especially, their large surface area and tunable porosity and π conjugation with unique photoelectric properties will enable COFs to serve as a promising platform for drug delivery, bioimaging, biosensing and theranostic applications. In this review, we trace the evolution of COFs in terms of linkages and highlight the important issues on synthetic method, structural design, morphological control and functionalization. And then we summarize the recent advances of COFs in the biomedical and pharmaceutical sectors and conclude with a discussion of the challenges and opportunities of COFs for biomedical purposes. Although currently still at its infancy stage, COFs as an innovative source have paved a new way to meet future challenges in human healthcare and disease theranostic.

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Section: Cofs For Biomedical Applicationsmentioning

confidence: 99%

Covalent Organic Frameworks: From Materials Design to Biomedical Application

et al. 2017

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“…The amount of quercetin entrapped in liposomes was determined through the indirect method based on the measurement of the drug present in the external phase [ 22 ]. After the centrifugation process in the liposomes preparation, the supernatant containing free drug was analyzed for the drug content via double beam UV-VIS spectrophotometer (Shanghai Mapada Instruments UV-6100, China) at 268 nm and 375 nm where the quercetin exhibits two major absorption peaks [ 23 ]. Although the absorbance values at both wavelengths were recorded, data collected at 268 nm was used in all calculations due to high consistency.…”

Section: Methodsmentioning

confidence: 99%

PEG4000 modified liposomes enhance the solubility of quercetin and improve the liposome functionality: in vitro characterization and the cellular efficacy

DEMİRBOLAT¹,

ERDOĞAN²,

COŞKUN³

et al. 2022

Turkish Journal of Chemistry

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Quercetin, a multifunctional therapeutic agent, is used in various types of cancer. However, its therapeutic effect is limited by virtue of poorly aqueous solubility and instability in the physiological medium. To overcome these limitations, we aimed (i) to design quercetin loaded liposomes with unlinked-PEG4000 with regard to not only surface modification but also solubility enhancement, and (ii) to investigate the antineoplastic effects on HeLa cells. PEG4000 increased the quercetin solubility 2.2 fold. PEG4000 modified liposomes displayed small particle size (254 ± 69 nm), low polydispersity index (0.236 ± 0.018), favorable zeta potential (–35.4 ± 0.6 mV), high quercetin encapsulation efficiency (87.6 ± 5.6%), and drug loading (22.2 ± 6.9%). The homogeneity and particle size of stable PEGylated liposomes were proved by transmission electron microscopy. The drug release was reached up to 65.1 ± 3.8% in 6 h. The IC 50 value of quercetin loaded PEGylated liposomes was 16.3 μg/mL on HeLa cells, while that of quercetin was 88.3 μg/mL. PEGylated liposomes remarkably hampered the adherence and colony formation ability of cells according to crystal violet staining tests. The convenience of PEGylated liposomes for the parenteral application was stated by the hemolysis assay. The high-throughput screening assays based on AO/PI staining proved the drastic decrease of viable cell count. Moreover, qPCR tests based on gene expression levels revealed that the quercetin loaded PEGylated liposomes treatment could be more effective than free quercetin on the mitochondrial apoptosis of HeLa cells. These promising results allow considering further in vivo studies for efficient cancer treatment with quercetin loaded PEG4000 modified liposomes.

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“…Fluorescence spectroscopic studies Investigation of the interactions between quercetin and macromolecules. In particular, it was used in the formation of the dimeric assemblies of quercetin with cyclodextrins [91,[174][175][176][177][178][179][180].…”

Section: Molecular Dynamicsmentioning

confidence: 99%

Quercetin: A Potential Polydynamic Drug

Georgiou,

Kakava,

Routsi

et al. 2023

Molecules

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The study of natural products as potential drug leads has gained tremendous research interest. Quercetin is one of those natural products. It belongs to the family of flavonoids and, more specifically, flavonols. This review summarizes the beneficial pharmaceutical effects of quercetin, such as its anti-cancer, anti-inflammatory, and antimicrobial properties, which are some of the quercetin effects described in this review. Nevertheless, quercetin shows poor bioavailability and low solubility. For this reason, its encapsulation in macromolecules increases its bioavailability and therefore pharmaceutical efficiency. In this review, a brief description of the different forms of encapsulation of quercetin are described, and new ones are proposed. The beneficial effects of applying new pharmaceutical forms of nanotechnology are outlined.

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Optical Spectroscopy Study of the Interaction Between Quercetin and Human Serum Albumin

Cited by 8 publications

References 13 publications

Covalent Organic Frameworks: From Materials Design to Biomedical Application

Covalent Organic Frameworks: From Materials Design to Biomedical Application

PEG4000 modified liposomes enhance the solubility of quercetin and improve the liposome functionality: in vitro characterization and the cellular efficacy

Quercetin: A Potential Polydynamic Drug

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