Optimal dehydrothermal processing conditions to improve biocompatibility and durability of a weakly denatured collagen scaffold

Nakada, Akira; Shigeno, Keiji; Satô, Toshihiko; Hatayama, Takahide; Mariko, Wakatsuki; Nakamura, Tatsuo

doi:10.1002/jbm.b.33766

Cited by 14 publications

(8 citation statements)

References 33 publications

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“…Collagen is denatured by physical stress [167], temperature increase [168], or exposure to strong organic solvent [169]. Denatured collagen losses the native helical structure (triple helix) and subsequently forms a hydrolyzed product (gelatin) [169].…”

Section: Collagen Denaturation In Blending Strategymentioning

confidence: 99%

Collagen Scaffolds in Cartilage Tissue Engineering and Relevant Approaches for Future Development

Irawan

Sung

Higuchi

et al. 2018

Tissue Eng Regen Med

168

127

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BACKGROUND: Cartilage tissue engineering (CTE) aims to obtain a structure mimicking native cartilage tissue through the combination of relevant cells, three-dimensional scaffolds, and extraneous signals. Implantation of 'matured' constructs is thus expected to provide solution for treating large injury of articular cartilage. Type I collagen is widely used as scaffolds for CTE products undergoing clinical trial, owing to its ubiquitous biocompatibility and vast clinical approval. However, the long-term performance of pure type I collagen scaffolds would suffer from its limited chondrogenic capacity and inferior mechanical properties. This paper aims to provide insights necessary for advancing type I collagen scaffolds in the CTE applications. METHODS: Initially, the interactions of type I/II collagen with CTE-relevant cells [i.e., articular chondrocytes (ACs) and mesenchymal stem cells (MSCs)] are discussed. Next, the physical features and chemical composition of the scaffolds crucial to support chondrogenic activities of AC and MSC are highlighted. Attempts to optimize the collagen scaffolds by blending with natural/synthetic polymers are described. Hybrid strategy in which collagen and structural polymers are combined in non-blending manner is detailed. RESULTS: Type I collagen is sufficient to support cellular activities of ACs and MSCs; however it shows limited chondrogenic performance than type II collagen. Nonetheless, type I collagen is the clinically feasible option since type II collagen shows arthritogenic potency. Physical features of scaffolds such as internal structure, pore size, stiffness, etc. are shown to be crucial in influencing the differentiation fate and secreting extracellular matrixes from ACs and MSCs. Collagen can be blended with native or synthetic polymer to improve the mechanical and bioactivities of final composites. However, the versatility of blending strategy is limited due to denaturation of type I collagen at harsh processing condition. Hybrid strategy is successful in maximizing bioactivity of collagen scaffolds and mechanical robustness of structural polymer. CONCLUSION: Considering the previous improvements of physical and compositional properties of collagen scaffolds and recent manufacturing developments of structural polymer, it is concluded that hybrid strategy is a promising approach to advance further collagen-based scaffolds in CTE. Keywords Cartilage tissue engineering Á Type I collagen Á Articular chondrocytes Á Mesenchymal stem cells Á Hybrid scaffolds

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Section: Collagen Denaturation In Blending Strategymentioning

confidence: 99%

Collagen Scaffolds in Cartilage Tissue Engineering and Relevant Approaches for Future Development

Irawan

Sung

Higuchi

et al. 2018

Tissue Eng Regen Med

168

127

View full text Add to dashboard Cite

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“…Some crosslinkers like glutaraldehyde may also be cytotoxic and lead to calcification [ 18 , 23 ]. For physical crosslinking, dehydrothermal treatment (DHT) [ 24 , 25 ] and UV irradiation [ 10 , 26 , 27 ] are commonly used. However, these methods can lead to partial degradation of collagen and to protein denaturation [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Rose Bengal Crosslinking to Stabilize Collagen Sheets and Generate Modulated Collagen Laminates

Eckes

Braun

Wack

et al. 2020

IJMS

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For medical application, easily accessible biomaterials with tailored properties are desirable. Collagen type I represents a biomaterial of choice for regenerative medicine and tissue engineering. Here, we present a simple method to modify the properties of collagen and to generate collagen laminates. We selected three commercially available collagen sheets with different thicknesses and densities and examined the effect of rose bengal and green light collagen crosslinking (RGX) on properties such as microstructure, swelling degree, mechanical stability, cell compatibility and drug release. The highest impact of RGX was measured for Atelocollagen, for which the swelling degree was reduced from 630% (w/w) to 520% (w/w) and thickness measured under force application increased from 0.014 mm to 0.455 mm, indicating a significant increase in mechanical stability. Microstructural analysis revealed that the sponge-like structure was replaced by a fibrous structure. While the initial burst effect during vancomycin release was not influenced by crosslinking, RGX increased cell proliferation on sheets of Atelocollagen and on Collagen Solutions. We furthermore demonstrate that RGX can be used to covalently attach different sheets to create materials with combined properties, making the modification and combination of readily available sheets with RGX an attractive approach for clinical application.

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“…However, denaturation of collagen will occur when the temperature is over 150 °C. [122a] Ultraviolet irradiation will not influence the ligands and cell attachment at the highest crosslinking strengths 123. Unlike chemical crosslinking, no crosslinkers are incorporated into the matrix after physical crosslinking, which can reduce the potential cytotoxic effects caused by the crosslinker itself.…”

Section: Morphological Diversification Fabrication Methods and Potementioning

confidence: 99%

Advanced Collagen‐Based Biomaterials for Regenerative Biomedicine

Lin

Zhang

Macedo

et al. 2018

Adv Funct Materials

266

173

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In recent decades, collagen is one of the most versatile biomaterials used in biomedical applications, mostly due to its biomimetic and structural composition in the extracellular matrix (ECM). Several attempts are proposed for designing innovative collagen-based biomaterials and applying them in tissue regeneration. The regeneration of different tissues is prompted by different types and diverse physical forms of collagen-based biomaterials prepared by various methods. Based on such concepts, the source, structure, and classification of collagen are briefly introduced in this review. Here, the commonly used physical forms and modification methods of collagen-based biomaterials are reviewed, including hydrogels, scaffolds, and microspheres, followed by their applications in the regeneration of tissues and organs. Important proof-of-concept examples are described to demonstrate the outcomes on material characteristics, cellular reactions, and tissue regeneration. A concise assessment of the limitations that still exist and the developing trends in the future of collagen-based biomaterials are put forward.

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Optimal dehydrothermal processing conditions to improve biocompatibility and durability of a weakly denatured collagen scaffold

Cited by 14 publications

References 33 publications

Collagen Scaffolds in Cartilage Tissue Engineering and Relevant Approaches for Future Development

Collagen Scaffolds in Cartilage Tissue Engineering and Relevant Approaches for Future Development

Rose Bengal Crosslinking to Stabilize Collagen Sheets and Generate Modulated Collagen Laminates

Advanced Collagen‐Based Biomaterials for Regenerative Biomedicine

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