Optimal dietary L-glutamine level improves growth performance and intestinal histomorphometry of juvenile giant trahira (Hoplias lacerdae), a Neotropical carnivorous fish species

Ramos, Alfredo Rubén Palomino; Campelo, Daniel Abreu Vasconcelos; Carneiro, Cristiana Leonor da Silva; Zuanon, Jener Alexandre Sampaio; Matta, Sérgio Luís Pinto da; Furuya, Wilson Massamitu; Salaro, Ana Lúcia

doi:10.1016/j.aquaculture.2021.737469

Cited by 11 publications

(3 citation statements)

References 36 publications

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“…Glutamine is an essential functional amino acid that plays crucial regulatory roles in fish nutrition and physiology. Numerous studies have thoroughly documented the involvement of glutamine in growth regulation, appetite stimulation, protein synthesis, and skeletal muscle growth [52][53][54][55] . The application of Hilyses as a dietary feed supplement has been investigated in several previous studies utilizing a range of inclusion levels and feeding durations.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary fermented Saccharomyces cerevisiae extract (Hilyses) supplementation on growth, hematology, immunity, antioxidants, and intestinal health in Nile tilapia

Abd El-Naby,

El Asely,

Hussein

et al. 2024

Sci Rep

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This study investigated the effects of dietary supplementation with the product Hilyses on growth performance, feed utilization, nutrient composition, hematological parameters, serum biochemistry, immune function, antioxidant status, and digestive enzyme activity in juvenile Nile tilapia (Oreochromis niloticus, initial body weight 4.24 ± 0.01 g). The fish were fed diets supplemented with Hilyses at concentrations of 0, 1, 2, or 3 g/kg for a period of 8 weeks. The results showed that supplementation with Hilyses at levels up to 2 g/kg diet significantly improved final body weight, weight gain, specific growth rate, feed efficiency ratio, protein efficiency ratio, apparent protein utilization, and energy utilization compared to the control diet without Hilyses. Carcass crude protein content and moisture were significantly higher in Hilyses-fed groups, while crude lipid content decreased at the 3 g/kg supplementation level. Hilyses supplementation enhanced various hematological parameters, including increased red blood cell count, total leukocyte count, hemoglobin concentration, hematocrit, and mean corpuscular volume. Serum biochemistry and immune function markers like total protein, albumin, complement component C3, IgM, and IgG were significantly elevated in the 2 and 3 g/kg Hilyses groups. Antioxidant enzyme activities (catalase, glutathione peroxidase, total superoxide dismutase) were enhanced, and lipid peroxidation was reduced, in the 2 g/kg Hilyses group. Digestive enzyme activities, particularly protease and lipase, were also improved with Hilyses supplementation. Histological examination showed reduced lipid deposition in the liver and increased branching of intestinal villi at the 2 g/kg Hilyses level. Overall, these results indicated that dietary Hilyses supplementation at 2 g/kg diet optimizes growth, feed utilization, nutrient composition, hematology, immunity, antioxidant status, and digestive function in juvenile Nile tilapia.

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Section: Discussionmentioning

confidence: 99%

Effects of dietary fermented Saccharomyces cerevisiae extract (Hilyses) supplementation on growth, hematology, immunity, antioxidants, and intestinal health in Nile tilapia

Abd El-Naby,

El Asely,

Hussein

et al. 2024

Sci Rep

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“…In the brain, Gln is an important ammonia carrier and plays an important role in removing ammonia toxicity from the brain [28]. In the intestine, Gln plays an important role in maintaining and repairing the structural and functional integrity of the intestine and normal immune response [29]. In the liver, Gln promotes the production of glutathione and enhances the antioxidant capacity [30].…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Nutrition Regulation of the Glutamine Synthetase Gene in Triploid Crucian Carp

Zhang

Zhao

Chen

et al. 2022

Fishes

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Glutamine synthetase (GS) is a key enzyme that catalyzes the synthesis of glutamine from glutamate, which plays a role in the promotion of muscle cell growth and in improving the flavor of meats. In this study, a GS gene encoding 371 amino acids was cloned from triploid crucian carp and showed the highest level of similarity with the GS gene found in Cyprinus carpio. Meanwhile, GS was differentially expressed in different tissues, and its day–night expression changes showed obvious oscillation. Additionally, the effects of glutamate and glutamine on GS expression in muscle cells were investigated in vitro and in vivo. We found that its expression was obviously increased due to high levels of glutamate (2 mg/mL) but decreased by glutamine in vitro. However, it was significantly promoted by glutamate and glutamine in vivo, with an optimal concentration of 2%. Furthermore, the use of lysine–glutamate dipeptides as feed additives also had a positive influence on GS expression (the optimal concentration is 0.8%). Finally, we explored the effects of different protein levels and sources on the expression of GS, and the results demonstrated that GS had the highest expression at the 35% protein level, but no significant differences were observed in the different protein sources between the fish meal diet (FM) and the mixed diet comprising soybean meal and rapeseed meal (SM). This study sheds new light on the regulation of GS in teleost fish and provides new perceptions and strategies for the formulation of high-quality feed for triploid crucian carp.

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“…Arginine is an α amino acid that is mainly used in protein biosynthesis and promotes the growth and immunity of aquatic organisms (Hoseini et al, 2019). Glutamine is a non‐essential amino acid that has unique and complex physiological functions, such as promoting animal growth, enhancing immunity and maintaining a redox state (Palomino Ramos et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Comparative proteome analysis reveals possible heterosis for growth, immunity and antioxidation mechanisms in Macrobrachium nipponense hybrid offspring and parent populations

Jiang

et al. 2022

Aquaculture Research

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Macrobrachium nipponense, also known as river prawn, belongs to the phylum Arthropoda, Crustacea, Decapoda, and is widely distributed in freshwater and low-salinity estuaries in Japan, Korea, Vietnam and China (Li et al., 2019). M. nipponense is an important economic species in China, which is favoured by aquaculture farmers due to their short reproduction cycle, delicious meat and rich nutritional value (Hongtuo et al., 2012). In recent years, with the gradual expansion of artificial breeding operations, issues in the culturing of M. nipponense have gradually emerged, including the degradation of shrimp germplasm resources and the decline of production, which has restricted the further development of the M. nipponense culture industry (Hongtuo et al., 2012). The deterioration of M. nipponense germplasms has caused many problems, including sexual precocity, which has resulted in the failure of individual M. nipponense to grow to the size required by commercial specifications, which has greatly affected its sales market (Bai et al., 2015). Therefore, it is necessary

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Optimal dietary L-glutamine level improves growth performance and intestinal histomorphometry of juvenile giant trahira (Hoplias lacerdae), a Neotropical carnivorous fish species

Cited by 11 publications

References 36 publications

Effects of dietary fermented Saccharomyces cerevisiae extract (Hilyses) supplementation on growth, hematology, immunity, antioxidants, and intestinal health in Nile tilapia

Effects of dietary fermented Saccharomyces cerevisiae extract (Hilyses) supplementation on growth, hematology, immunity, antioxidants, and intestinal health in Nile tilapia

Molecular Characterization and Nutrition Regulation of the Glutamine Synthetase Gene in Triploid Crucian Carp

Comparative proteome analysis reveals possible heterosis for growth, immunity and antioxidation mechanisms in Macrobrachium nipponense hybrid offspring and parent populations

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