Optimal Lymph Node Harvest in Rectal Cancer (UICC Stages II and III) after Preoperative 5-FU–based Radiochemotherapy. Acetone Compression is a New and Highly Efficient Method

Abstract

The AC method is the method of choice for accurate LN staging in locally advanced rectal cancer, especially after preoperative RCT, and is well suited for routine gastrointestinal pathology workup.

Methylene blue-assisted lymph node dissection technique is not associated with an increased detection of lymph node metastases in colorectal cancer

“…For decades, fat clearance techniques have been the only approach to improve lymph node harvest in colorectal cancers. [18][19][20][21][22] We recently introduced the methylene blue-assisted lymph node dissection technique as a simple, cheap and very effective alternative method. Moreover, the usefulness of this technique has been approved by several other groups.…”

“…17 Fat clearance is a well-known technique to improve lymph node harvest not only in colorectal cancer. [18][19][20][21][22] However, all of these techniques are time consuming, expensive and need potentially toxic agents. In 2007, we introduced the methylene blue-assisted lymph node dissection method as a cheap and simple alternative method to improve lymph node harvest in gastrointestinal cancers.…”

Luteolin Induced-growth Inhibition and Apoptosis of Human Esophageal Squamous Carcinoma Cell Line Eca109 Cells in vitro

“…It's necessary to discover efficient pathway to impede the ectopic proliferation and accelerate death of tumor cells. Although, broad-spectrum anticancer drug such as 5-FU (a pyrimidine analogue) is widely used for clinical therapy (Gehoff et al, 2012;Pera et al, 2012;Su, 2012), the large side effects which it has been shown are a still limit aspect for use. Luteolin, a natural dietary flavonoid isolated from various plants has the potential role to slow or prevent the growth of tumor cell lines (Ko et al, 2002;Ju et al, 2007;Lim, et al, 2007;Chiu et al, 2008).…”

Comprehensive lymph node morphometry in rectal cancer using acetone compression

“…Cases with WME were prepared in a second pathological institution (Department of Pathology, University Medical Center Göttingen) as part of a study of LN retrieval after CRT in the context of the CAO/ARO/AIO-04 phase III trial 8 9 12. WME has been described previously 8…”

“…AC was performed as described in the literature 10 12. In brief, the tissue in the tumour region was cut in cross sections and checked for visible or palpable LN, that is, manual dissection of the tumour region was performed.…”