Optimal method of gold nanoparticle administration in melanoma‑bearing mice

Komatsu, Tetsuya; Nakamura, Katsumasa; Okumura, Yasuhiro; Konishi, Kenta

doi:10.3892/etm.2018.5746

Cited by 5 publications

(3 citation statements)

References 22 publications

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“…This is in line with the recent data showing that 1 mg/kg AuNPs might be around the real therapeutical dose. 25 , 36 , 37 BSA-AuNPs are considered a safe platform among various AuNPs types, nevertheless, their biocompatibility still needs to be carefully assessed. In one of the recent publications, five different doses of AuNPs coated with BSA ranging from 1.16 mg/kg to 5.84 mg/kg were tested for 8 consecutive days after intravenous injections in mice.…”

Section: Discussionmentioning

confidence: 99%

Long-Term Accumulation, Biological Effects and Toxicity of BSA-Coated Gold Nanoparticles in the Mouse Liver, Spleen, and Kidneys

Jakic,

Selc,

Razga

et al. 2024

IJN

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Introduction Gold nanoparticles are promising candidates as vehicles for drug delivery systems and could be developed into effective anticancer treatments. However, concerns about their safety need to be identified, addressed, and satisfactorily answered. Although gold nanoparticles are considered biocompatible and nontoxic, most of the toxicology evidence originates from in vitro studies, which may not reflect the responses in complex living organisms. Methods We used an animal model to study the long-term effects of 20 nm spherical AuNPs coated with bovine serum albumin. Mice received a 1 mg/kg single intravenous dose of nanoparticles, and the biodistribution and accumulation, as well as the organ changes caused by the nanoparticles, were characterized in the liver, spleen, and kidneys during 120 days. Results The amount of nanoparticles in the organs remained high at 120 days compared with day 1, showing a 39% reduction in the liver, a 53% increase in the spleen, and a 150% increase in the kidneys. The biological effects of chronic nanoparticle exposure were associated with early inflammatory and fibrotic responses in the organs and were more pronounced in the kidneys, despite a negligible amount of nanoparticles found in renal tissues. Conclusion Our data suggest, that although AuNPs belong to the safest nanomaterial platforms nowadays, due to their slow tissue elimination leading to long-term accumulation in the biological systems, they may induce toxic responses in the vital organs, and so understanding of their long-term biological impact is important to consider their potential therapeutic applications.

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Section: Discussionmentioning

confidence: 99%

Long-Term Accumulation, Biological Effects and Toxicity of BSA-Coated Gold Nanoparticles in the Mouse Liver, Spleen, and Kidneys

Jakic,

Selc,

Razga

et al. 2024

IJN

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“…This finding suggests a synergistic effect between the nanoparticles and EBRT. Based on these results, we suggest that a combination of nanoparticles and EBRT is appropriate for inhibiting the growth of melanoma tumours [ 13 , 14 , 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Radiosensitising effect of iron oxide‐gold nanocomplex for electron beam therapy of melanoma in vivo by magnetic targeting

Mohamadkazem

Neshasteh-Riz

Amini

et al. 2023

IET Nanobiotechnology

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Melanoma is a dangerous type of skin cancer sometimes treated with radiotherapy. However, it induces damage to the surrounding healthy tissue and possibly further away areas. Therefore, it is necessary to give a lower dose to the patient with targeted therapy. In this study, the radio‐sensitising effect of gold‐coated iron oxide nanoparticles on electron beam radiotherapy of a melanoma tumour with magnetic targeting in a mouse model was investigated. Gold‐coated iron oxide nanoparticles were prepared in a steady procedure. The melanoma tumour model was induced in mice. Animals were divided into five groups: (1) normal; (2) melanoma; (3) gold‐coated iron oxide nanoparticles alone; (4) electron beam radiotherapy; (5) electron beam radiotherapy plus gold‐coated iron oxide nanoparticles. The magnet was placed on the tumour site for 2 h. The tumours were then exposed to 6 MeV electron beam radiotherapy for a dose of 8 Gy. Inductively coupled plasma optical emission spectrometry test, hematoxylin and eosin staining, and enzyme‐linked immunosorbent assay blood test were also performed. Gold‐coated iron oxide nanoparticles with magnetic targeting before electron beam radiotherapy reduced the growth of the tumour compared to the control group. Blood tests did not show any significant toxicity. Deposition of nanoparticles was more in the tumour and spleen tissue and to a lesser extent in the liver, kidney, and lung tissues. The synergistic effect of nanoparticles administered by the intraperitoneal route and then concentrated into the tumour area by application of an external permanent magnet, before delivery of the electron beam radiotherapy improved the overall cancer treatment outcome and prevented metal distribution side effects.

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“… 50 Anijdan et al found that tumor growth was partially inhibited, albeit without a statistically significant survival benefit, following intratumoral injection of AuNPs (~500–1000 μg) under X-ray irradiation at 20 Gy. 51 The study by Komatsu et al 52 demonstrated regardless of intravenous administration or intratumoral injection of AuNPs (400 μg), X-ray irradiation at 10 Gy showed no tumor inhibition or statistical survival benefit. In our study, intratumoral injection of mAuNPs (280 μg) combined with carbon ion irradiation at 4 Gy contributed to a significant radiation enhancement of 94% in tumor growth inhibition and a significant 75% improvement in survival time.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Efficacy of Carbon Ion Irradiation Enhanced by 11-MUA-Capped Gold Nanoparticles: An in vitro and in vivo Study

Zhang¹,

Yu²,

Jin³

et al. 2021

IJN

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Purpose: Gold nanoparticles (AuNPs) are widely studied as radiosensitizers, but their radiosensitization in carbon ion radiotherapy is unsatisfactory. There is a lack of in vivo data on the radiosensitization of AuNPs under carbon ion irradiation. This study focused on the radiosensitization effect of AuNPs in the mouse melanoma cell line B16-F10 in vitro and in vivo. Materials and Methods: 11-mercaptoundecanoic acid (11-MUA)-coated gold (Au) nanoparticles (mAuNPs) formulations were prepared and characterized. To verify the radiosensitization effect of mAuNPs, hydroxyl radicals were generated in aqueous solution, and the detection of intracellular reactive oxygen species (ROS) and clone survival were carried out in vitro. The tumor growth rate (TGR) and survival of mice were analyzed to verify the radiosensitization effect of mAuNPs in vivo. The apoptosis of tumor cells was detected, and the expression of key proteins in the apoptosis pathway was verified by immunohistochemistry. Results: The intracellular ROS level in B16-F10 cells was enhanced by mAuNPs under carbon ion irradiation. The sensitization rate of mAuNPs was 1.22 with a 10% cell survival rate. Compared with irradiation alone, the inhibitory effect of mAuNPs combined with carbon ion irradiation on tumor growth was 1.94-fold higher, the survival time of mice was prolonged by 1.75-fold, and the number of apoptotic cells was increased by 1.43-fold. The ratio of key proteins Bax and Bcl2 in the apoptosis pathway was up-regulated, and the expression of caspase-3, a key executor of the apoptosis pathway, was up-regulated. Conclusion:In in vivo and in vitro experiments, mAuNPs showed radiosensitivity to carbon ion irradiation. The sensitization effect of mAuNPs on mice tumor may be achieved by activating the mitochondrial apoptosis pathway and increasing tumor tissue apoptosis. To our best knowledge, the present study is the first in vivo evidence for radiosensitization of mAuNPs in tumor-bearing mice exposed to carbon ion irradiation.

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Optimal method of gold nanoparticle administration in melanoma‑bearing mice

Cited by 5 publications

References 22 publications

Long-Term Accumulation, Biological Effects and Toxicity of BSA-Coated Gold Nanoparticles in the Mouse Liver, Spleen, and Kidneys

Long-Term Accumulation, Biological Effects and Toxicity of BSA-Coated Gold Nanoparticles in the Mouse Liver, Spleen, and Kidneys

Radiosensitising effect of iron oxide‐gold nanocomplex for electron beam therapy of melanoma in vivo by magnetic targeting

Therapeutic Efficacy of Carbon Ion Irradiation Enhanced by 11-MUA-Capped Gold Nanoparticles: An in vitro and in vivo Study

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