Optimal seed water content and storage temperature for preservation of Populus nigra L. germplasm

Suszka, Jan; Plitta, Beata P.; Michalak, Marcin; Bujarska-Borkowska, Barbara; Tylkowski, T.; Chmielarz, Paweł

doi:10.1007/s13595-014-0368-2

Cited by 22 publications

(31 citation statements)

References 12 publications

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“…Seeds were stored in closed plastic vials at four different temperatures (3°C, −3°C, −10°C, −20°C) or cryostored at −196°C in liquid nitrogen (LN) for 24 months. Chosen temperatures are recommended for shortterm (3°C, −3°C) (Tylkowski 2010; Suszka et al 2014) or long-term (−10°C, −20°C, −196°C) (Sato 1955;Stanton and Villar 1996; Tylkowski 2010) storage for poplar seeds. Additionally, fresh seeds that were collected in 2012 were used as a control.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Additionally, fresh seeds that were collected in 2012 were used as a control. Details of the storage protocols were described in Suszka et al (2014) and Michalak et al (2014).…”

Section: Plant Materialsmentioning

confidence: 99%

“…Various seed storage temperatures have been reported for P. nigra, which have resulted in the ability to store the seeds for different lengths of time and still retain sufficient levels of seed viability (Stanton and Villar 1996;Tylkowski 2010;Michalak et al 2014;Suszka et al 2014). Thus far in the literature, optimal seed storage conditions have been assessed on the basis of seed germination (Popova et al 2012;Malik et al 2013;Suszka et al 2014) or seedling emergence (Michalak et al 2013;Popova et al 2013). These parameters do measure the impact of storage temperature on seed viability and initial emergence.…”

Section: Introductionmentioning

confidence: 99%

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Effect of seed storage temperature on fine root development and mycorrhizal colonization of young Populus nigra seedlings

Mucha

Szymańska

Zadworny

et al. 2015

Annals of Forest Science

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Key message Seed storage temperature influences root anatomy of the endangered Populus nigra, and consequently may alter nutrient absorption. A lower temperature during seed storage (−20 and −196 °C) may preserve the potential for a suitable root system development after germination. Context Seed storage conditions can be an important determinant of later seedling growth of Populus nigra L., an endangered tree species. Aims We tested whether long-term seed storage temperature, −10, −20 or −196 °C, affects the pattern of seedling root traits responsible for resource acquisition as compared to seedlings of fresh seeds. Methods We analysed the morphology, anatomy, degree of mycorrhizal colonization, and biochemical composition of roots developed from seed stored for 24 months at five different temperatures (from 3 to −196 °C) commonly used to preserve genetic resources. Results Except for root anatomy, we found no relationship between seed storage temperature and the root traits of seedlings. Among the various storage conditions, the proportion of roots with primary development in the first four orders was similar in seedlings developed from fresh seeds of from seeds stored at −196 or −20 °C. Nitrogen content in the roots was positively correlated with the proportion of (i) roots with primary development and (ii) the cortex width in the root diameter. Conclusions Higher temperatures during seed storage reduced the proportion of roots with absorptive function (with primary development). Therefore, for preservation of P. nigra seeds we recommend lower temperatures such as −20 and −196 °C.

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Section: Plant Materialsmentioning

confidence: 99%

“…Additionally, fresh seeds that were collected in 2012 were used as a control. Details of the storage protocols were described in Suszka et al (2014) and Michalak et al (2014).…”

Section: Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of seed storage temperature on fine root development and mycorrhizal colonization of young Populus nigra seedlings

Mucha

Szymańska

Zadworny

et al. 2015

Annals of Forest Science

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“…Survival and regeneration was better noticed either on the embryonic axes that were chopped off and dehydrated prior cryopreservation treatment or from the embryonic axes extracted from already cryopreserved seeds. Suszka et al (2014) reported that both fresh and desiccated seeds of Populus nigra could be preserved using standard cryopreservation techniques for about two years.…”

Section: Influence Of Moisture Contentmentioning

confidence: 99%

“…Gantait et al (2015), on the other hand, unloaded the sample in 1.2 M sucrose after quick thawing and then relocated onto double layered sterile filter paper kept on Petri dishes enclosing semi-solid MS medium with 3% (w/v) sucrose and 100 mg l -1 myoinositol devoid of any plant growth regulators. The samples were given dark phase for consecutive 7 days before disclosure to light with a 16 h photoperiod at 26±2 o C. Suszka et al (2014) germinated the desiccated seeds in a Jacobsen germinator. Moist filter paper was used to rehydrate the seeds and cool white light was supplied on a 12 h cycle.…”

Section: Post-cryopreservation Regrowthmentioning

confidence: 99%

Cryopreservation of Forest Tree Seeds: A Mini-Review

Gantait¹,

Kundu²,

Wani³

et al. 2016

Journal of Forest and Environmental Science

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Since forest trees form the basis of forest ecosystem, their prolong subsistence is crucial for various flora and fauna. The foremost challenges to sustain the forest ecosystem comprise of the declining forest tree population accompanied with structural changes due to afforestation and exploitation of forest area, environment changes, pests, pollution, and introgressive hybridization. For ex situ conservation approach, in vitro techniques encompass basic role for conserving tree genetic resources, predominantly where natural propagules like recalcitrant seed might not be appropriate for long-term conservation. The practice includes restricted growth techniques, conventional micropropagation, production and storage of synthetic seeds, and cryopreservation. Even though these practices have been applied chiefly to herbaceous species, but recently, woody species were also focused upon. Key conceptions, challenges and techniques for forest tree seed conservation are discussed briefly in this review with special emphasis on some successful cryopreservation approaches for long-term storage.

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Genetic Resources

Gore

Gowthmi

Tripathi

et al. 2021

Environment and Climate-Smart Food Production

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Optimal seed water content and storage temperature for preservation of Populus nigra L. germplasm

Abstract: ) is an alluvial forest tree species whose genetic pool is decreasing in Europe. Poplar trees produce short-lived seeds that do not store well.

Cited by 22 publications

References 12 publications

Effect of seed storage temperature on fine root development and mycorrhizal colonization of young Populus nigra seedlings

Effect of seed storage temperature on fine root development and mycorrhizal colonization of young Populus nigra seedlings

Cryopreservation of Forest Tree Seeds: A Mini-Review

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