The beggartick (Bidens subalternans L.) is one of the main weeds present in agricultural crops, capable of adapting to different environmental conditions. The water stress caused by water deficiency can affect the germination of weed seeds and, consequently, their capacity to colonize the agroecosystem. Knowledge of germination under water stress can be important to obtain an adequate management of the species in the agricultural systems. The objective of this study was to determine the effects of water stress on the germination process and vigor of two batches of Bidens subalternans seeds under different osmotic agents. It was completely randomized experimental design was used, with four replicates of 25 seeds. The treatments were arranged in factorial 6 × 2, with the first factor corresponding six levels of osmotic potentials (0.0, -0.2, -0.4, -0.6, -0.8 and -1.0 MPa) and the second the batches (Pernambuco and Ceará). For the simulation of the water stress, the test of germination was installed in substrate paper blotting, moistened with solutions of polyethylene glycol (PEG 6000) and mannitol. The analyzed variables were germination, germination speed index, length and seedling dry mass. Water stress reduced germination, seed vigor and growth of seedlings in all batches of B. subalternans. Seeds of B. subalternans presented greater tolerance to stress induced by mannitol than to PEG-6000 in terms of germination and germination speed index. Regardless of the osmotic agent used for stress induction, B. subalternans seeds did not tolerate water stress higher than -0.4 MPa.