Optimisation and validation of a sensitive high-performance liquid chromatography assay for routine measurement of pyridoxal 5-phosphate in human plasma and red cells using pre-column semicarbazide derivatisation

Talwar, Dinesh; Quasim, Tara; McMillan, Donald C.; Kinsella, John; Williamson, Cathy; O’Reilly, Denis St. J.

doi:10.1016/s1570-0232(03)00320-9

Cited by 108 publications

(89 citation statements)

References 22 publications

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“…These concentrations are similar to those reported by others (9,22,27,40 ). We also analyzed samples from patients with cardiovascular disease who received vitamin B 6 (40 mg/day PN; n ϭ 30) or placebo (n ϭ 30) for 1 month and observed that PLP, PL, and PA concentrations were 10-to 100-fold higher in vitamin B 6 -supplemented patients than in the placebo group (Table 4).…”

Section: Performance Of the Methodssupporting

confidence: 92%

“…LOD varied from 0.1 nmol/L for PN to 4 nmol/L for PMP and FAD. The LOD values obtained were comparable to those of other B 6 (20,25,27,29 ) and B 2 (39 ) assays.…”

Section: Performance Of the Methodssupporting

confidence: 79%

“…For published B 6 assays, there are often no data on assay imprecision, or the imprecision reported varies widely, from 0.6%-5.6% (29 ), 2%-6% (9,20 ), and 4%-12% (27 ), to 7%-67% (18 ).…”

Section: Performance Of the Methodsmentioning

confidence: 99%

“…Vitamin B 6 methods are usually based on HPLC with fluorescence detection, which requires derivatization of PLP and complete chromatographic separation of the analytes. This often gives relatively long analysis times of ϳ30 min or longer (18,19,23,(25)(26)(27). In studies involving vitamin B 6 in plasma, usually only PLP was determined (20, 30 -34 ), but in some cases (5,10,29,35,36 ), several B 6 vitamers were measured.…”

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confidence: 99%

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Multianalyte Quantification of Vitamin B6 and B2 Species in the Nanomolar Range in Human Plasma by Liquid Chromatography–Tandem Mass Spectrometry

Midttun¹,

Hustad²,

Solheim³

et al. 2005

Clinical Chemistry

104

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Section: Performance Of the Methodssupporting

confidence: 92%

“…LOD varied from 0.1 nmol/L for PN to 4 nmol/L for PMP and FAD. The LOD values obtained were comparable to those of other B 6 (20,25,27,29 ) and B 2 (39 ) assays.…”

Section: Performance Of the Methodssupporting

confidence: 79%

“…For published B 6 assays, there are often no data on assay imprecision, or the imprecision reported varies widely, from 0.6%-5.6% (29 ), 2%-6% (9,20 ), and 4%-12% (27 ), to 7%-67% (18 ).…”

Section: Performance Of the Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Multianalyte Quantification of Vitamin B6 and B2 Species in the Nanomolar Range in Human Plasma by Liquid Chromatography–Tandem Mass Spectrometry

Midttun¹,

Hustad²,

Solheim³

et al. 2005

Clinical Chemistry

104

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“…Plasma PLP, PL, and 4-PA were determined by highperformance liquid chromatography as previously described (Talwar et al, 2003b). The fluorescence detector's excitation and emission wavelengths were 320 and 420 nm, respectively.…”

Section: Biochemical Measurementsmentioning

confidence: 99%

Vitamin B6 supplementation increases immune responses in critically ill patients

Cheng

Chang²,

Lee

et al. 2006

Eur J Clin Nutr

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Expression Changes in Epigenetic Gene Pathways Associated With One‐Carbon Nutritional Metabolites in Maternal Blood From Pregnancies Resulting in Autism and Non‐Typical Neurodevelopment

et al. 2020

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The prenatal period is a critical window for the development of autism spectrum disorder (ASD). The relationship between prenatal nutrients and gestational gene expression in mothers of children later diagnosed with ASD or non‐typical development (Non‐TD) is poorly understood. Maternal blood collected prospectively during pregnancy provides insights into the effects of nutrition, particularly one‐carbon metabolites, on gene pathways and neurodevelopment. Genome‐wide transcriptomes were measured with microarrays in 300 maternal blood samples in Markers of Autism Risk in Babies‐Learning Early Signs. Sixteen different one‐carbon metabolites, including folic acid, betaine, 5′‐methyltretrahydrofolate (5‐MeTHF), and dimethylglycine (DMG) were measured. Differential expression analysis and weighted gene correlation network analysis (WGCNA) were used to compare gene expression between children later diagnosed as typical development (TD), Non‐TD and ASD, and to one‐carbon metabolites. Using differential gene expression analysis, six transcripts (TGR‐AS1, SQSTM1, HLA‐C, and RFESD) were associated with child outcomes (ASD, Non‐TD, and TD) with genome‐wide significance. Genes nominally differentially expressed between ASD and TD significantly overlapped with seven high confidence ASD genes. WGCNA identified co‐expressed gene modules significantly correlated with 5‐MeTHF, folic acid, DMG, and betaine. A module enriched in DNA methylation functions showed a suggestive protective association with folic acid/5‐MeTHF concentrations and ASD risk. Maternal plasma betaine and DMG concentrations were associated with a block of co‐expressed genes enriched for adaptive immune, histone modification, and RNA processing functions. These results suggest that the prenatal maternal blood transcriptome is a sensitive indicator of gestational one‐carbon metabolite status and changes relevant to children's later neurodevelopmental outcomes. Lay Summary Pregnancy is a time when maternal nutrition could interact with genetic risk for autism spectrum disorder. Blood samples collected during pregnancy from mothers who had a prior child with autism were examined for gene expression and nutrient metabolites, then compared to the diagnosis of the child at age three. Expression differences in gene pathways related to the immune system and gene regulation were observed for pregnancies of children with autism and non‐typical neurodevelopment and were associated with maternal nutrients.

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Optimisation and validation of a sensitive high-performance liquid chromatography assay for routine measurement of pyridoxal 5-phosphate in human plasma and red cells using pre-column semicarbazide derivatisation

Cited by 108 publications

References 22 publications

Multianalyte Quantification of Vitamin B6 and B2 Species in the Nanomolar Range in Human Plasma by Liquid Chromatography–Tandem Mass Spectrometry

Multianalyte Quantification of Vitamin B6 and B2 Species in the Nanomolar Range in Human Plasma by Liquid Chromatography–Tandem Mass Spectrometry

Vitamin B6 supplementation increases immune responses in critically ill patients

Expression Changes in Epigenetic Gene Pathways Associated With One‐Carbon Nutritional Metabolites in Maternal Blood From Pregnancies Resulting in Autism and Non‐Typical Neurodevelopment

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