Optimisation of an HPLC method for the simultaneous quantification of the major sugars and organic acids in grapevine berries

Eyéghè-Bickong, Hans A.; Alexandersson, Erik O.; Gouws, Liezel M.; Young, Philip R.; Vivier, Melané A.

doi:10.1016/j.jchromb.2011.12.011

Cited by 112 publications

(70 citation statements)

References 22 publications

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“…Determination of organic acid profile of umezu was conducted by high performance liquid chromatography (HPLC) as previously described [9]. An Agilent 1200 series HPLC system (Agilent Technologies, Palo Alto, California, USA) equipped with VWD detector was used to analyze organic acids.…”

Section: Analysis Of Organic Acids By Hplcmentioning

confidence: 99%

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Chemical Composition and Antioxidant Activities of Five Samples of Prunus mume Umezu from Different Factories in South and East China

Chen

Lin

Liu

et al. 2017

Journal of Food Quality

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This paper investigated chemical composition and antioxidant activities of umezu, pickling liquid of Prunus mume, from different factories in South and East China. The organic acid and phenolic acid profiles were also analyzed. Results showed that umezu was rich in organic acids and extremely sour as P. mume fruit in addition to its high NaCl level (≥20%). Total acid in umezu was more than 43.78 g/L in which main organic acids were citric acid and malic acid. Umezu contained more than 250.54 mg GAE/L total phenolic in which dominant phenolic acids were hydroxycinnamic acid derivatives. Umezu exhibited powerful antioxidant activities in ORAC, ABTS, DPPH, and FRAP assays. Reducing sugar, phenolic compounds, and antioxidant activities of umezu were affected by sample origins and fruit cultivars. Given its rich flavor components and high antioxidant activity, umezu could serve as a new dietary supplement or a natural preservative in food industry.

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Section: Analysis Of Organic Acids By Hplcmentioning

confidence: 99%

“…The characteristic sourness of P. mume fruits is caused by the presence of free organic acids in this fruit [9,18]. Generally, the total titratable acid of P. mume fruits could be as high as 6.0-7.0% on a fresh weight basis, though its profile is diversified [2].…”

Section: Organic Acid Profilementioning

confidence: 99%

Chemical Composition and Antioxidant Activities of Five Samples of Prunus mume Umezu from Different Factories in South and East China

Chen

Lin

Liu

et al. 2017

Journal of Food Quality

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“…Typically the calibration curve generated using an ELSD is sigmoidal or exponential including fructose, sorbitol, glucose and sucrose which produced second order polynomial curves. Further, to convert the calibration curves from polynomial to linear, the log 10 of the peaks area and calibration concentrations were calculated [8]. According to the chromatographic condition in table 1, the mixed standard solution was measured and the linear equation was constructed by the quality logarithm of fructose, sorbitol, glucose and sucrose versus peak area logarithm.…”

Section: The Optimization Of Mobile Phasementioning

confidence: 99%

Evaluation of Sugar Content and Composition in ‘Angeleno’ Plum Fruit by HPLC-ELSD

Zhao¹,

Li²,

Wang³

et al. 2017

dteees

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Abstract. In order to evaluate the sugar content and composition of 'Angeleno' plum, a method for the simultaneous determination of fructose, glucose, sucrose, sorbitol in Angeleno fruit was developed with HPLC-ELSD .The analysis was performed on an Agilent Zorbax column with a gradient program, acetonitrile-water as the mobile phase . The column temperature was 35 ℃ and flow rate was 1.40 ml/min, evaporative light scattering detector, the drift tube temperature was 80 ℃. The Results showed that 'Angeleno' plum fruit mainly contain fructose, sorbitol, glucose and sucrose , four kinds of sweet substances, the average recoveries in the 92.08% ~101.31%; RSD in the range of 0.52% ~ 3.68%. In a conclusion, the method was accuracy, sensitivity and feasibility to analyze the four kinds of soluble sugar in Angeleno plum fruit.

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“…In this type of chromatography, the analytical conditions allow that part of the mobile phase to remain immobilized in the stationary phase, and the separation is due to the differences in the partitioning of the molecular solute between the solvent from the mobile phase and the solvent immobilized on the resin [43]. However, no analytical method has been described in the literature for the determination of urinary tt-MA as a BEI for benzene using HPLC with an Aminex HPX-87H column, although this analyte and other volatile organic acids have been determined in various other sample matrices using this type of column [44][45][46].…”

Section: Introductionmentioning

confidence: 99%

Method for the Determination of Benzene Metabolite t,t-Muconic Acid in Urine by HPLC-UV with an Ion Exclusion Column

et al. 2016

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Abstract:The benzene metabolite trans,trans-muconic acid (tt-MA) is widely used as a biological indicator of exposure to this xenobiotic. An analytical method was developed and validated for the determination of urinary tt-MA using solid phase extraction onto a Strata SAX cartridge. The analysis was performed by HPLC with an Aminex HPX-87H ion exclusion column and a UV diode array detector. The sample preparation conditions were optimized using a 2 4´1 factorial design.Comparison of the slopes of standard analytical curves prepared in aqueous solution and urine showed that the biological matrix suppressed the tt-MA signal by around 50%, so the analytical curves were prepared with tt-MA standard at low concentrations in pooled urine. The analytical curves in the range of 5-500 µg¨L´1 showed determination coefficients values (R 2 ) > 0.99 for tt-MA standard in water and R 2 > 0.98 for tt-MA standards in pooled urine. The coefficients of variation obtained using seven replicates were lower than 3.6%, and recoveries of tt-MA from solutions containing 5, 25, 50 and 100 µg¨L´1 of the analyte were in the range 85%-90%, demonstrating the satisfactory precision and accuracy of the method. The limits of detection and quantification were 0.11 and 0.36 µg¨L´1, respectively. The benefits of this new method developed is the possibility of complete chromatographic peak separation for the determination of tt-MA at baseline, without matrix components' interference as normally found in the C18 column. This is the first time that this chromatographic column has been used for the analysis of tt-MA in urine.

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Optimisation of an HPLC method for the simultaneous quantification of the major sugars and organic acids in grapevine berries

Cited by 112 publications

References 22 publications

Chemical Composition and Antioxidant Activities of Five Samples of Prunus mume Umezu from Different Factories in South and East China

Chemical Composition and Antioxidant Activities of Five Samples of Prunus mume Umezu from Different Factories in South and East China

Evaluation of Sugar Content and Composition in ‘Angeleno’ Plum Fruit by HPLC-ELSD

Method for the Determination of Benzene Metabolite t,t-Muconic Acid in Urine by HPLC-UV with an Ion Exclusion Column

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