Optimisation of biodegradation conditions for cyanide removal by Serratia marcescens strain AQ07 using one-factor-at-a-time technique and response surface methodology

Karamba, Kabiru Ibrahim; Ahmad, Siti Aqlima; Zulkharnain, Azham; Syed, Mohd Arif; Khalil, Khalilah Abdul; Shamaan, Nor Aripin; Dahalan, Farrah Aini; Shukor, Mohd Yunus

doi:10.1007/s12210-016-0516-8

Cited by 45 publications

(36 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Optimization of the conditions for diesel degradation by microorganisms is essential in order to ensure successful treatment of diesel. Optimization means establishing the foremost solution, representing the best compromise amidst several varying demands subject to predefined requirements called restrictions (Karamba et al 2016). Optimization is very significant in biotechnological processes.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica

et al. 2020

Self Cite

View full text Add to dashboard Cite

Bioremediation has been proposed as a means of dealing with oil spills on the continent. However, the introduction of non-native organisms, including microbes, even for this purpose would appear to breach the terms of the Environmental Protocol to the Antarctic Treaty. This study therefore aimed to optimize the growth conditions and diesel degradation activity of the Antarctic native bacteria Arthrobacter spp. strains AQ5-05 and AQ5-06 through the application of a one-factor-at-a-time (OFAT) approach. Both strains were psychrotolerant, with the optimum temperature supporting diesel degradation being 10–15°C. Both strains were also screened for biosurfactant production and biofilm formation. Their diesel degradation potential was assessed using Bushnell–Haas medium supplemented with 0.5% (v/v) diesel as the sole carbon source and determined using both gravimetric and gas chromatography and mass spectrophotometry analysis. Strain AQ5-06 achieved 37.5% diesel degradation, while strain AQ5-05 achieved 34.5% diesel degradation. Both strains produced biosurfactants and showed high biofilm adherence. Strains AQ5-05 and AQ5-06 showed high cellular hydrophobicity rates of 73.0% and 81.5%, respectively, in hexadecane, with somewhat lower values of 60.5% and 70.5%, respectively, in tetrahexadecane. Optimized conditions identified via OFAT increased diesel degradation to 41.0% and 47.5% for strains AQ5-05 and AQ5-06, respectively. Both strains also demonstrated the ability to degrade diesel in the presence of heavy metal co-pollutants. This study therefore confirms the potential use of these cold-tolerant bacterial strains in the biodegradation of diesel-polluted Antarctic soils at low environmental temperatures.

show abstract

Section: Introductionmentioning

confidence: 99%

Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…This behavior may be caused by the reported ability of Klebsiella oxytoca species to degrade cyanide in both aerobic and anaerobic environments (KAO et al, 2003;CHEN;KAO;CHEN, 2008). On the other hand, authors have previously analyzed the influence of inoculum size, temperature and pH on the degradation of cyanide by Serratia marcescens (KARAMBA et al, 2016); but, to our knowledge, this is the first report on the influence of rotation speed on the degradation of cyanide by Serratia marcescens.…”

Section: Influence Of Rotation Speed Incubationmentioning

confidence: 93%

“…and Klebsiella sp. strains could only tolerate cyanide concentrations up to 650mg.L -1 (SANKARANARAYANAN; GOWTHAMI, 2015) and up to 750 mg.L -1 for Serratia marcescens (KARAMBA et al, 2016), which means that isolated strains show potential for biotreating cyanide even in high concentrations.…”

Section: Susceptibility Assaysmentioning

confidence: 95%

“…Serratia marcescens has been previously reported as a cyanide-degrading bacteria in a study performed in Malaysia, isolated from soil and drainage water samples (KARAMBA et al, 2015) and then optimized for cyanide degradation using glucose and yeast extract as nutrients sources, incubated at 32.5˚C, pH 6 and with 200mg.L -1 of initial cyanide concentration (KARAMBA et al, 2016).…”

Section: % 56%mentioning

confidence: 99%

“…Bacillus strains (SKOWRONSKI;STROBEL, 1969;WU et al, 2014;KANDASAMY et al, 2015;RAZANAMAHANDRY et al, 2016). These strains are able to lower cyanide concentrations by using it in their metabolism and in different temperatures, pH, initial concentrations, or carbon source conditions (MEKUTO et al, 2015;KARAMBA et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Biological degradation of cyanide using native bacteria isolated from a cassava-processing effluent.

Arévalo¹,

Tania²

View full text Add to dashboard Cite

Cyanide (CN) can be present in the environment due to human activities including gold mining, electroplating, and nylon production. During the industrial processing of cassava, cyanide is liberated in effluents due to the presence of cyanogenic glycosides in the pulp. Cyanide is toxic for most living organisms since it inhibits the action of the cytochrome c oxidase and stops the electron transport chain during cellular respiration. Because of this, cyanide-containing effluents need to be treated prior to discharge to the environment, which can be achieved by chemical, physical or biological methods. In this context, this work evaluates the cyanide-degradation capacity of native bacteria isolated from a cassavaprocessing effluent and the best conditions for the biological removal of cyanide. In total, sixteen cyanide-resistant bacteria were isolated, identified by mass spectrometry technique MALDI-TOF, and tested individually for their ability to degrade cyanide. Eight different species were identified among the isolated strains. Four of them, belonging to the Klebsiella oxytoca, Bacillus pumilus, Corynebacterium glutamicum, and Serratia marcescens species, were selected for further assays to assess their susceptibility to pH and cyanide initial concentration. Results showed that cyanide degradation was inhibited when pH was above 9 and that higher initial cyanide concentrations resulted in lower cyanide degradation percentage. However, strains could still keep their degradation capacity even in high initial cyanide concentrations of 1000mg.L-1. Isolated Klebsiella oxytoca and Serratia marcescens were thus selected for optimization of the degradation process by analyzing the influence of carbon source, temperature and rotation speed. Both strains showed greater degradation rates when using sodium acetate as a carbon source and incubated at 30°C. Agitation speed did not show an effect on cyanide degradation by Klebsiella oxytoca, but enhanced degradation by Serratia marcescens strain. Under optimal incubation conditions, isolated Klebsiella oxytoca and Serratia marcescens strains could degrade cyanide from a synthetic 150mg.L-1 freecyanide solution, pH 8, using sodium acetate as a carbon source by 43 and 41% after 24hour of incubation, respectively, showing their potential to be used in the biodegradation of cyanide-containing effluents.

show abstract

Cyanide removal from aqueous environment by resting cells and PTFE immobilized cells of Sphingobacterium spp.

2021

View full text Add to dashboard Cite

Microbial detoxification of cyanide offered an inexpensive, safe, and viable alternative to physiochemical processes for the treatment of cyanide in industrial effluents or contaminated sites. This study involved isolation of novel strain with high resistance against cyanide toxicity and able to degrade the cyanide radical. The strain was isolated from rocky area and identified as Sphingobacterium multivorium using 16S ribosomal RNA. Resting pregrown cells were used in simple reaction mixture to avoid the complication associated with the media. One-gram fresh weight of this bacteria was able to remove 98.5% from 1.5 g/L cyanide which is a unique result. Factor affecting the biochemical process such as pH, temperature, agitation, glucose concentration was examined. The optimum conditions were, pH 6-7, 30-40°C, and 100-150 rpm shaking speed and 0.25% glucose. Furthermore, the cells were used after immobilization in polytetrafluoroethylene (PTFE) polymer. The PTFE is very safe carrier and the cells withstand the entrapment process and were able to remove 92% (1 g/L cyanide). The immobilized cells were used for six successive cycles with about 50% removal efficiency. The storage life extended to 14 days. No previous work studied the cyanide removal by Sphingobacterium spp. The strain showed good applicable characters.

show abstract

Optimisation of biodegradation conditions for cyanide removal by Serratia marcescens strain AQ07 using one-factor-at-a-time technique and response surface methodology

Cited by 45 publications

References 18 publications

Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica

Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica

Biological degradation of cyanide using native bacteria isolated from a cassava-processing effluent.

Cyanide removal from aqueous environment by resting cells and PTFE immobilized cells of Sphingobacterium spp.

Contact Info

Product

Resources

About