Optimisation of micropropagation protocols for temperate eucalypt hybrids in South Africa, with a focus on auxin transport proteins

Keret, Rafael; Nakhooda, Muhammad; Jones, N.B.; Hills, P.N.

doi:10.2989/20702620.2021.1987177

Cited by 3 publications

(6 citation statements)

References 69 publications

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“…Improved in vitro rooting in E. grandis × E. nitens was reported that, by reducing the strength of MS media from full to half strength and decreasing the concentration of sucrose in shooting media from 20 to 15 gL -1 . Additionally, it was observed that increasing the concentration of NAA from 0.1 mgL -1 to 0.5mgL -1 increased the average percentage of adventitious roots [29]. Also, increasing the IBA concentration from 0.1 mgL -1 to 0.5 mgL -1 increased root hair formation.…”

Section: Adventitious Root Formation and Root Hardeningmentioning

confidence: 91%

See 1 more Smart Citation

A Comprehensive Review Unleashing the Challenges and Advancements in In-Vitro Propagated Eucalypt Plantations

Sharma¹,

Ankita²,

Karnwal³

et al. 2023

Preprint

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The genus Eucalyptus is a globally captivated source of hardwood and is well known for its medicinal uses. The hybrid and wild species of Eucalyptus are widely used as exotic plantations due to their renowned potential of adapting to various systems and sites, furthermore, making the species the most extensively propagated. Despite unquestionable progression in biotechnological and tissue culture approaches, the productivity of plantations is still limited often due to the low efficiency of clonal propagation from cuttings. The obtained F1 hybrids yield high biomass and High-quality low-cost raw material for large-scale production, however, the development of hybrid, clonal multiplication, proliferation, and post-developmental studies are still major concerns. This riveting review describes the problems concerned with in-vitro and clonal propagation of Eucalypt plantation; recent advances in biotechnological and tissue culture practices for massive and rapid micro-propagation of Eucalyptus; as well, highlights the Eucalypt germplasm preservation techniques.

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Section: Adventitious Root Formation and Root Hardeningmentioning

confidence: 91%

“…Mercuric chloride has been reported to cause mammalian toxicity. So, the use of either Calcium hypochlorite or Sodium hypochlorite is recommended [28,29]. The basic explant sterilization Eucalypt is achieved by treating with Sodium hypochlorite at 67-1340 mM Concentrations.…”

Section: Establishing Axenic Culturementioning

confidence: 99%

A Comprehensive Review Unleashing the Challenges and Advancements in In-Vitro Propagated Eucalypt Plantations

Sharma¹,

Ankita²,

Karnwal³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Improved in vitro rooting in E. grandis × E. nitens was reported by reducing the strength of MS media from full to halfstrength and decreasing the concentration of sucrose in shooting media from 20 to 15 g L −1 . Additionally, it was observed that increasing the concentration of NAA from 0.1 mg L −1 to 0.5 mg L −1 increased the average percentage of adventitious roots [29]. Also, increasing the IBA concentration from 0.1 mg L −1 to 0.5 mg L −1 increased root hair formation.…”

Section: Adventitious Root Formation and Root Hardeningmentioning

confidence: 92%

“…Mercuric chloride has been reported to cause mammalian toxicity. So, the use of either calcium hypochlorite or sodium hypochlorite is recommended [28,29]. The basic explant sterilization of Eucalyptus is achieved by treating it with sodium hypochlorite at 67-1340 mM concentrations.…”

Section: Establishing Axenic Culturementioning

confidence: 99%

“…Finally, the plantlets were transferred to field conditions and showed 85-95% success rates in field conditions [17]. However, in another study, 58% of the success rate of plantlets in field conditions was due to the loss of some plantlets during handling [29]. Another innovative approach for acclimatization was reported, where the shoots were maintained in photoautotrophic culture at a high concentration of carbon dioxide and a low sugar concentration.…”

Section: Adventitious Root Formation and Root Hardeningmentioning

confidence: 99%

See 1 more Smart Citation

A Comprehensive Review Uncovering the Challenges and Advancements in the In Vitro Propagation of Eucalyptus Plantations

Sharma,

Ankita,

Karnwal

et al. 2023

Plants

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The genus Eucalyptus is a globally captivated source of hardwood and is well known for its medicinal uses. The hybrid and wild species of Eucalyptus are widely used as exotic plantations due to their renowned potential of adapting to various systems and sites, and rapid large-scale propagation of genetically similar plantlets, which further leads to the extensive propagation of this species. Tissue culture plays a crucial role in the preservation, propagation, and genetic improvement of Eucalyptus species. Despite unquestionable progression in biotechnological and tissue culture approaches, the productivity of plantations is still limited, often due to the low efficiency of clonal propagation from cuttings. The obtained F1 hybrids yield high biomass and high-quality low-cost raw material for large-scale production; however, the development of hybrid, clonal multiplication, proliferation, and post-developmental studies are still major concerns. This riveting review describes the problems concerning the in vitro and clonal propagation of Eucalyptus plantation and recent advances in biotechnological and tissue culture practices for massive and rapid micropropagation of Eucalyptus, and it highlights the Eucalyptus germplasm preservation techniques.

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The evolution of in vitro tracheary element systems from annual to perennial plant species

Keret

Hills

Drew

2023

Plant Cell Tiss Organ Cult

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Tracheary elements (TEs), including vessels and tracheids, occur as a product of xylogenesis and are highly adapted for the transportation of water and solutes. Xylogenesis or wood formation encompasses various stages of cellular development, which requires stringent temporal and spatial regulation. To further complicate matters, TEs are polymorphous and associated with other complex tissues. These complexities have necessitated the development of in vitro culture systems that are capable of synchronously inducing TEs on demand. In this review, we cover the challenges associated with inducing TEs in vitro and how this has been overcome using mesophyll and callus culture systems in herbaceous plants, yielding transdifferentiation efficiencies of up to 76% and 90%, respectively. We postulate that when equipped with such information, a great opportunity exists to optimise these culture systems in commercially valuable woody genera that currently display lower efficiencies in the range of 15.8–65%. Although both the mesophyll and callus induction cultures have proven essential for uncovering the fundamental processes associated with secondary growth, the mesophyll-based systems have recently become much less prominent (2.8x) in the literature compared to the callus-based systems. This is largely due to ease of application of the callus system to other plant species, paving the way for applications ranging from fundamental research in economically valuable woody genera to the 3D-printing of biomaterial products in vitro.

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Optimisation of micropropagation protocols for temperate eucalypt hybrids in South Africa, with a focus on auxin transport proteins

Cited by 3 publications

References 69 publications

A Comprehensive Review Unleashing the Challenges and Advancements in In-Vitro Propagated Eucalypt Plantations

A Comprehensive Review Unleashing the Challenges and Advancements in In-Vitro Propagated Eucalypt Plantations

A Comprehensive Review Uncovering the Challenges and Advancements in the In Vitro Propagation of Eucalyptus Plantations

The evolution of in vitro tracheary element systems from annual to perennial plant species

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