Optimization and Characterization of Peg-PCL-Peg Triblock Copolymer as Carrier of Drug Using Full Factorial Design

Abstract

Objective: Triblock copolymer of poly(ethylene glycol)-poly(ɛ-caprolactone)-poly(ethylene glycol) (PEG-PCL-PEG, PECE) was applicated as hydrophobic drug. This study aims to optimization and characterization of PECE triblock copolymer as carriers of hydrophobic drug (ketoprofen).Methods: Triblock copolymer of PECE was prepared with varying composition ratio of PEG and PCL by ring-opening and coupling reaction. The characteristics of triblock copolymer were characterized using FTIR and DSC. Variation composition ratio of poly(ɛ-caprolactone) (PCL)/poly(ethylene glycol) (PEG) and ratio PECE/drug as factors for optimization using full factorial design. Ketoprofen was loaded into PECE triblock copolymer micelles by emulsification and solvent evaporation method. Responses were measured particle size, entrapment efficiency (EE) and drug solubility. Results:The result of this study showed that a higher ratio of PCL/PEG and ratio of PECE/drug, reducing particle size, increasing EE and improving drug solubility. The optimum formula obtained by ratio of PCL/PEG is 2:1 and ratio of PECE/drug is 40:1 with particle size is 356,967±9,142 nm, EE is 57,751±0,437%, drug solubility is 32,648±0,200 µg/ml and zeta potential-18,867±2,578 mV. A full factorial design was applied to determine the optimum formula for the PECE triblock copolymer as drug carriers. Conclusion:The PECE triblock copolymer was preparated using ring-opening polymerization method with Sn(Oct)2 as a catalyst and then continued the reaction with HMDI as coupling agent. Ketoprofen was loaded into PECE triblock copolymer using methods emulsification and solvent evaporation.

Optimization of Rivastigmine Chitosan Nanoparticles for Neurodegenerative Alzheimer; In Vitro and Ex Vivo Characterizations

“…Chitosan nanoparticles were prepared using ionotropic gelation of chitosan with sodium tripolyphosphate STPP anions [18] with some modification. Chitosan was dissolved in purified water at various ratios 0.5% w/v, 0.7% w/v, 1% w/v, 1.2% w/v and 1.4% w/v.…”

“…18 °C was shown by endothermic peak. Clear glass transition endothermic peak was observed at 73.38 °C.…”

The improvement potential of camel milk whey as a natural remedy in comparison with Rivastigmine chitosan-loaded nanoparticles in aluminum chloride induced Alzheimer-like disease in rats

“…Desperation of Rivastigmine (RS) harbored on optimized chitosan nanoparticles as polymeric Nanocarriers was prepared. The formulation of chitosan nanoparticles (CSNPs) was done by ionotropic gelation of chitosan using sodium tripolyphosphate STPP anions [17]. The detailed preparation procedures with some methodology modification and the choices of the optimal formula have been explained in previous published research [18].…”