Optimization and Characterization of Poly[R]hydroxyalkanoates of Pseudomonas aeruginosa

Samrot, Antony V.; Bhakyalakshmi, M; Venkatraman, K. L; Sahiti, K; Philip, Sheryl-Ann; Jahnavi, T; Senthilkumar, P.

doi:10.13005/bbra/1883

Cited by 3 publications

(3 citation statements)

References 8 publications

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“…Polyhydroxyalkanoates (PHAs) belong to the polyester family that contains a unique bond of esters, accumulated as energy and carbon reserve with a deficient nitrogen source, and provide necessary energy. − PHAs are composed of 3-hydroxy acid monomers, − as shown in Table . The n value commonly varies between 100 and 30 000 .…”

Section: Polyhydroxyalkanoate Polymersmentioning

confidence: 99%

Recent Development of Polyhydroxyalkanoates (PHA)-Based Materials for Antibacterial Applications: A Review

Ladhari

Boisvert

et al. 2023

ACS Appl. Bio Mater.

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The diseases caused by microorganisms are innumerable existing on this planet. Nevertheless, increasing antimicrobial resistance has become an urgent global challenge. Thus, in recent decades, bactericidal materials have been considered promising candidates to combat bacterial pathogens. Recently, polyhydroxyalkanoates (PHAs) have been used as green and biodegradable materials in various promising alternative applications, especially in healthcare for antiviral or antiviral purposes. However, it lacks a systematic review of the recent application of this emerging material for antibacterial applications. Therefore, the ultimate goal of this review is to provide a critical review of the state of the art recent development of PHA biopolymers in terms of cutting-edge production technologies as well as promising application fields. In addition, special attention was given to collecting scientific information on antibacterial agents that can potentially be incorporated into PHA materials for biological and durable antimicrobial protection. Furthermore, the current research gaps are declared, and future research perspectives are proposed to better understand the properties of these biopolymers as well as their possible applications.

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Section: Polyhydroxyalkanoate Polymersmentioning

confidence: 99%

Recent Development of Polyhydroxyalkanoates (PHA)-Based Materials for Antibacterial Applications: A Review

Ladhari

Boisvert

et al. 2023

ACS Appl. Bio Mater.

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“…Polyhydroxyalkanoates (PHAs) are polyesters that contain a characteristic bond of esters, which are accumulated as carbon and energy reserve along with limited nitrogen source and assist in providing energy [ 1 , 2 , 3 , 4 , 5 ]. The structure of PHAs is composed of 3-hydroxy fatty acid monomers [ 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

The Synthesis, Characterization and Applications of Polyhydroxyalkanoates (PHAs) and PHA-Based Nanoparticles

et al. 2021

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Polyhydroxyalkanoates (PHAs) are storage granules found in bacteria that are essentially hydroxy fatty acid polyesters. PHA molecules appear in variety of structures, and amongst all types of PHAs, polyhydroxybutyrate (PHB) is used in versatile fields as it is a biodegradable, biocompatible, and ecologically safe thermoplastic. The unique physicochemical characteristics of these PHAs have made them applicable in nanotechnology, tissue engineering, and other biomedical applications. In this review, the optimization, extraction, and characterization of PHAs are described. Their production and application in nanotechnology are also portrayed in this review, and the precise and various production methods of PHA-based nanoparticles, such as emulsion solvent diffusion, nanoprecipitation, and dialysis are discussed. The characterization techniques such as UV-Vis, FTIR, SEM, Zeta Potential, and XRD are also elaborated.

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“…Maximal PHA production depend on growth, cell type, cell density etc. even the physiochemical factors like incubation time, carbon source, temperature, pH have much influence in PHA production ( [12,13]. Cost for the production of PHA using chemicals is much expensive [14].…”

Section: Introductionmentioning

confidence: 99%

Utilization of palm oil mill effluent and clindamycin for optimization of polyhydroxy [r] alkanoates production

2019

Biointerface Res Appl Chem

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Polyhydroxyalkanoates (PHA) are storage granules of most bacteria which can be used as biodegradable plastics but the production cost of PHA is twice than petrochemical based synthetic polymers because of substrate cost. The use of alternative renewable and cheap carbon sources are the best option, one such is palm oil mill effluent (POME). POME contains carbon source like volatile fatty acids and other organic components which can be utilised by microorganisms to accumulate PHA. The use of subinhibitory concentration of antibiotics like clindamycin may have an influence on PHA accumulation. In this study, 31 organisms were isolated from POME spillage area and subjected to PHA production. Seven organisms were found to accumulate PHA, which was confirmed by Nile blue staining method, the accumulated PHA was extracted and characterized using HPLC. All the organisms were found to produced poly hydroxy butyrate (PHB). Amongst all the seven isolates, two organisms namely Bacillus sp and Pseudomonas aeruginosa were found to accumulate more PHA. Both the organisms were subjected to produce PHA in POME and clindamycin containing media. PHA production condition was optimized using RSM.

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Optimization and Characterization of Poly[R]hydroxyalkanoates of Pseudomonas aeruginosa

Cited by 3 publications

References 8 publications

Recent Development of Polyhydroxyalkanoates (PHA)-Based Materials for Antibacterial Applications: A Review

Recent Development of Polyhydroxyalkanoates (PHA)-Based Materials for Antibacterial Applications: A Review

The Synthesis, Characterization and Applications of Polyhydroxyalkanoates (PHAs) and PHA-Based Nanoparticles

Utilization of palm oil mill effluent and clindamycin for optimization of polyhydroxy [r] alkanoates production

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