Optimization and comparison of HPLC and RRLC conditions for the analysis of carbonyl-DNPH derivatives

Ochs, Soraya de Mendonça; Fasciotti, Maíra; Barreto, R. P.; Figueiredo, Natália Guimarães de; Albuquerque, Flávio Cortiñas; Massa, M. Cecília G. Pontes; Gabardo, Irene Terezinha; Netto, Annibal Duarte Pereira

doi:10.1016/j.talanta.2009.12.036

Cited by 24 publications

(3 citation statements)

References 37 publications

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“…The recent development of liquid chromatographic columns using sub-2-µm particles and ultra-high pressure allow rapid resolution liquid chromatography (RRLC or UPLC or UHPLC) to show significant improvements in resolution, analysis speed, detection sensitivity, and reduction of solvent waste compared to HPLC analysis [10]. In the present study, a RRLC fingerprint analysis method was developed to compare the chemical constituents and the relative amounts of those constituents in Heshouwu and Zhi-heshouwu.…”

Section: Comparative Analyses Of Chromatographic Fingerprints Of the mentioning

confidence: 99%

“…The constituents of herbs are known to vary significantly depending on their cultivation conditions (e.g., region, soil, and temperature), drying processes, and harvest seasons [21,22]. Therefore, 15 batches of Heshouwu samples ( [1][2][3][4][5][6][7][8][9][10][11][12][13][14] purchased from different provinces of China were investigated. The contents of TTSG in all samples should be higher than 1.0 % and 0.70 % in Heshouwu and Zhi-heshouwu, respectively, to meet to the requirements of the Chinese Pharmacopoeia [1].…”

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Comparative Analyses of Chromatographic Fingerprints of the Roots ofPolygonum multiflorumThunb. and Their Processed Products Using RRLC/DAD/ESI-MSⁿ

Liu

Liu²,

Wang³

et al. 2011

Planta Med

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The dried roots of Polygonum multiflorum Thunb. (Heshouwu) and their processed products (Zhi-heshouwu) are widely used in traditional Chinese medicine, yet their therapeutic effects are different. Previous investigations focused mainly on the differences between Heshouwu and Zhi-heshouwu in the contents of several known compounds. In this study, a rapid resolution liquid chromatography-diode array detection/electrospray ionization tandem mass spectrometry (RRLC/DAD/ESI-MS(n)) method was developed for the comparative analysis of the components of Heshouwu and Zhi-heshouwu. A total of 23 compounds were identified or tentatively characterized. We found that 16 batches of Heshouwu and 15 batches of Zhi-heshouwu samples shared eight compounds, including gallic acid; 3,5,4'-tetrahydroxylstilbene-2,3-di-O-glucoside, CIS-2,3,5,4'-tetrahydroxylstilbene-2-O- β-D-glucoside, trans-2,3,5,4'-tetrahydroxylstilbene-2-O- β-D-glucoside, emodin-8-O- β-D-glucoside, physcion-8-O- β-D-glucoside, emodin, and physcion. Nevertheless, the relative amounts of gallic acid, emodin, and physcion were very high in Zhi-heshouwu samples compared to those in Heshouwu samples. Six compounds disappeared after processing and were unique for Heshouwu: catechin, flavanol gallate dimer, polygonimitin B, emodin-1-O-glucoside, emodin-8-O-(6'-O-malonyl)-glucoside, and physcion-8-O-(6'-O-malonyl)-glucoside. Three compounds were unique for Zhi-heshouwu: hydroxymaltol, 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one, and 5-hydroxymethyl furfural. These results suggest that the types and relative amounts of the chemical components of Heshouwu and Zhi-heshouwu are different.

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Section: Comparative Analyses Of Chromatographic Fingerprints Of the mentioning

confidence: 99%

mentioning

confidence: 99%

Comparative Analyses of Chromatographic Fingerprints of the Roots ofPolygonum multiflorumThunb. and Their Processed Products Using RRLC/DAD/ESI-MSⁿ

Liu

Liu²,

Wang³

et al. 2011

Planta Med

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show abstract

“…De todas las técnicas mencionadas, la cromatografía de líquidos de alta resolución es, por su alta sensibilidad y selectividad, rápida y fácil de usar [4,11] en determinaciones analíticas, así como la técnica más utilizada para separar el formaldehído de posibles interferencias y mejorar el límite de detección. Por otro lado, con respecto a lo que a tratamiento de muestra se refiere, es el procedimiento de derivatización que se basa en la reacción de las muestras con la 2,4-dinitrofenilhidracina (dnfh) por su selectividad de reacción con aldehídos y cetonas, al formar compuestos coloreados estables de tipo hidrazonas [4,[11][12][13][14][15], el utilizado con más frecuencia.…”

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Uso de CLAR en fase inversa para la determinación del contenido de formaldehído libre en esmaltes de uñas

et al. 2017

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Introducción: este artículo es producto de la investigación titulada “Uso de clar en fase inversa para la determinación del contenido de formaldehído libre en esmaltes de uñas”, desarrollada en el 2015 en el Centro de Innovación Aplicada en Tecnologías Competitivas (ciatec). Metodología: se determinó el contenido de formaldehído libre en 50 muestras de esmaltes para uñas de diversas marcas comercializadas en León, Guanajuato, México. El tratamiento de muestras se realizó de acuerdo con el método iso 17226-1:2003, con ligeras modificaciones: se pesaron 2±0,1 g de esmaltes en matraces Erlenmeyer de 100 ml, se adicionaron 40 ml de dodecil sulfonato de sodio al 0,1 %. Las muestras fueron entonces tapadas y se dejaron en baño María a 40 °c con agitación constante durante 60 minutos; posteriormente se filtraron y se tomaron alícuotas de cada muestra para su derivatización con 2,4-dinitrofenilhidracina al 0,3 % durante 60 minutos. La determinación analítica se realizó por clar en fase inversa con detección por arreglo de diodos. Resultados: se obtuvo como resultado que todas las muestras de esmaltes para uñas analizadas estuvieron por debajo del límite normativo de contenido de formaldehído libre en cosméticos de 0,2 %. Conclusiones: se obtuvo información de relevancia toxicológica sobre el formaldehído libre en los esmaltes de uñas, además de comprobar la versatilidad y robustez del método de prueba.

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Monitoring of radical scavenging compounds from oxidized lipids (RSOL) by 2,4-dinitrophenylhydrazine (DNPH) derivatization in thermally oxidized linoleic acid and lard systems

Jeong

Yeo

Jang

et al. 2013

Food Sci Biotechnol

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Optimization and comparison of HPLC and RRLC conditions for the analysis of carbonyl-DNPH derivatives

Cited by 24 publications

References 37 publications

Comparative Analyses of Chromatographic Fingerprints of the Roots ofPolygonum multiflorumThunb. and Their Processed Products Using RRLC/DAD/ESI-MSⁿ

Comparative Analyses of Chromatographic Fingerprints of the Roots ofPolygonum multiflorumThunb. and Their Processed Products Using RRLC/DAD/ESI-MSⁿ

Uso de CLAR en fase inversa para la determinación del contenido de formaldehído libre en esmaltes de uñas

Monitoring of radical scavenging compounds from oxidized lipids (RSOL) by 2,4-dinitrophenylhydrazine (DNPH) derivatization in thermally oxidized linoleic acid and lard systems

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Optimization and comparison of HPLC and RRLC conditions for the analysis of carbonyl-DNPH derivatives

Cited by 24 publications

References 37 publications

Comparative Analyses of Chromatographic Fingerprints of the Roots ofPolygonum multiflorumThunb. and Their Processed Products Using RRLC/DAD/ESI-MSn

Comparative Analyses of Chromatographic Fingerprints of the Roots ofPolygonum multiflorumThunb. and Their Processed Products Using RRLC/DAD/ESI-MSn

Uso de CLAR en fase inversa para la determinación del contenido de formaldehído libre en esmaltes de uñas

Monitoring of radical scavenging compounds from oxidized lipids (RSOL) by 2,4-dinitrophenylhydrazine (DNPH) derivatization in thermally oxidized linoleic acid and lard systems

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Product

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Comparative Analyses of Chromatographic Fingerprints of the Roots ofPolygonum multiflorumThunb. and Their Processed Products Using RRLC/DAD/ESI-MSⁿ

Comparative Analyses of Chromatographic Fingerprints of the Roots ofPolygonum multiflorumThunb. and Their Processed Products Using RRLC/DAD/ESI-MSⁿ