Optimization, antioxidant properties and GC–MS analysis of Periploca angustifolia polysaccharides and chelation therapy on cadmium-induced toxicity in human HepG2 cells line and rat liver

Athmouni, Khaled; Belhaj, Dalel; Feki, Abdelfattah El; Ayadi, Habib

doi:10.1016/j.ijbiomac.2017.10.175

Cited by 33 publications

(21 citation statements)

References 43 publications

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“…The antioxidants capacity and efficacy of polysaccharides can be assessed more accurately indicating Cd-induced hepatotoxicity. Increased ALAT, ASAT and biliburin activities might be attributed to hepatic structural damage, causing release of these enzymes into the circulation [5]. However, the liver ALAT, ASAT and bilirubin activities were markedly (P < 0.05) reduced in rats treated with CFv-PS, suggesting that CFv-PS effectively conditioned the hepatocytes, maintained hepatic structural integrity, decreased leakage of the enzymes into circulation and alleviated Cd-induced liver injury.…”

Section: Discussionmentioning

confidence: 97%

“…Cd is an environmental pollutant extremely toxic to human and animals. Owing to its long half- neurodegenerative and heart diseases, carcinogenesis diabetes and liver injury [5,38,39]. The delicate balance between ROS production and clearance is critical to maintain healthy cellular physiology [40].…”

Section: Discussionmentioning

confidence: 99%

“…In order to understand the hepatoprotective activity of CFv-PS in vivo, both antioxidant and oxidant systems were examined. Hepatocellular damage can be triggered by Cd through over generation of free radicals [5], which react with proteins or lipids leading to their peroxidation [50]. Therefore, Cd-induced liver injury is closely associated with the formation of free radicals and antioxidants can reduce the toxicity of Cd.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, Cd-induced liver injury is closely associated with the formation of free radicals and antioxidants can reduce the toxicity of Cd. A series of antioxidant enzymes (SOD, CAT and GPx) and non-enzymatic antioxidants (Metallothionein, Vitamin C) are regarded as important defense systems against oxidative stress [5].…”

Section: Discussionmentioning

confidence: 99%

“…Cd can be absorbed through food chain, and then accumulated in organs particularly in liver which is the metabolic dock of its entry and the major destination of its toxicity. The injury of liver induced by Cd depends on the dose and duration of Cd exposure, as previous research suggested that low Cd dose exposure led to pathological changes in the liver tissues [5]. With acute or chronic Cd, significant DNA damage was also observed [6].…”

Section: Introductionmentioning

confidence: 94%

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Polysaccharides from Phormidium versicolor (NCC466) protecting HepG2 human hepatocellular carcinoma cells and rat liver tissues from cadmium toxicity: Evidence from in vitro and in vivo tests

Belhaj

Athmouni

Aoiadni

et al. 2018

International Journal of Biological Macromolecules

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The in vitro antioxidant, cytotoxic and cytoprotective properties and in vivo hepatoprotective activities of crude polysaccharides extracted from cyanobacteria Phormidim versicolor NCC466 (CFv-PS) were investigated. The CFv-PS, identified as heteropolysaccharides with molecular weight of 63.79kDa, exhibited relatively strong antioxidant activity, in a concentration-depended manner, in vitro assays. Additionally, CFv-PS did not induce cytotoxic effect on HepG2 human hepatocellular carcinoma cells within the range of tested concentrations (25-150μg·mL) while preventing them against Cd. Moreover, in rats subjected to Cd-induced hepatotoxicity, CFv-PS pretreatment significantly (P<0.05) reduced the level of ALAT, ASAT, biliburin, MDA, protein carbonyl and DNA damage, and markedly increased enzyme activities in liver tissues. These findings suggest that the cyanobacteria Phormidium versicolor is a potential source of natural products possessing antioxidant, cytoprotective and hepatoprotective properties.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

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Polysaccharides from Phormidium versicolor (NCC466) protecting HepG2 human hepatocellular carcinoma cells and rat liver tissues from cadmium toxicity: Evidence from in vitro and in vivo tests

Belhaj

Athmouni

Aoiadni

et al. 2018

International Journal of Biological Macromolecules

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Morphology, Solid Structure and Antioxidant Activity in Vitro of Angelica sinensis Polysaccharide‐Ce(IV)

Wang

Huang

et al. 2023

Chemistry & Biodiversity

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Angelica Sinensis polysaccharide cerium (ASP−Ce) was prepared by Angelica Sinensis polysaccharide (ASP) and cerium ammonium nitrate (NH4)2Ce(NO3)6. and its morphology, and solid structure was investigated. The antioxidant activity of the ASP−Ce complex in vitro was evaluated. The antioxidant activity of ASP−Ce complex in vitro was evaluated by the scavenging activity of 2,2‐diphenyl‐1‐picrylhyrazyl radical (DPPH), hydroxyl radical (⋅OH) and superoxide anion radical ( normalO2- ${{{\rm O}}_{2}^{-}}$ ⋅). The results showed that the ASP−Ce had the more ordered structure for inserting the Ce4+ into the polymer chain of ASP and there was little change in the conformation of the polysaccharide from Ce4+. Three free radical scavenging experiments proved that ASP−Ce had better antioxidant capacity than of ASP, especially on DPPH, and then on normalO2- ${{{\rm O}}_{2}^{-}}$ ⋅. The scavenging rate of ASP−Ce at 1.0 mg/mL on DPPH reached 71.6 %. Therefore, these results provide references for the further development and utilization of rare earth‐polysaccharide.

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The effects of pretreatment with lithium metaborate dihydrate on lipid peroxidation and Ca, Fe, Mg, and K levels in serum of Wistar albino male rats exposed to Cd

Taşdemir

Çelikezen

Oto

et al. 2019

Environ Sci Pollut Res

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Optimization, antioxidant properties and GC–MS analysis of Periploca angustifolia polysaccharides and chelation therapy on cadmium-induced toxicity in human HepG2 cells line and rat liver

Cited by 33 publications

References 43 publications

Polysaccharides from Phormidium versicolor (NCC466) protecting HepG2 human hepatocellular carcinoma cells and rat liver tissues from cadmium toxicity: Evidence from in vitro and in vivo tests

Polysaccharides from Phormidium versicolor (NCC466) protecting HepG2 human hepatocellular carcinoma cells and rat liver tissues from cadmium toxicity: Evidence from in vitro and in vivo tests

Morphology, Solid Structure and Antioxidant Activity in Vitro of Angelica sinensis Polysaccharide‐Ce(IV)

The effects of pretreatment with lithium metaborate dihydrate on lipid peroxidation and Ca, Fe, Mg, and K levels in serum of Wistar albino male rats exposed to Cd

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