Optimization for size separation of graphene oxide sheets by flow/hyperlayer field-flow fractionation

Ko, Myoungjae; Choi, Hee Jae; Kim, Jin Yong; Kim, In Ho; Kim, Sang Ouk; Moon, Myeong Hee

doi:10.1016/j.chroma.2022.463475

Cited by 1 publication

(2 citation statements)

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“…Parabolic flow is characterized by maximum velocities in the center of the channel decreasing until reaching zero at the walls [5]. Based on how the analytes are distributed across the channel, different operating modes [6,7] are distinguished (Figure 2):…”

Section: Introduction 1fff Techniquesmentioning

confidence: 99%

“…The elution order is still reversed compared to normal mode, but retention depends not solely on the size of the analytes (like in steric mode) but also other physical features (e.g., shape, rigidity, density, surface properties). Based on how the analytes are distributed across the channel, different operating modes [6,7] are distinguished (Figure 2): Based on how the analytes are distributed across the channel, different operating modes [6,7] are distinguished (Figure 2):…”

Section: Introduction 1fff Techniquesmentioning

confidence: 99%

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Field-Flow Fractionation in Molecular Biology and Biotechnology

Giordani,

Marassi,

Placci

et al. 2023

Molecules

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Field-flow fractionation (FFF) is a family of single-phase separative techniques exploited to gently separate and characterize nano- and microsystems in suspension. These techniques cover an extremely wide dynamic range and are able to separate analytes in an interval between a few nm to 100 µm size-wise (over 15 orders of magnitude mass-wise). They are flexible in terms of mobile phase and can separate the analytes in native conditions, preserving their original structures/properties as much as possible. Molecular biology is the branch of biology that studies the molecular basis of biological activity, while biotechnology deals with the technological applications of biology. The areas where biotechnologies are required include industrial, agri-food, environmental, and pharmaceutical. Many species of biological interest belong to the operational range of FFF techniques, and their application to the analysis of such samples has steadily grown in the last 30 years. This work aims to summarize the main features, milestones, and results provided by the application of FFF in the field of molecular biology and biotechnology, with a focus on the years from 2000 to 2022. After a theoretical background overview of FFF and its methodologies, the results are reported based on the nature of the samples analyzed.

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Section: Introduction 1fff Techniquesmentioning

confidence: 99%