Optimization of a GF‑AAS method for lead testing in blood and urine: A useful tool in acute abdominal pain management in emergency

Tudosie, M; Caragea, Genica; Popescu, Dragos Marian; Avram, Oana; Şerban, Dragoş; Smarandache, Cătălin Gabriel; Tudor, Corneliu; Badiu, Cristinel Dumitru; Socea, Bogdan; Sabău, Alexandru Dan; Comandasu, Meda; Spataru, Radu Iulian; Costea, Daniel Ovidiu; Tănăsescu, Ciprian; Dascălu, Ana Maria

doi:10.3892/etm.2021.10417

Cited by 4 publications

(1 citation statement)

References 33 publications

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“…Accurate detection of bioavailable Pb in soil and water is necessary to predict its ecological risk ( Roux and Marra, 2007 ; Kumar et al, 2020 ). From available studies, most methods for detecting Pb rely on expensive instruments that are not portable and limited to specific analytical systems ( Klotz and Goen, 2017 ; Tudosie et al, 2021 ; Manousi et al, 2022 ). Although the mainstream instrumental methods are sensitive and accurate, the analytical results mainly reflect the total amount of elemental Pb.…”

Section: Introductionmentioning

confidence: 99%

Pb(II)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead

Zhu

Guo²,

et al. 2023

Front. Microbiol.

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With the rapid development of synthetic biology, various whole-cell biosensors have been designed as valuable biological devices for the selective and sensitive detection of toxic heavy metals in environmental water. However, most proposed biosensors are based on fluorescent and bioluminescent signals invisible to the naked eye. The development of visible pigment-based biosensors can address this issue. The pbr operon from Klebsiella pneumoniae is selectively induced by bioavailable Pb(II). In the present study, the proviolacein biosynthetic gene cluster was transcriptionally fused to the pbr Pb(II) responsive element and introduced into Escherichia coli. The resultant biosensor responded to Pb(II) in a time- and dose-dependent manner. After a 5-h incubation with Pb(II), the brown pigment was produced, which could be extracted into n-butanol. Extra hydrogen peroxide treatment during n-butanol extract resulted in the generation of a stable green pigment. An increased brown signal was observed upon exposure to lead concentrations above 2.93 nM, and a linear regression was fitted from 2.93 to 3,000 nM. Extra oxidation significantly decreased the difference between parallel groups. The green signal responded to as low as 0.183 nM Pb(II), and a non-linear regression was fitted in a wide concentration range from 0.183 to 3,000 nM. The specific response toward Pb(II) was not interfered with by various metals except for Cd(II) and Hg(II). The PV-based biosensor was validated in monitoring bioaccessible Pb(II) spiked into environmental water. The complex matrices did not influence the regression relationship between spiked Pb(II) and the dual-color signals. Direct reading with the naked eye and colorimetric quantification enable the PV-based biosensor to be a dual-color and low-cost bioindicator for pollutant heavy metal.

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Section: Introductionmentioning

confidence: 99%

Pb(II)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead

Zhu

Guo²,

et al. 2023

Front. Microbiol.

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Recent advances in graphite furnace atomic absorption spectrometry: a review of fundamentals and applications

Butcher

2023

Applied Spectroscopy Reviews

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Lead poisoning as a differential diagnosis in pediatric patients with chronic abdominal pain: a case–control study in Tehran-Iran

Mohsenipour,

Aflatoonian,

Alimadadi

et al. 2024

BMC Gastroenterol

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Background Chronic abdominal pain is a potential symptom of lead poisoning, which is often challenging to diagnose. This case–control study aimed to evaluate blood lead levels in pediatric patients with chronic abdominal pain. Methods The case–control study was conducted on 190 pediatrics who presented to the Children's Medical Center Hospital clinics, Tehran between April 2021- 2023. The children were divided into two groups: the case group, consisting of 81 patients with chronic abdominal pain, and the matched control group; 109 children without any gastrointestinal symptoms. The statistical analysis of the data was performed using STATA 16. A multiple logistic regression model was used to assess the association of different independent variables with chronic abdominal pain. Results There was no significant difference between mean (± standard deviation [SD]) of age (8.80(2.7) years vs. control group: 9.23(3.9) years), sex, and BMI (16.55(4.6) vs. 17.32(4.7)) of the patients with chronic abdominal pain (case group) and the control group, whereas the mean weight was remarkably low in patients with chronic abdominal pain: 27.25(± 12.1) kg vs. 31.70(± 14.7) kg ( P value = 0.028). Fifty-nine percent of children with chronic abdominal pain had serum lead levels ≥ 10 µg/dL. The mean (SD) of blood lead levels was statistically high in the case group: 11.09 (± 5.35) µg/dL vs. control group: 8.26 (± 5.01) µg/dL) ( P value ≤ 0.05). The appetite level was significantly low in the case group: 3.8 (± 2.5) vs. control group 5.4 (± 1.3). Conclusions Lead poisoning could be a possible cause of children's chronic abdominal pain. Regarding the high rate of lead poisoning in children exerting appropriate measures to reduce their exposure to lead is necessary. Supplementary Information The online version contains supplementary material available at 10.1186/s12876-024-03337-1.

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Optimization of a GF‑AAS method for lead testing in blood and urine: A useful tool in acute abdominal pain management in emergency

Cited by 4 publications

References 33 publications

Pb(II)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead

Pb(II)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead

Recent advances in graphite furnace atomic absorption spectrometry: a review of fundamentals and applications

Lead poisoning as a differential diagnosis in pediatric patients with chronic abdominal pain: a case–control study in Tehran-Iran

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