Optimization of a growth medium using a statistical approach for the production of an alkaline protease from a newly isolated Bacillus sp. L21

Tarı, Canan; Gençkal, Hande; Tokatlı, Figen

doi:10.1016/j.procbio.2005.08.012

Cited by 91 publications

(53 citation statements)

References 21 publications

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“…Factors like carbon and nitrogen sources and their concentrations have always been of great interest to the researchers in the industry for the low cost media design especially when strains lacking information on their growth requirements, are under consideration such as the case here [22,23]. It is also known that 30-40% of the production cost of industrial enzymes is estimated to be the cost of growth medium [24].…”

Section: Resultsmentioning

confidence: 99%

Optimization of biomass, pellet size and polygalacturonase production by Aspergillus sojae ATCC 20235 using response surface methodology

Tarı

Gogus

Tokatlı

2007

Enzyme and Microbial Technology

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A two-step optimization procedure using central composite design with four factors (concentrations of maltrin and corn steep liquor (CSL), agitation speed and inoculation ratio) was used in order to investigate the effect of these parameters on the polygalacturonase (PG) enzyme activity, mycelia growth (biomass) and morphology (pellet size) of Aspergillus sojae ATCC 20235. According to the results of response surface methodology (RSM), initial concentrations of maltrin and CSL and agitation speed were significant (p < 0.05) on both PG enzyme production and biomass formation. As a result of this optimization, maximum PG activity (13.5 U/ml) was achievable at high maltrin (120 g/l), at low CSL (0 g/l), high agitation speed (350 rpm) and high inoculation ratio (2 × 10 7 total spore). Similarly, maximum biomass (26 g/l) could be obtained under the same conditions with only the difference for higher level of CSL requirement. The diameter of pellets in all optimization experiments ranged between 0.05 and 0.76 cm. The second optimization step improved the PG activity by 74% and the biomass by 40%.

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Section: Resultsmentioning

confidence: 99%

Optimization of biomass, pellet size and polygalacturonase production by Aspergillus sojae ATCC 20235 using response surface methodology

Tarı

Gogus

Tokatlı

2007

Enzyme and Microbial Technology

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“…Soybean meal at 3 g/l, maltose 50 between the ranges of 30 and 40 g/l and tween 80 at 0.35 g/l produced an activity of 307 U/ml, which corresponded to 40% increase. [23].…”

Section: Characterization Of the Crude Enzyme Obtained From L21mentioning

confidence: 99%

Alkaline protease production from alkalophilic Bacillus sp. isolated from natural habitats

Gençkal

Tarı

2006

Enzyme and Microbial Technology

124

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Bacillus strains isolated under extreme alkaline conditions (Izmir, Turkey), were screened and identified for high alkaline protease activity. Strains with high protease yields were optimized with respect to inoculum concentration, temperature, agitation speed, initial medium pH and incubation time. Three Bacillus strains coded as I18, L18 and L21 showed high potential, for alkaline protease activity (160-222 U/ml) among 85 isolates. The specific growth rates were estimated from the growth curves as 0.49 h −1 for I18, as 0.6 and 0.7 h −1 for L18 and L21, respectively. The optimum temperatures were determined as 30 • C for strain I18 and 37 • C for the strains L18 and L21. Similarly, the optimum agitation speeds were 100 rpm for I18 and 180 rpm for L18 and L21. For all three strains, the optimum inoculation ratio and incubation time, were determined as 5% (v/v) and 96 h, respectively. The optimum initial media pH was found as pH 10 for strain L18 and L21. Bacillus sp. L21 with the highest specific protease activity (60 U/mg protein) and a broader pH range was chosen for further study. The biomass and product yield for this strain was determined as 0.023 g cell/g glucose and 0.021 U/g glucose, respectively. The crude enzyme of this strain was further characterized and was determined as a bleach stable, serine alkaline protease with an optimum temperature of 60 • C and a pH of 11, with a potential to be a candidate for the applications in the detergent industry.

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“…[33], soybean meal and Bacillus sp. L21 [34]. The proven ability of bacteria to grow and to produce sizable levels of protease from various wastes and agro-industrial substrates offers tremendous potential for developing biotechnological methods for hydrolysis of such by-products.…”

Section: Bacillus Pumilus Strain Cicchlj Q89mentioning

confidence: 99%

A Bacillus Strain Able to Hydrolyze Alpha- and Beta-Keratin

Fellahi

Zaghloul²,

Feuk-Lagerstedt³

et al. 2014

J Bioproces Biotech

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The ability to hydrolyze keratin, a rigid and strongly cross-linked protein in the waste of poultry feather and sheep wool, has made keratinase production by microorganisms highly important to the biotechnological industry. A proteindegrading bacterium (C 4 ) was isolated from compost. Based on morphology and biochemical tests, along with 16S rRNA sequencing, the isolated C 4 was tentatively identified as Bacillus sp. C 4 (2008). The proteolytic activity of the Bacillus sp. C 4 strain was broadly specific; it degraded keratinous and non-keratinous proteins to different degrees. Pea pods as substrate generated the highest protease production, followed by soybean meal and sheep wool. Notwithstanding, using wool keratin as a sole source of carbon and nitrogen yielded the highest level of soluble proteins. Furthermore, the C 4 bacterium grew well, and produced a significant level of keratinase when using wool and feather as substrates. Supplementing the medium with yeast extract and peptone shortened the time required for feather degradation, but delayed the onset of the wool keratin hydrolysis with two days. The predominant amino acids released in feather hydrolysate were tyrosine, phenylalanine, and histidine. In contrast, the wool lysate was rich in aspartic acid, methionine, tyrosine, phenylalanine, histidine, and lysine. Results established that utilizing the C 4 strain for keratin degradation in waste management holds considerable potential.

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Optimization of a growth medium using a statistical approach for the production of an alkaline protease from a newly isolated Bacillus sp. L21

Cited by 91 publications

References 21 publications

Optimization of biomass, pellet size and polygalacturonase production by Aspergillus sojae ATCC 20235 using response surface methodology

Optimization of biomass, pellet size and polygalacturonase production by Aspergillus sojae ATCC 20235 using response surface methodology

Alkaline protease production from alkalophilic Bacillus sp. isolated from natural habitats

A Bacillus Strain Able to Hydrolyze Alpha- and Beta-Keratin

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