2022
DOI: 10.1021/acs.analchem.2c03602
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Optimization of a Single Substrate-Based Fluorometric Assay for Glucose and Lactate Measurement to Assess Preimplantation Single Embryo Quality and Blood in Obese Mouse and Clinical Human Samples

Abstract: A fluorometric assay for single substrate-based glucose and lactate measurements was demonstrated by adopting an already established protocol for glucose and by optimizing pH, enzyme concentration, substrate concentration, and types of buffers for lactate. Linear calibration curves for glucose and lactate concentrations from 1 to 100 μM were obtained with correlation coefficients (R 2) of 0.94 and 0.98, respectively. First, with the optimized protocol, single embryo quality was successfully evaluated. Three di… Show more

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